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学科主题基础医学
Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells
Jiang, H; Wang, XF; Fang, L; Tang, CS; Zhu, Y; Wang, X
关键词Homocysteine Aldose Reductase Surfactant Protein b Reactive Oxygen Species
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2005-12-02
DOI10.1016/j.bbrc.2005.09.160
337期:4页:1084-1091
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]SURFACTANT PROTEIN-B ; RISK-FACTOR ; VASCULAR-DISEASE ; PLASMA HOMOCYSTEINE ; OXIDATIVE STRESS ; HYPERHOMOCYSTEINEMIA ; INJURY ; METABOLISM ; EXPRESSION ; INDUCTION
英文摘要

Homocysteine (Hcy) has recently been recognized as an integral component of several disorders. However, the association between hyperhomocysteinemia (HHcy) and pulmonary disease is not well understood. ]The combination of two-dimensional electrophoresis and tandem mass spectrometry detected and identified proteins that are differentially expressed in human type 11 alveolar epithelial cells (A549 cells) treated by Hey. We found that aldose reductase (AR) showed more abundant expression in the cells, Further, Hey (100500 mu M) could induce a time- and dose-dependent upregulation of AR protein levels. Immunohistochemical staining of cross-sections from HHcy mice lungs also revealed increased expression of AR protein. Intracellular levels of reactive oxygen species (ROS) were remarkably elevated in A549 cells treated with Hey. Pretreatment of A549 cells with catalase and SOD significantly suppressed the Hcy-induced AR expression, which suggests the involvement of ROS in this process. The major signaling pathway mediating the upregulation of AR was demonstrated to be the Ras/Raf/ERK1/2 pathway. In addition, Hey might reduce surfactant protein B (SP-B) expression in the cells, which could be significantly attenuated by Alrestatin, an AR inhibitor, indicating a damaging role of Hcy-induced AR elevation in the lung. These results show a novel and unanticipated link between HHcy and AR upregulation that may be a risk factor in pulmonary disease of patients with HHcy. (c) 2005 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000233120600010
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55547
专题北京大学基础医学院
作者单位Peking Univ, Sch Basic Med Sci, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100083, Peoples R China
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GB/T 7714
Jiang, H,Wang, XF,Fang, L,et al. Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,337(4):1084-1091.
APA Jiang, H,Wang, XF,Fang, L,Tang, CS,Zhu, Y,&Wang, X.(2005).Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,337(4),1084-1091.
MLA Jiang, H,et al."Upregulation of aldose reductase by homocysteine in type II alveolar epithelial cells".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 337.4(2005):1084-1091.
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