北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 期刊论文
学科主题: 基础医学
题名:
Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells
作者: Jiang, H; Wang, XF; Fang, L; Tang, CS; Zhu, Y; Wang, X
关键词: homocysteine ; aldose reductase ; surfactant protein B ; reactive oxygen species
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2005-12-02
DOI: 10.1016/j.bbrc.2005.09.160
卷: 337, 期:4, 页:1084-1091
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: SURFACTANT PROTEIN-B ; RISK-FACTOR ; VASCULAR-DISEASE ; PLASMA HOMOCYSTEINE ; OXIDATIVE STRESS ; HYPERHOMOCYSTEINEMIA ; INJURY ; METABOLISM ; EXPRESSION ; INDUCTION
英文摘要:

Homocysteine (Hcy) has recently been recognized as an integral component of several disorders. However, the association between hyperhomocysteinemia (HHcy) and pulmonary disease is not well understood. ]The combination of two-dimensional electrophoresis and tandem mass spectrometry detected and identified proteins that are differentially expressed in human type 11 alveolar epithelial cells (A549 cells) treated by Hey. We found that aldose reductase (AR) showed more abundant expression in the cells, Further, Hey (100500 mu M) could induce a time- and dose-dependent upregulation of AR protein levels. Immunohistochemical staining of cross-sections from HHcy mice lungs also revealed increased expression of AR protein. Intracellular levels of reactive oxygen species (ROS) were remarkably elevated in A549 cells treated with Hey. Pretreatment of A549 cells with catalase and SOD significantly suppressed the Hcy-induced AR expression, which suggests the involvement of ROS in this process. The major signaling pathway mediating the upregulation of AR was demonstrated to be the Ras/Raf/ERK1/2 pathway. In addition, Hey might reduce surfactant protein B (SP-B) expression in the cells, which could be significantly attenuated by Alrestatin, an AR inhibitor, indicating a damaging role of Hcy-induced AR elevation in the lung. These results show a novel and unanticipated link between HHcy and AR upregulation that may be a risk factor in pulmonary disease of patients with HHcy. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000233120600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55547
Appears in Collections:基础医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100083, Peoples R China

Recommended Citation:
Jiang, H,Wang, XF,Fang, L,et al. Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,337(4):1084-1091.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Jiang, H]'s Articles
[Wang, XF]'s Articles
[Fang, L]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Jiang, H]‘s Articles
[Wang, XF]‘s Articles
[Fang, L]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace