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学科主题: 药学
题名:
Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3
作者: Li, Lihong1; Zhang, Jinqian2; Yang, Yang1; Wang, Qiang1; Gao, Li1; Yang, Yanlong1; Chang, Tao1; Zhang, Xingye1; Xiang, Guoan3; Cao, Yongmei4; Shi, Zujin5; Zhao, Ming6; Gao, Guodong1
关键词: Single-walled carbon nanohorns ; Septic encephalopathy ; Cell proliferation ; Apoptosis ; Lipopolysaccharide ; Sepsis ; Sirt3
刊名: NANOSCALE RESEARCH LETTERS
发表日期: 2013-02-22
DOI: 10.1186/1556-276X-8-100
卷: 8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]: Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]: MS/MS PROTEOME ANALYSIS ; HEPATOMA HEPG2 CELLS ; IN-VITRO TOXICITY ; NAD(+) DEPLETION ; OXIDATIVE STRESS ; MACROPHAGE CELLS ; DRUG CARRIERS ; NANOTUBES ; CYTOTOXICITY ; DEATH
英文摘要:

Single-wall carbon nanohorns (SWNHs) have been demonstrated to accumulate in cytotoxic levels within organs of various animal models and cell types, which emerge as a wide range of promising biomedical imaging. Septic encephalopathy (SE) is an early sign of sepsis and associated with an increased rate of morbidity and mortality. Microglia activation plays an important role in neuroinflammation, which contributes to neuronal damage. Inhibition of microglia activation may have therapeutic benefits, which can alleviate the progression of neurodegeneration. Therefore, we investigated the functional changes of mice microglia cell lines pre-treated with or without lipopolysaccharide (LPS) induced by SWNHs. To address this question, the research about direct role of SWNHs on the growth, proliferation, and apoptosis of microglia cell lines in mice (N9 and BV2) pre-treated with or without LPS had been performed. Our results indicate that the particle diameter of SWNHs in water is between 342 to 712 nm. The images in scanning electron microscope showed that SWNHs on polystyrene surface are individual particles. LPS induced activation of mice microglia, promoted its growth and proliferation, and inhibited its apoptosis. SWNHs inhibited proliferation, delayed mitotic entry, and promoted apoptosis of mice microglia cells. The effects followed gradually increasing cultured time and concentrations of SWNHs, especially in cells pre-treated with LPS. SWNHs induced a significantly increase in G1 phase and inhibition of S phase of mice microglia cells in a dose-manner dependent of SWNHs, especially in cells pre-treated with LPS. The transmission electron microscope images showed that individual spherical SWNH particles smaller than 100 nm in diameters were localized inside lysosomes of mice microglia cells. SWNHs inhibited mitotic entry, growth and proliferation of mice microglia cells, and promoted its apoptosis, especially in cells pre-treated with LPS. SWNHs inhibited expression of Sirt3 and energy metabolism related with Sirt3 in mice microglia cells in a dose-dependent manner, especially in cells pre-treated with LPS. The role of SWNHs on mice microglia was implicating Sirt3 and energy metabolism associated with it.

语种: 英语
所属项目编号: 30600524 ; 81071990 ; 81172383 ; 81201758 ; 2012A030400055 ; 2010B080701088 ; 2011B080701096 ; 2011B031800184 ; 2011J410010 ; 2011J4300066
项目资助者: National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Science and Technology Application infrastructure projects of Guangzhou
WOS记录号: WOS:000315940800001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55543
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Int Mongolian Med Hosp Inner Mongolia, Hohhot 010065, Peoples R China
2.Fourth Mil Med Univ, Tangdu Hosp, Dept Neurosurg, Xian 710038, Peoples R China
3.Capital Med Univ, Beijing Ditan Hosp, Inst Infect Dis, Beijing 100015, Peoples R China
4.Second Peoples Hosp Guangdong Prov, Dept Gen Surg, Guangzhou 510515, Guangdong, Peoples R China
5.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Rare Earth Mat Chem & Applicat, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
6.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China

Recommended Citation:
Li, Lihong,Zhang, Jinqian,Yang, Yang,et al. Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3[J]. NANOSCALE RESEARCH LETTERS,2013,8.
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