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学科主题临床医学
Minocycline inhibits LPS-induced retinal microglia activation
Wang, AL; Yu, ACH; Lau, LT; Lee, C; Wu, LM; Zhu, XA; Tso, MOM
关键词Microglia Retina Neurodegenerative Disease Lps Il-lbeta Tnf-alpha No Minocycline
刊名NEUROCHEMISTRY INTERNATIONAL
2005-07-01
DOI10.1016/j.neuint.2005.04.018
47期:1-2页:152-158
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]NITRIC-OXIDE SYNTHASE ; CYTOCHROME-C RELEASE ; MOUSE MODEL ; HUNTINGTONS-DISEASE ; SPINAL-CORD ; PHOTORECEPTORS ; RATS ; EXPRESSION ; CULTURE ; INJURY
英文摘要

Retinal neurode-enerative disease involves an inflammatory response in the retina characterized by an increase in inflammatory cytokines and activation of microglia. The degree of microglia activation may influence the extent of retinal injury following an inflammatory stimulus. Cytokines released by activated microglia regulate the influx of inflammatory cells to the damaged area. Thus, a therapeutic strategy to reduce cytokine expression in microglia would be neuroprotective. Minocycline, a semisynthetic tetracycline derivative, is known to protect rodent brain from ischemia and to inhibit microglial activation. In this study, we activated retinal microglia in culture with lipopolysaccharide (LPS) and attempted to determine whether minocycline could reduce the production of cytokines from activated microglia at both gene and protein levels. Changes in inflammatory cytokines, TNF-alpha and IL-1beta, were measured by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) in the presence or absence of LPS. We also measured the levels of nitric oxide (NO) by the nitrate reductase method under similar conditions. LPS treatment induced a significant upregulation of the mRNA and release of TNF-alpha, IL-1beta, and NO from retinal microglia. Minocycline inhibited these releases. Thus, minocycline might exert its antiinflammatory effect on microglia by inhibiting the expression and release of TNF-alpha, IL-lbeta, and NO. (c) 2005 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000230567400020
引用统计
被引频次:109[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55515
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Eye Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Neurosci Res Inst, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Key Lab Neurosci, Beijing 100083, Peoples R China
4.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100083, Peoples R China
7.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21287 USA
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GB/T 7714
Wang, AL,Yu, ACH,Lau, LT,et al. Minocycline inhibits LPS-induced retinal microglia activation[J]. NEUROCHEMISTRY INTERNATIONAL,2005,47(1-2):152-158.
APA Wang, AL.,Yu, ACH.,Lau, LT.,Lee, C.,Wu, LM.,...&Tso, MOM.(2005).Minocycline inhibits LPS-induced retinal microglia activation.NEUROCHEMISTRY INTERNATIONAL,47(1-2),152-158.
MLA Wang, AL,et al."Minocycline inhibits LPS-induced retinal microglia activation".NEUROCHEMISTRY INTERNATIONAL 47.1-2(2005):152-158.
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