|Effects of exogenous urotensin II on vascular remodelling after balloon injury|
|Zhang, Li-Fang1,2; Ding, Wen-Hui1; Shi, Li-Bin1; Li, Kang1; Haom, Yan-Jie1; Ke, Yuan-Nan2; Tang, Zhao-Shu3|
|关键词||Arteries Balloon Angioplasty Extracellular Matrix Restenosis Urotensin Ii Vascular Remodelling|
|刊名||CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY|
|WOS标题词||Science & Technology|
|类目[WOS]||Pharmacology & Pharmacy ; Physiology|
|研究领域[WOS]||Pharmacology & Pharmacy ; Physiology|
|关键词[WOS]||RECEPTOR GPR14 ; RAT ; RESTENOSIS ; IMMUNOREACTIVITY ; PROLIFERATION ; HYPERTENSION ; ANGIOPLASTY ; EXPRESSION ; DISEASE ; CELLS|
P>1. Urotensin II (U-II) is a powerful vasoconstrictor peptide that stimulates cell proliferation. However, the systemic effects of U-II on cellular and extracellular matrix responses of vessel walls have not been investigated. The aim of the present study was to determine the effect of exogenous U-II on arterial neointimal hyperplasia after balloon injury.
2. A stenosis model of the thoracic aorta after balloon injury was established in male Wistar rats. Rats were divided into three groups (n = 5 in each): (i) uninjured; (ii) injured for 21 days; and (iii) injured and then treated with U-II (1 nmol/kg per h) via an osmotic minipump for 21 days. Another group of rats were killed on Days 7 and 14 after balloon injury for the analysis of in vitro collagen synthesis and secretion with U-II treated by [3H]-proline incorporation and determination of [3H]-hydroxyproline radioactivity, respectively.
3. Urotensin II immunoreactivity was 1.74-fold higher in vessels injured for 21 days than in uninjured vessels and mRNA levels of the urotensin UT receptor were upregulated by 55% following injury. After U-II treatment, the mRNA levels of the UT receptor were further upregulated (by 40%). In addition, U-II treatment increased the intimal area of injured aortas (13 +/- 5 vs 7 +/- 2% in group iii and ii, respectively), as well as increasing collagen content and cell proliferation. Protein levels of matrix metalloproteinase 1 were decreased in U-II-treated rats. In vitro, U-II treatment increased collagen synthesis and secretion in uninjured vessels in a concentration-dependent manner (10-10, 10-9 and 10-8 mol/L U-II), especially in injured aortas on Day 7 after injury.
4. In conclusion, exogenous U-II may upregulate mRNA levels of the UT receptor, as well as increase collagen and cell proliferation, all of which would contribute to intimal hyperplasia after angioplasty.
|项目编号||30270541 ; 30871066|
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Hosp 1, Div Cardiol, Dept Internal Med, Beijing 100034, Peoples R China|
2.China Japan Friendship Hosp, Dept Internal Med, Div Cardiol, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Dept Physiol & Pathophysiol,Key Lab Mol Cardiol, Beijing 100034, Peoples R China
|Zhang, Li-Fang,Ding, Wen-Hui,Shi, Li-Bin,et al. Effects of exogenous urotensin II on vascular remodelling after balloon injury[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2010,37(4):477-481.|
|APA||Zhang, Li-Fang.,Ding, Wen-Hui.,Shi, Li-Bin.,Li, Kang.,Haom, Yan-Jie.,...&Tang, Zhao-Shu.(2010).Effects of exogenous urotensin II on vascular remodelling after balloon injury.CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,37(4),477-481.|
|MLA||Zhang, Li-Fang,et al."Effects of exogenous urotensin II on vascular remodelling after balloon injury".CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 37.4(2010):477-481.|