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学科主题: 临床医学
题名:
Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma
作者: Dong, Wei-Wei1; Mou, Quan2; Chen, Jian2; Cui, Jian-Tao3; Li, Wen-Mei3; Xiao, Wen-Hua1
关键词: Rab27A ; Rab27B ; Hepatocellular carcinoma ; Immunohisto-chemistry ; Prognosis
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2012-04-21
DOI: 10.3748/wjg.v18.i15.1806
卷: 18, 期:15, 页:1806-1813
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: BREAST-CANCER CELLS ; RAB GTPASES ; REGULATED EXOCYTOSIS ; GRISCELLI-SYNDROME ; GROWTH ; GENE ; MEMBRANE ; EXOSOMES ; METASTASIS ; SECRETION
英文摘要:

AIM: To investigate the association of Rab27A and Rab27B expression with clinicopathological characteristics and prognosis of hepatocellular carcinoma (HCC).

METHODS: We used reverse transcription polymerase chain reaction (RT-PCR), real-time PCR, and Western blotting to detect Rab27A and Rab27B mRNA and protein expression in 5 human HCC lines and the immortalized hepatic HL-7702 cell line. We further examined 148 primary HCC samples matched with adjacent normal tissue and 80 non-HCC specimens by immunohistochemistry to evaluate the correlation of Rab27A and Rab27B expression with clinicopathological features and prognosis.

RESULTS: Our data showed that Rab27A and Rab27B were differentially expressed in cell lines and primary HCC tumors. Rab27A mRNA and protein were detected in 67% (4/6) of human cell lines and 80% (4/5) of HCC cell lines, while Rab27B was found in 50% (3/6) of human lines and 40% (2/5) of HCC lines. Rab27A expression was higher in primary HCC (46.2%, 66/143) than in matched adjacent tissue (24.3%, 33/136, P < 0.001), whereas immunopositivity for Rab27B was lower in primary HCC (57.4%, 81/141) than in matched adjacent tissue (87.5%, 119/136, P < 0.001). Analysis of clinicopathological characteristics of 148 HCC specimens revealed significant correlations between Rab27A and Rab27B expression and tumor tumor-node-metastasis (TNM) classification (P = 0.046 and P = 0.027, respectively), and between strong Rab27A expression and tumor differentiation grade (P = 0.008). Survival analyses revealed that patients with Rab27A(+) or Rab27B(+) tumors had significantly reduced overall survival compared with that of patients with Rab27A(-) or Rab27B(-) tumors (P = 0.015 and P = 0.005, respectively). Risk analyses revealed that Rab27B(+) and TNM III-IV were independent poor prognosis factors associated with a 3.36- and 3.37-fold higher relative risk of death, respectively.

CONCLUSION: Rab27A and Rab27B expression were closely correlated with tumor progression and can be valuable prognostic indicators for HCC patients. (C) 2012 Baishideng. All rights reserved.

语种: 英语
WOS记录号: WOS:000303278400013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55524
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

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作者单位: 1.Chinese Peoples Liberat Army, Gen Hosp, Affiliated Hosp 1, Dept Oncol, Beijing 100048, Peoples R China
2.Chinese Peoples Liberat Army, Hosp 252, Dept Surg, Baoding 071000, Hebei Province, Peoples R China
3.Peking Univ, Canc Hosp Inst, Mol Oncol Lab, Beijing 100142, Peoples R China

Recommended Citation:
Dong, Wei-Wei,Mou, Quan,Chen, Jian,et al. Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2012,18(15):1806-1813.
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