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学科主题: 基础医学
题名:
Does not hUTP14a promoter form a regulation feedback loop with P53?
作者: Zhang, Jingyi1; Guo, Yafei2; Du, Xiaojuan2; Xing, Baocai1
关键词: hUTP14a ; luciferase activity ; promoter ; P53 ; transcription factor
刊名: CHINESE JOURNAL OF CANCER RESEARCH
发表日期: 2014-04-01
DOI: 10.3978/j.issn.1000-9604.2014.03.03
卷: 26, 期:2, 页:159-165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: UBIQUITIN LIGASE ; DEGRADATION ; CANCER ; PIRH2 ; GENE ; MDM2 ; COP1
英文摘要:

Objective: We previously found that hUTP14a binds P53 and promotes P53 degradation. However, if hUTP14a is a downstream gene of P53 remains to be determined. This study aimed to identify the promoter of hUTP14a and investigate if hUTP14a is regulated by P53.

Methods: The hUTP14a promoter region was cloned into pGL3-Basic-luciferase reporter plasmid to get pGL3-hUTP14a-luc. The reporter plasmid was transfected into 293T cells and luciferase activity was evaluated by the Dual-Luciferase Reporter Assay System. Putative transcription factors were identified through searching MatInspector Professional and Algorismica i Genetica databases. Either pGL3-hUTP14a-luc or p21 promoter reporter plasmid was co-transfected with increasing dose of p53 plasmid, and luciferase activity was evaluated. A series of deletion constructs of pGL3-hUTP14a-luc were constructed and minimal promoter region of hUTP14a was determined. Differences of the luciferase activities between different groups were assessed by statistical analysis.

Results: The hUTP14a gene promoter reporter construct was correctly cloned and was demonstrated to possess promoter activity. The transcription of hUTP14a was not regulated by P53. The minimal promoter region of hUTP14a gene is located between -203 to -100 of the transcription initiation site.

Conclusion: Unlike other P53-interacting proteins such as MDM2, Pirh2 and Cop I which promote P53 degradation and whose transcriptions are regulated by P53, does not hUTP14a transcription form a regulation feedback loop with P53.

语种: 英语
所属项目编号: 7122032 ; 81071672 ; 2010CB529303 ; 2008AA02Z131
项目资助者: Beijing Municipal Natural Science Foundation ; National Natural Science Foundation of China ; National Basic Research Program of China (973 program) ; National High Technology Research and Development Program of China (863 Program)
WOS记录号: WOS:000335622200006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55507
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Peking Univ Canc Hosp & Inst, Dept Hepat Biliary & Pancreat Surg 1, Key Lab Carcinogenesis & Translat Res, Minist Educ, Beijing 100142, Peoples R China
2.Peking Univ Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Jingyi,Guo, Yafei,Du, Xiaojuan,et al. Does not hUTP14a promoter form a regulation feedback loop with P53?[J]. CHINESE JOURNAL OF CANCER RESEARCH,2014,26(2):159-165.
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