IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
LTB4 can directly stimulate human osteoclast formation from PBMC independent of RANKL
Jiang, J; Lv, HS; Lin, JH; Jiang, DF; Chen, ZK
关键词Osteoclast Leukotriene B4 Pbmc Trap Staining Bone Resorption
刊名ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
2005
DOI10.1080/10731190500289784
33期:4页:391-403
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]LEUKOTRIENE B-4 ; IN-VITRO ; OSTEOPROTEGERIN LIGAND ; DIFFERENTIATION FACTOR ; BONE-RESORPTION ; CELLS ; MECHANISM ; MACROPHAGES ; ACTIVATION ; RECEPTORS
英文摘要

Leukotriene B4, as a kind of 5-lipoxygenase metabolite of arachidonic acid, is known to influence osteoclast formation and bone resorption. In order to determine whether Leukotriene B4 could directly stimulate human osteoclast differentiation and activation independent of RANKL (ODF), three different concentrations of Leukotriene B4 (10(-9)M, 10(-8)M, 10(-7)M) were added to the culture of CD14+ monocyte fraction of peripheral blood mononuclear cell (PBMC) in the presence of macrophage colony-stimulating factor (M-CSF). Under these conditions, Leukotriene B4 could induce multinucleated cells, which were positive for Tartrate-resistant acidic phosphatase (TRAP) staining and capable of bone resorption. Addition of osteoprotegerin (OPG) to PBMC cultures does not abrogate osteoclast formation induced by LTB4. Osteoclastogenesis induced by Leukotriene B4 were dose-dependently increased and weaker than that of RANKL. These results indicated that Leukotriene B4, elevated in many inflammatory diseases, is directly capable of inducing osteoclast formation by a RANKL-independent mechanism.

语种英语
WOS记录号WOS:000233088100002
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55560
Collection北京大学第二临床医学院
作者单位Peking Univ, Peoples Hosp, Arthritis Clin & Res Ctr, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Jiang, J,Lv, HS,Lin, JH,et al. LTB4 can directly stimulate human osteoclast formation from PBMC independent of RANKL[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2005,33(4):391-403.
APA Jiang, J,Lv, HS,Lin, JH,Jiang, DF,&Chen, ZK.(2005).LTB4 can directly stimulate human osteoclast formation from PBMC independent of RANKL.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,33(4),391-403.
MLA Jiang, J,et al."LTB4 can directly stimulate human osteoclast formation from PBMC independent of RANKL".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 33.4(2005):391-403.
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