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学科主题: 临床医学
题名:
Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections
作者: Shinozaki, Minoru1; Okubo, Yoichiro1; Sasai, Daisuke1; Nakayama, Haruo1; Murayama, Somay Yamagata2; Ide, Tadashi1; Wakayama, Megumi1; Ishiwatari, Takao1; Tochigi, Naobumi1; Nemoto, Tetsuo1; Shibuya, Kazutoshi1,3
刊名: JOURNAL OF CLINICAL MICROBIOLOGY
发表日期: 2013
DOI: 10.1128/JCM.02221-12
卷: 51, 期:1, 页:295-298
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: MEDICALLY IMPORTANT FUNGI ; CANDIDA-ALBICANS ; NESTED PCR ; DNA ; DIFFERENTIATION ; ASPERGILLUS ; BEIGELII ; INFECTIONS ; POLYAMIDE ; DIAGNOSIS
英文摘要:

In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.

语种: 英语
所属项目编号: H22-Shinko-Ippan-8 ; H23-Shinko-Ippan-18 ; 24790364
项目资助者: Ministry of Health, Labor and Welfare of Japan ; Strategic Basis on Research Grounds for Non-Governmental Schools at Heisei ; KAKENHI from the Ministry of Education, Culture, Sports, Science and Technology of Japan ; Yokohama Foundation for Advancement of Medical Science ; Pfizer Inc.
WOS记录号: WOS:000314105100050
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55526
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Toho Univ Sch Med, Dept Surg Pathol, Tokyo, Japan
2.Nihon Univ, Sch Pharm, Mol Cell Biol Lab, Chiba, Japan
3.Peking Univ First Hosp, Dept Dermatol, Beijing, Peoples R China

Recommended Citation:
Shinozaki, Minoru,Okubo, Yoichiro,Sasai, Daisuke,et al. Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2013,51(1):295-298.
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