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学科主题: 临床医学
题名:
LTB4 can directly stimulate human osteoclast formation from PBMC independent of RANKL
作者: Jiang, J; Lv, HS; Lin, JH; Jiang, DF; Chen, ZK
关键词: osteoclast ; leukotriene B4 ; PBMC ; TRAP staining ; bone resorption
刊名: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
发表日期: 2005
DOI: 10.1080/10731190500289784
卷: 33, 期:4, 页:391-403
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]: LEUKOTRIENE B-4 ; IN-VITRO ; OSTEOPROTEGERIN LIGAND ; DIFFERENTIATION FACTOR ; BONE-RESORPTION ; CELLS ; MECHANISM ; MACROPHAGES ; ACTIVATION ; RECEPTORS
英文摘要:

Leukotriene B4, as a kind of 5-lipoxygenase metabolite of arachidonic acid, is known to influence osteoclast formation and bone resorption. In order to determine whether Leukotriene B4 could directly stimulate human osteoclast differentiation and activation independent of RANKL (ODF), three different concentrations of Leukotriene B4 (10(-9)M, 10(-8)M, 10(-7)M) were added to the culture of CD14+ monocyte fraction of peripheral blood mononuclear cell (PBMC) in the presence of macrophage colony-stimulating factor (M-CSF). Under these conditions, Leukotriene B4 could induce multinucleated cells, which were positive for Tartrate-resistant acidic phosphatase (TRAP) staining and capable of bone resorption. Addition of osteoprotegerin (OPG) to PBMC cultures does not abrogate osteoclast formation induced by LTB4. Osteoclastogenesis induced by Leukotriene B4 were dose-dependently increased and weaker than that of RANKL. These results indicated that Leukotriene B4, elevated in many inflammatory diseases, is directly capable of inducing osteoclast formation by a RANKL-independent mechanism.

语种: 英语
WOS记录号: WOS:000233088100002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55560
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Arthritis Clin & Res Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Jiang, J,Lv, HS,Lin, JH,et al. LTB4 can directly stimulate human osteoclast formation from PBMC independent of RANKL[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2005,33(4):391-403.
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