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学科主题: 基础医学
题名:
Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: Characteristics and effects on rats
作者: Lu, XL; Zheng, CY; Shi, XD; Wang, YQ; Suo, XY; Yu, PZ; Xu, YH; Ma, TM; Su, ZG
关键词: bovine hemoglobin ; human serum albumin ; conjugate ; blood substitutes ; glutaraldehyde
刊名: ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
发表日期: 2005
DOI: 10.1081/BIO-200055841
卷: 33, 期:2, 页:83-99
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]: Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]: CROSS-LINKED HEMOGLOBIN ; SAFETY
英文摘要:

Conjugate of bovine hemoglobin (bHb) and human serum albumin (HSA) was prepared. The product was simply composed of 89.7% one-to-one Hb-HSA conjugate, 6.0% oligomer of Hb and HSA, 3.5% unconjugated HSA and 0.8% unconjugated Hb, with an average molecular weight of 157kD. The physicochemical characteristics were determined. Effects of single replacement on blood pressure and long-term survival of rats with 30% and 60% acute blood loss were studied, in comparison with Ringer-lactate solution, stroma-free hemoglobin (SFHb), 5% HSA in Ringer-lactate, whole blood and no resuscitation fluid. Results showed that Hb-HSA conjugate maintained the mean arterial pressure of rats to initial level with no pressor effect. Long-term effects of the replacement fluids on 30% bleeding rats showed that, for the group infused with Hb-HSA conjugate, histology of five major organs, heart, kidney, liver, spleen and lung, were essentially normal, similar to that of whole blood, while obviously renal side-effects appeared in other groups. The efficacy of the conjugate was further demonstrated by the resuscitation of lethal hemorrhagic shock rats (60% acute blood loss) with 100% survival rate (followed for 14 days), the same result as whole blood. The Hb-HSA conjugate can thus be another candidate for blood substitute in emergency.

语种: 英语
WOS记录号: WOS:000230198300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55576
Appears in Collections:基础医学院_生理学与病理生理学系_期刊论文

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作者单位: 1.Chinese Acad Sci, Natl Key Lab Biochem Engn, Inst Proc Engn, Beijing 100080, Peoples R China
2.Univ Sci & Technol, Dept Environm Engn, Beijing, Peoples R China
3.Beijing Univ Chem Technol, Dept Bioengn, Beijing, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Physiol, Beijing 100871, Peoples R China

Recommended Citation:
Lu, XL,Zheng, CY,Shi, XD,et al. Conjugate of bovine hemoglobin and human serum albumin as a candidate for blood substitute: Characteristics and effects on rats[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2005,33(2):83-99.
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