|Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma|
|Dong, Wei-Wei1; Mou, Quan2; Chen, Jian2; Cui, Jian-Tao3; Li, Wen-Mei3; Xiao, Wen-Hua1|
|关键词||Rab27a Rab27b Hepatocellular Carcinoma Immunohisto-chemistry Prognosis|
|刊名||WORLD JOURNAL OF GASTROENTEROLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||BREAST-CANCER CELLS ; RAB GTPASES ; REGULATED EXOCYTOSIS ; GRISCELLI-SYNDROME ; GROWTH ; GENE ; MEMBRANE ; EXOSOMES ; METASTASIS ; SECRETION|
AIM: To investigate the association of Rab27A and Rab27B expression with clinicopathological characteristics and prognosis of hepatocellular carcinoma (HCC).
METHODS: We used reverse transcription polymerase chain reaction (RT-PCR), real-time PCR, and Western blotting to detect Rab27A and Rab27B mRNA and protein expression in 5 human HCC lines and the immortalized hepatic HL-7702 cell line. We further examined 148 primary HCC samples matched with adjacent normal tissue and 80 non-HCC specimens by immunohistochemistry to evaluate the correlation of Rab27A and Rab27B expression with clinicopathological features and prognosis.
RESULTS: Our data showed that Rab27A and Rab27B were differentially expressed in cell lines and primary HCC tumors. Rab27A mRNA and protein were detected in 67% (4/6) of human cell lines and 80% (4/5) of HCC cell lines, while Rab27B was found in 50% (3/6) of human lines and 40% (2/5) of HCC lines. Rab27A expression was higher in primary HCC (46.2%, 66/143) than in matched adjacent tissue (24.3%, 33/136, P < 0.001), whereas immunopositivity for Rab27B was lower in primary HCC (57.4%, 81/141) than in matched adjacent tissue (87.5%, 119/136, P < 0.001). Analysis of clinicopathological characteristics of 148 HCC specimens revealed significant correlations between Rab27A and Rab27B expression and tumor tumor-node-metastasis (TNM) classification (P = 0.046 and P = 0.027, respectively), and between strong Rab27A expression and tumor differentiation grade (P = 0.008). Survival analyses revealed that patients with Rab27A(+) or Rab27B(+) tumors had significantly reduced overall survival compared with that of patients with Rab27A(-) or Rab27B(-) tumors (P = 0.015 and P = 0.005, respectively). Risk analyses revealed that Rab27B(+) and TNM III-IV were independent poor prognosis factors associated with a 3.36- and 3.37-fold higher relative risk of death, respectively.
CONCLUSION: Rab27A and Rab27B expression were closely correlated with tumor progression and can be valuable prognostic indicators for HCC patients. (C) 2012 Baishideng. All rights reserved.
|作者单位||1.Chinese Peoples Liberat Army, Gen Hosp, Affiliated Hosp 1, Dept Oncol, Beijing 100048, Peoples R China|
2.Chinese Peoples Liberat Army, Hosp 252, Dept Surg, Baoding 071000, Hebei Province, Peoples R China
3.Peking Univ, Canc Hosp Inst, Mol Oncol Lab, Beijing 100142, Peoples R China
|Dong, Wei-Wei,Mou, Quan,Chen, Jian,et al. Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2012,18(15):1806-1813.|
|APA||Dong, Wei-Wei,Mou, Quan,Chen, Jian,Cui, Jian-Tao,Li, Wen-Mei,&Xiao, Wen-Hua.(2012).Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma.WORLD JOURNAL OF GASTROENTEROLOGY,18(15),1806-1813.|
|MLA||Dong, Wei-Wei,et al."Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma".WORLD JOURNAL OF GASTROENTEROLOGY 18.15(2012):1806-1813.|