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学科主题: 临床医学
题名:
Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis
作者: Liu, Qiang1; Zhang, Xin1; Dai, Linghui1; Hu, Xiaoqing1; Zhu, Jingxian1; Li, La1; Zhou, Chunyan2; Ao, Yingfang1
刊名: ARTHRITIS & RHEUMATOLOGY
发表日期: 2014-04-01
DOI: 10.1002/art.38309
卷: 66, 期:4, 页:969-978
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Rheumatology
研究领域[WOS]: Rheumatology
关键词[WOS]: EXPRESSION PROFILING REVEALS ; HUMAN ARTICULAR CHONDROCYTES ; GENE-EXPRESSION ; IN-VITRO ; MOUSE OOCYTES ; GLYCOSAMINOGLYCANS ; ENHANCER ; REGIONS ; BIOLOGY ; CELLS
英文摘要:

Objective. Long noncoding RNAs (lncRNAs) play crucial regulatory roles in diverse biologic processes, but knowledge of lncRNAs in osteoarthritis (OA) is limited. The aim of this study was to identify lncRNA expression in articular cartilage and to explore the function of cartilage injury-related lncRNAs (lncRNA-CIR) in OA.

Methods. To identify lncRNAs specifically expressed in OA cartilage, we compared the expression of lncRNAs in OA cartilage with that in normal cartilage using microarray and quantitative polymerase chain reaction (qPCR) analyses. In OA cartilage, lncRNA-CIR was specifically, differentially, and highly expressed. The function of lncRNA-CIR was determined by silencing and overexpression in vitro. Extracellular matrix (ECM)-related molecules were detected by qPCR, Western blot, and immunofluorescence analyses.

Results. Up to 152 lncRNAs were found to be differentially expressed (> 8-fold) in OA and normal cartilage (82 lncRNAs more highly expressed and 70 less highly expressed in OA cartilage than in normal cartilage). A specific differentially expressed lncRNA-CIR was selected according to the results of the higher expression in OA cartilage and OA chondrocytes. The expression of lncRNA-CIR increased in chondrocytes with in vitro treatment with interleukin-1 beta and tumor necrosis factor alpha. Silencing of lncRNA-CIR by small interfering RNA promoted the formation of collagen and aggrecan and reduced the expression of matrix-degrading enzymes, such as MMP13 and ADAMTS5. The expression of collagen and aggrecan was reduced, whereas the expression of matrix-degrading enzymes was increased, after overexpression of lncRNA-CIR.

Conclusion. The results indicate that lncRNACIR contributes to ECM degradation and plays a key role in the pathogenesis of OA. We propose that lncRNA-CIR could be used as a potential target in OA therapy.

语种: 英语
所属项目编号: 90919022 ; 81370236 ; 81371889 ; 20110001130001 ; B07001 ; bmu2009129-112
项目资助者: National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; 111 Project of China ; Ministry of Health of China through the Program of Sports Injury
WOS记录号: WOS:000337361000022
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55575
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Qiang,Zhang, Xin,Dai, Linghui,et al. Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis[J]. ARTHRITIS & RHEUMATOLOGY,2014,66(4):969-978.
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