北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室  > 期刊论文
学科主题: 临床医学
题名:
Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma
作者: Dong, Wei-Wei1; Mou, Quan2; Chen, Jian2; Cui, Jian-Tao3; Li, Wen-Mei3; Xiao, Wen-Hua1
关键词: Rab27A ; Rab27B ; Hepatocellular carcinoma ; Immunohisto-chemistry ; Prognosis
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2012-04-21
DOI: 10.3748/wjg.v18.i15.1806
卷: 18, 期:15, 页:1806-1813
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: BREAST-CANCER CELLS ; RAB GTPASES ; REGULATED EXOCYTOSIS ; GRISCELLI-SYNDROME ; GROWTH ; GENE ; MEMBRANE ; EXOSOMES ; METASTASIS ; SECRETION
英文摘要:

AIM: To investigate the association of Rab27A and Rab27B expression with clinicopathological characteristics and prognosis of hepatocellular carcinoma (HCC).

METHODS: We used reverse transcription polymerase chain reaction (RT-PCR), real-time PCR, and Western blotting to detect Rab27A and Rab27B mRNA and protein expression in 5 human HCC lines and the immortalized hepatic HL-7702 cell line. We further examined 148 primary HCC samples matched with adjacent normal tissue and 80 non-HCC specimens by immunohistochemistry to evaluate the correlation of Rab27A and Rab27B expression with clinicopathological features and prognosis.

RESULTS: Our data showed that Rab27A and Rab27B were differentially expressed in cell lines and primary HCC tumors. Rab27A mRNA and protein were detected in 67% (4/6) of human cell lines and 80% (4/5) of HCC cell lines, while Rab27B was found in 50% (3/6) of human lines and 40% (2/5) of HCC lines. Rab27A expression was higher in primary HCC (46.2%, 66/143) than in matched adjacent tissue (24.3%, 33/136, P < 0.001), whereas immunopositivity for Rab27B was lower in primary HCC (57.4%, 81/141) than in matched adjacent tissue (87.5%, 119/136, P < 0.001). Analysis of clinicopathological characteristics of 148 HCC specimens revealed significant correlations between Rab27A and Rab27B expression and tumor tumor-node-metastasis (TNM) classification (P = 0.046 and P = 0.027, respectively), and between strong Rab27A expression and tumor differentiation grade (P = 0.008). Survival analyses revealed that patients with Rab27A(+) or Rab27B(+) tumors had significantly reduced overall survival compared with that of patients with Rab27A(-) or Rab27B(-) tumors (P = 0.015 and P = 0.005, respectively). Risk analyses revealed that Rab27B(+) and TNM III-IV were independent poor prognosis factors associated with a 3.36- and 3.37-fold higher relative risk of death, respectively.

CONCLUSION: Rab27A and Rab27B expression were closely correlated with tumor progression and can be valuable prognostic indicators for HCC patients. (C) 2012 Baishideng. All rights reserved.

语种: 英语
WOS记录号: WOS:000303278400013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55524
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Peoples Liberat Army, Gen Hosp, Affiliated Hosp 1, Dept Oncol, Beijing 100048, Peoples R China
2.Chinese Peoples Liberat Army, Hosp 252, Dept Surg, Baoding 071000, Hebei Province, Peoples R China
3.Peking Univ, Canc Hosp Inst, Mol Oncol Lab, Beijing 100142, Peoples R China

Recommended Citation:
Dong, Wei-Wei,Mou, Quan,Chen, Jian,et al. Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2012,18(15):1806-1813.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Dong, Wei-Wei]'s Articles
[Mou, Quan]'s Articles
[Chen, Jian]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Dong, Wei-Wei]‘s Articles
[Mou, Quan]‘s Articles
[Chen, Jian]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace