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学科主题: 临床医学
题名:
Effects of exogenous urotensin II on vascular remodelling after balloon injury
作者: Zhang, Li-Fang1,2; Ding, Wen-Hui1; Shi, Li-Bin1; Li, Kang1; Haom, Yan-Jie1; Ke, Yuan-Nan2; Tang, Zhao-Shu3
关键词: arteries ; balloon angioplasty ; extracellular matrix ; restenosis ; urotensin II ; vascular remodelling
刊名: CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
发表日期: 2010-04-01
DOI: 10.1111/j.1440-1681.2009.05336.x
卷: 37, 期:4, 页:477-481
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Physiology
研究领域[WOS]: Pharmacology & Pharmacy ; Physiology
关键词[WOS]: RECEPTOR GPR14 ; RAT ; RESTENOSIS ; IMMUNOREACTIVITY ; PROLIFERATION ; HYPERTENSION ; ANGIOPLASTY ; EXPRESSION ; DISEASE ; CELLS
英文摘要:

P>1. Urotensin II (U-II) is a powerful vasoconstrictor peptide that stimulates cell proliferation. However, the systemic effects of U-II on cellular and extracellular matrix responses of vessel walls have not been investigated. The aim of the present study was to determine the effect of exogenous U-II on arterial neointimal hyperplasia after balloon injury.

2. A stenosis model of the thoracic aorta after balloon injury was established in male Wistar rats. Rats were divided into three groups (n = 5 in each): (i) uninjured; (ii) injured for 21 days; and (iii) injured and then treated with U-II (1 nmol/kg per h) via an osmotic minipump for 21 days. Another group of rats were killed on Days 7 and 14 after balloon injury for the analysis of in vitro collagen synthesis and secretion with U-II treated by [3H]-proline incorporation and determination of [3H]-hydroxyproline radioactivity, respectively.

3. Urotensin II immunoreactivity was 1.74-fold higher in vessels injured for 21 days than in uninjured vessels and mRNA levels of the urotensin UT receptor were upregulated by 55% following injury. After U-II treatment, the mRNA levels of the UT receptor were further upregulated (by 40%). In addition, U-II treatment increased the intimal area of injured aortas (13 +/- 5 vs 7 +/- 2% in group iii and ii, respectively), as well as increasing collagen content and cell proliferation. Protein levels of matrix metalloproteinase 1 were decreased in U-II-treated rats. In vitro, U-II treatment increased collagen synthesis and secretion in uninjured vessels in a concentration-dependent manner (10-10, 10-9 and 10-8 mol/L U-II), especially in injured aortas on Day 7 after injury.

4. In conclusion, exogenous U-II may upregulate mRNA levels of the UT receptor, as well as increase collagen and cell proliferation, all of which would contribute to intimal hyperplasia after angioplasty.

语种: 英语
所属项目编号: 30270541 ; 30871066
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000275766900014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55590
Appears in Collections:北京大学第一临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Div Cardiol, Dept Internal Med, Beijing 100034, Peoples R China
2.China Japan Friendship Hosp, Dept Internal Med, Div Cardiol, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Dept Physiol & Pathophysiol,Key Lab Mol Cardiol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Li-Fang,Ding, Wen-Hui,Shi, Li-Bin,et al. Effects of exogenous urotensin II on vascular remodelling after balloon injury[J]. CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY,2010,37(4):477-481.
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