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学科主题: 临床医学
题名:
Minocycline inhibits LPS-induced retinal microglia activation
作者: Wang, AL; Yu, ACH; Lau, LT; Lee, C; Wu, LM; Zhu, XA; Tso, MOM
关键词: microglia ; retina ; neurodegenerative disease ; LPS ; IL-lbeta ; TNF-alpha ; NO ; minocycline
刊名: NEUROCHEMISTRY INTERNATIONAL
发表日期: 2005-07-01
DOI: 10.1016/j.neuint.2005.04.018
卷: 47, 期:1-2, 页:152-158
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]: NITRIC-OXIDE SYNTHASE ; CYTOCHROME-C RELEASE ; MOUSE MODEL ; HUNTINGTONS-DISEASE ; SPINAL-CORD ; PHOTORECEPTORS ; RATS ; EXPRESSION ; CULTURE ; INJURY
英文摘要:

Retinal neurode-enerative disease involves an inflammatory response in the retina characterized by an increase in inflammatory cytokines and activation of microglia. The degree of microglia activation may influence the extent of retinal injury following an inflammatory stimulus. Cytokines released by activated microglia regulate the influx of inflammatory cells to the damaged area. Thus, a therapeutic strategy to reduce cytokine expression in microglia would be neuroprotective. Minocycline, a semisynthetic tetracycline derivative, is known to protect rodent brain from ischemia and to inhibit microglial activation. In this study, we activated retinal microglia in culture with lipopolysaccharide (LPS) and attempted to determine whether minocycline could reduce the production of cytokines from activated microglia at both gene and protein levels. Changes in inflammatory cytokines, TNF-alpha and IL-1beta, were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) in the presence or absence of LPS. We also measured the levels of nitric oxide (NO) by the nitrate reductase method under similar conditions. LPS treatment induced a significant upregulation of the mRNA and release of TNF-alpha, IL-1beta, and NO from retinal microglia. Minocycline inhibited these releases. Thus, minocycline might exert its antiinflammatory effect on microglia by inhibiting the expression and release of TNF-alpha, IL-lbeta, and NO. (c) 2005 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000230567400020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55515
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Eye Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Neurosci, Beijing 100083, Peoples R China
4.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
7.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21287 USA

Recommended Citation:
Wang, AL,Yu, ACH,Lau, LT,et al. Minocycline inhibits LPS-induced retinal microglia activation[J]. NEUROCHEMISTRY INTERNATIONAL,2005,47(1-2):152-158.
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