IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
AGEs mediated expression and secretion of TNF alpha in rail retinal microglia
Wang, Ai Ling; Yu, Albert C. H.; He, Qi Hua; Zhu, Xiu′ An; Tso, Mark O. M.
关键词Ages Retinal Microglia Diabetic Retinopathy Tnf Alpha
刊名EXPERIMENTAL EYE RESEARCH
2007-05-01
DOI10.1016/j.exer.2007.01.011
84期:5页:905-913
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]GLYCATION END-PRODUCTS ; NITRIC-OXIDE SYNTHASE ; KAPPA-B ACTIVATION ; DIABETIC-RETINOPATHY ; GENE-EXPRESSION ; ALVEOLAR MACROPHAGES ; PARKINSONS-DISEASE ; ENDOTHELIAL-CELLS ; LIPOPOLYSACCHARIDE ; ENDPRODUCTS
英文摘要

Diabetic retinopathy induces an inflammatory response in the retina characterized by an increase in inflammatory cytokines and the activation of microglia. The degree of microglia activation may influence the extent of retina injury following retinal metabolic stress. We have previously shown that DR rats have elevated levels of advanced glycation end products (AGEs) in their blood. We have also suggested that AGEs might be involved in microglial activation and production of tumor necrosis factor alpha (TNF alpha). In this study, we attempted to confirm that AGEs induce the release of TNF alpha from rat retinal microglia using an in vitro microglia culture system, and concurrently to explore the mediating mechanisms. AGEs increased the protein secretion and mRNA expression of TNF alpha in cultured rat retinal microglia. These effects of AGEs were primarily mediated by reactive oxygen species (ROS). Furthermore, the inhibitors for mitogen-activated protein kinases (MAPK; p38, JNK and ERK 1/2) and nuclear factor-kB (NF-kB) could significantly decrease AGEs-induced TNF alpha release. AGEs-activated microglia showed an increase of NFkB p65 nuclear translocation. These observations indicated that pathophysiological levels of AGEs may alter rat retinal microglia function by upregulating TNF a expression and release via enhanced formation of intracellular ROS. AGEs-induced ROS subsequently activates MAPK (P38, JNK and ERK1/2) and NF-kB. (C) 2007 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000246534000013
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55593
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Eye Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Key Lab Neurosci, PKU, Neurosci Res Inst, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Beijing 100083, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China
6.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21218 USA
推荐引用方式
GB/T 7714
Wang, Ai Ling,Yu, Albert C. H.,He, Qi Hua,et al. AGEs mediated expression and secretion of TNF alpha in rail retinal microglia[J]. EXPERIMENTAL EYE RESEARCH,2007,84(5):905-913.
APA Wang, Ai Ling,Yu, Albert C. H.,He, Qi Hua,Zhu, Xiu&prime,An,&Tso, Mark O. M..(2007).AGEs mediated expression and secretion of TNF alpha in rail retinal microglia.EXPERIMENTAL EYE RESEARCH,84(5),905-913.
MLA Wang, Ai Ling,et al."AGEs mediated expression and secretion of TNF alpha in rail retinal microglia".EXPERIMENTAL EYE RESEARCH 84.5(2007):905-913.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Ai Ling]的文章
[Yu, Albert C. H.]的文章
[He, Qi Hua]的文章
百度学术
百度学术中相似的文章
[Wang, Ai Ling]的文章
[Yu, Albert C. H.]的文章
[He, Qi Hua]的文章
必应学术
必应学术中相似的文章
[Wang, Ai Ling]的文章
[Yu, Albert C. H.]的文章
[He, Qi Hua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。