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Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3
Li, Lihong1; Zhang, Jinqian2; Yang, Yang1; Wang, Qiang1; Gao, Li1; Yang, Yanlong1; Chang, Tao1; Zhang, Xingye1; Xiang, Guoan3; Cao, Yongmei4; Shi, Zujin5; Zhao, Ming6; Gao, Guodong1
关键词Single-walled Carbon Nanohorns Septic Encephalopathy Cell Proliferation Apoptosis Lipopolysaccharide Sepsis Sirt3
刊名NANOSCALE RESEARCH LETTERS
2013-02-22
DOI10.1186/1556-276X-8-100
8
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
研究领域[WOS]Science & Technology - Other Topics ; Materials Science ; Physics
关键词[WOS]MS/MS PROTEOME ANALYSIS ; HEPATOMA HEPG2 CELLS ; IN-VITRO TOXICITY ; NAD(+) DEPLETION ; OXIDATIVE STRESS ; MACROPHAGE CELLS ; DRUG CARRIERS ; NANOTUBES ; CYTOTOXICITY ; DEATH
英文摘要

Single-wall carbon nanohorns (SWNHs) have been demonstrated to accumulate in cytotoxic levels within organs of various animal models and cell types, which emerge as a wide range of promising biomedical imaging. Septic encephalopathy (SE) is an early sign of sepsis and associated with an increased rate of morbidity and mortality. Microglia activation plays an important role in neuroinflammation, which contributes to neuronal damage. Inhibition of microglia activation may have therapeutic benefits, which can alleviate the progression of neurodegeneration. Therefore, we investigated the functional changes of mice microglia cell lines pre-treated with or without lipopolysaccharide (LPS) induced by SWNHs. To address this question, the research about direct role of SWNHs on the growth, proliferation, and apoptosis of microglia cell lines in mice (N9 and BV2) pre-treated with or without LPS had been performed. Our results indicate that the particle diameter of SWNHs in water is between 342 to 712 nm. The images in scanning electron microscope showed that SWNHs on polystyrene surface are individual particles. LPS induced activation of mice microglia, promoted its growth and proliferation, and inhibited its apoptosis. SWNHs inhibited proliferation, delayed mitotic entry, and promoted apoptosis of mice microglia cells. The effects followed gradually increasing cultured time and concentrations of SWNHs, especially in cells pre-treated with LPS. SWNHs induced a significantly increase in G1 phase and inhibition of S phase of mice microglia cells in a dose-manner dependent of SWNHs, especially in cells pre-treated with LPS. The transmission electron microscope images showed that individual spherical SWNH particles smaller than 100 nm in diameters were localized inside lysosomes of mice microglia cells. SWNHs inhibited mitotic entry, growth and proliferation of mice microglia cells, and promoted its apoptosis, especially in cells pre-treated with LPS. SWNHs inhibited expression of Sirt3 and energy metabolism related with Sirt3 in mice microglia cells in a dose-dependent manner, especially in cells pre-treated with LPS. The role of SWNHs on mice microglia was implicating Sirt3 and energy metabolism associated with it.

语种英语
WOS记录号WOS:000315940800001
项目编号30600524 ; 81071990 ; 81172383 ; 81201758 ; 2012A030400055 ; 2010B080701088 ; 2011B080701096 ; 2011B031800184 ; 2011J410010 ; 2011J4300066
资助机构National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Science and Technology Application infrastructure projects of Guangzhou
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55543
专题北京大学药学院_化学生物学系
作者单位1.Int Mongolian Med Hosp Inner Mongolia, Hohhot 010065, Peoples R China
2.Fourth Mil Med Univ, Tangdu Hosp, Dept Neurosurg, Xian 710038, Peoples R China
3.Capital Med Univ, Beijing Ditan Hosp, Inst Infect Dis, Beijing 100015, Peoples R China
4.Second Peoples Hosp Guangdong Prov, Dept Gen Surg, Guangzhou 510515, Guangdong, Peoples R China
5.Peking Univ, Beijing Natl Lab Mol Sci, State Key Lab Rare Earth Mat Chem & Applicat, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
6.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100191, Peoples R China
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GB/T 7714
Li, Lihong,Zhang, Jinqian,Yang, Yang,et al. Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3[J]. NANOSCALE RESEARCH LETTERS,2013,8.
APA Li, Lihong.,Zhang, Jinqian.,Yang, Yang.,Wang, Qiang.,Gao, Li.,...&Gao, Guodong.(2013).Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3.NANOSCALE RESEARCH LETTERS,8.
MLA Li, Lihong,et al."Single-wall carbon nanohorns inhibited activation of microglia induced by lipopolysaccharide through blocking of Sirt3".NANOSCALE RESEARCH LETTERS 8(2013).
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