Institutional Repository of Peking University School of Basic Medical Sciences
学科主题 | 基础医学 |
Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells | |
Jiang, H; Wang, XF; Fang, L; Tang, CS; Zhu, Y; Wang, X | |
关键词 | Homocysteine Aldose Reductase Surfactant Protein b Reactive Oxygen Species |
刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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2005-12-02 | |
DOI | 10.1016/j.bbrc.2005.09.160 |
卷 | 337期:4页:1084-1091 |
收录类别 | SCI |
文章类型 | Article |
WOS标题词 | Science & Technology |
类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
关键词[WOS] | SURFACTANT PROTEIN-B ; RISK-FACTOR ; VASCULAR-DISEASE ; PLASMA HOMOCYSTEINE ; OXIDATIVE STRESS ; HYPERHOMOCYSTEINEMIA ; INJURY ; METABOLISM ; EXPRESSION ; INDUCTION |
英文摘要 | Homocysteine (Hcy) has recently been recognized as an integral component of several disorders. However, the association between hyperhomocysteinemia (HHcy) and pulmonary disease is not well understood. ]The combination of two-dimensional electrophoresis and tandem mass spectrometry detected and identified proteins that are differentially expressed in human type 11 alveolar epithelial cells (A549 cells) treated by Hey. We found that aldose reductase (AR) showed more abundant expression in the cells, Further, Hey (100500 mu M) could induce a time- and dose-dependent upregulation of AR protein levels. Immunohistochemical staining of cross-sections from HHcy mice lungs also revealed increased expression of AR protein. Intracellular levels of reactive oxygen species (ROS) were remarkably elevated in A549 cells treated with Hey. Pretreatment of A549 cells with catalase and SOD significantly suppressed the Hcy-induced AR expression, which suggests the involvement of ROS in this process. The major signaling pathway mediating the upregulation of AR was demonstrated to be the Ras/Raf/ERK1/2 pathway. In addition, Hey might reduce surfactant protein B (SP-B) expression in the cells, which could be significantly attenuated by Alrestatin, an AR inhibitor, indicating a damaging role of Hcy-induced AR elevation in the lung. These results show a novel and unanticipated link between HHcy and AR upregulation that may be a risk factor in pulmonary disease of patients with HHcy. (c) 2005 Elsevier Inc. All rights reserved. |
语种 | 英语 |
WOS记录号 | WOS:000233120600010 |
Citation statistics | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.bjmu.edu.cn/handle/400002259/55547 |
Collection | 北京大学基础医学院 |
作者单位 | Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100083, Peoples R China |
Recommended Citation GB/T 7714 | Jiang, H,Wang, XF,Fang, L,et al. Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,337(4):1084-1091. |
APA | Jiang, H,Wang, XF,Fang, L,Tang, CS,Zhu, Y,&Wang, X.(2005).Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,337(4),1084-1091. |
MLA | Jiang, H,et al."Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 337.4(2005):1084-1091. |
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