IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis
Liu, Qiang1; Zhang, Xin1; Dai, Linghui1; Hu, Xiaoqing1; Zhu, Jingxian1; Li, La1; Zhou, Chunyan2; Ao, Yingfang1
刊名ARTHRITIS & RHEUMATOLOGY
2014-04-01
DOI10.1002/art.38309
66期:4页:969-978
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Rheumatology
研究领域[WOS]Rheumatology
关键词[WOS]EXPRESSION PROFILING REVEALS ; HUMAN ARTICULAR CHONDROCYTES ; GENE-EXPRESSION ; IN-VITRO ; MOUSE OOCYTES ; GLYCOSAMINOGLYCANS ; ENHANCER ; REGIONS ; BIOLOGY ; CELLS
英文摘要

Objective. Long noncoding RNAs (lncRNAs) play crucial regulatory roles in diverse biologic processes, but knowledge of lncRNAs in osteoarthritis (OA) is limited. The aim of this study was to identify lncRNA expression in articular cartilage and to explore the function of cartilage injury-related lncRNAs (lncRNA-CIR) in OA.

Methods. To identify lncRNAs specifically expressed in OA cartilage, we compared the expression of lncRNAs in OA cartilage with that in normal cartilage using microarray and quantitative polymerase chain reaction (qPCR) analyses. In OA cartilage, lncRNA-CIR was specifically, differentially, and highly expressed. The function of lncRNA-CIR was determined by silencing and overexpression in vitro. Extracellular matrix (ECM)-related molecules were detected by qPCR, Western blot, and immunofluorescence analyses.

Results. Up to 152 lncRNAs were found to be differentially expressed (> 8-fold) in OA and normal cartilage (82 lncRNAs more highly expressed and 70 less highly expressed in OA cartilage than in normal cartilage). A specific differentially expressed lncRNA-CIR was selected according to the results of the higher expression in OA cartilage and OA chondrocytes. The expression of lncRNA-CIR increased in chondrocytes with in vitro treatment with interleukin-1 beta and tumor necrosis factor alpha. Silencing of lncRNA-CIR by small interfering RNA promoted the formation of collagen and aggrecan and reduced the expression of matrix-degrading enzymes, such as MMP13 and ADAMTS5. The expression of collagen and aggrecan was reduced, whereas the expression of matrix-degrading enzymes was increased, after overexpression of lncRNA-CIR.

Conclusion. The results indicate that lncRNACIR contributes to ECM degradation and plays a key role in the pathogenesis of OA. We propose that lncRNA-CIR could be used as a potential target in OA therapy.

语种英语
WOS记录号WOS:000337361000022
引用统计
被引频次:49[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55575
专题北京大学第三临床医学院
北京大学基础医学院
北京大学第二临床医学院_骨关节科
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Liu, Qiang,Zhang, Xin,Dai, Linghui,et al. Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis[J]. ARTHRITIS & RHEUMATOLOGY,2014,66(4):969-978.
APA Liu, Qiang.,Zhang, Xin.,Dai, Linghui.,Hu, Xiaoqing.,Zhu, Jingxian.,...&Ao, Yingfang.(2014).Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis.ARTHRITIS & RHEUMATOLOGY,66(4),969-978.
MLA Liu, Qiang,et al."Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis".ARTHRITIS & RHEUMATOLOGY 66.4(2014):969-978.
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