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学科主题: 临床医学
题名:
Adenovirus-mediated PDCD5 gene transfer sensitizes K562 cells to apoptosis induced by idarubicin in vitro and in vivo
作者: Ruan, Guo-Rui; Zhao, Hong-Shan3; Chang, Yan; Li, Jin-Lan; Qin, Ya-Zhen; Liu, Yan-Rong; Chen, Shan-Shan1; Huang, Xiao-Jun1
关键词: PDCD5 ; gene therapy ; adenoviral vector ; idarubicin ; leukemia ; nude mice
刊名: APOPTOSIS
发表日期: 2008-05-01
DOI: 10.1007/s10495-008-0206-9
卷: 13, 期:5, 页:641-648
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: TOPOISOMERASE-II ; EXPRESSION PROFILES ; BREAST-CANCER ; TUMOR-CELLS ; THERAPY ; LEUKEMIA ; MODEL ; INHIBITORS ; GROWTH ; TFAR19
英文摘要:

PDCD5 (programmed cell death 5) accelerates apoptosis of certain tumor cells and is expressed at low levels in marrow-nucleated cells of AML and CML patients. In the present study, we evaluated the effects of PDCD5 overexpression on drug sensitivity of leukemia cells. K562 cells were treated with idarubicin (IDR) alone or in combination with adenoviral vectors expressing PDCD5 (Ad-PDCD5). As shown by annexin-V-FITC/PI dual labeling, apoptosis rates were markedly increased after combined treatment with Ad-PDCD5 compared to IDR treatment alone. We observed that PDCD5 overexpression significantly improves the antitumor effects of low dose IDR treatment in vivo. Tumor sizes were significantly decreased in combined Ad-PDCD5 and low dose IDR treatment groups compared with single IDR treatment groups. Similar results were obtained with combined systemic treatment of Ad-PDCD5 and low dose IDR, and combined treatment with Ad-PDCD5 local injection and low dose IDR i.p. injection. These results indicate that Ad-PDCD5 may be a promising agent for enhancing chemosensitivity.

语种: 英语
WOS记录号: WOS:000255030400004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55537
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Beijing 100044, Peoples R China
2.Inst Hematol, Beijing 100044, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China

Recommended Citation:
Ruan, Guo-Rui,Zhao, Hong-Shan,Chang, Yan,et al. Adenovirus-mediated PDCD5 gene transfer sensitizes K562 cells to apoptosis induced by idarubicin in vitro and in vivo[J]. APOPTOSIS,2008,13(5):641-648.
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