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学科主题临床医学
Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections
Shinozaki, Minoru1; Okubo, Yoichiro1; Sasai, Daisuke1; Nakayama, Haruo1; Murayama, Somay Yamagata2; Ide, Tadashi1; Wakayama, Megumi1; Ishiwatari, Takao1; Tochigi, Naobumi1; Nemoto, Tetsuo1; Shibuya, Kazutoshi1,3
刊名JOURNAL OF CLINICAL MICROBIOLOGY
2013
DOI10.1128/JCM.02221-12
51期:1页:295-298
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]MEDICALLY IMPORTANT FUNGI ; CANDIDA-ALBICANS ; NESTED PCR ; DNA ; DIFFERENTIATION ; ASPERGILLUS ; BEIGELII ; INFECTIONS ; POLYAMIDE ; DIAGNOSIS
英文摘要

In order to identify Trichosporon species in formalin-fixed and paraffin-embedded sections from which visual discrimination of non-glabrata Candida species is mostly ineffective but critical for the choice of antifungals, we tested the usefulness of a newly designed peptide nucleic acid probe (PNA) for in situ hybridization (ISH). Results confirmed the usefulness of ISH with our PNA probe in identifying Trichosporon species from Candida albicans.

语种英语
WOS记录号WOS:000314105100050
项目编号H22-Shinko-Ippan-8 ; H23-Shinko-Ippan-18 ; 24790364
资助机构Ministry of Health, Labor and Welfare of Japan ; Strategic Basis on Research Grounds for Non-Governmental Schools at Heisei ; KAKENHI from the Ministry of Education, Culture, Sports, Science and Technology of Japan ; Yokohama Foundation for Advancement of Medical Science ; Pfizer Inc.
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55526
专题北京大学第一临床医学院_皮肤性病科
作者单位1.Toho Univ Sch Med, Dept Surg Pathol, Tokyo, Japan
2.Nihon Univ, Sch Pharm, Mol Cell Biol Lab, Chiba, Japan
3.Peking Univ First Hosp, Dept Dermatol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Shinozaki, Minoru,Okubo, Yoichiro,Sasai, Daisuke,et al. Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections[J]. JOURNAL OF CLINICAL MICROBIOLOGY,2013,51(1):295-298.
APA Shinozaki, Minoru.,Okubo, Yoichiro.,Sasai, Daisuke.,Nakayama, Haruo.,Murayama, Somay Yamagata.,...&Shibuya, Kazutoshi.(2013).Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections.JOURNAL OF CLINICAL MICROBIOLOGY,51(1),295-298.
MLA Shinozaki, Minoru,et al."Development of a Peptide Nucleic Acid Probe to Trichosporon Species and Identification of Trichosporonosis by Use of In Situ Hybridization in Formalin-Fixed and Paraffin-Embedded (FFPE) Sections".JOURNAL OF CLINICAL MICROBIOLOGY 51.1(2013):295-298.
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