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学科主题: 临床医学
题名:
AGEs mediated expression and secretion of TNF alpha in rail retinal microglia
作者: Wang, Ai Ling; Yu, Albert C. H.; He, Qi Hua; Zhu, Xiu′ An; Tso, Mark O. M.
关键词: AGEs ; retinal microglia ; diabetic retinopathy ; TNF alpha
刊名: EXPERIMENTAL EYE RESEARCH
发表日期: 2007-05-01
DOI: 10.1016/j.exer.2007.01.011
卷: 84, 期:5, 页:905-913
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: GLYCATION END-PRODUCTS ; NITRIC-OXIDE SYNTHASE ; KAPPA-B ACTIVATION ; DIABETIC-RETINOPATHY ; GENE-EXPRESSION ; ALVEOLAR MACROPHAGES ; PARKINSONS-DISEASE ; ENDOTHELIAL-CELLS ; LIPOPOLYSACCHARIDE ; ENDPRODUCTS
英文摘要:

Diabetic retinopathy induces an inflammatory response in the retina characterized by an increase in inflammatory cytokines and the activation of microglia. The degree of microglia activation may influence the extent of retina injury following retinal metabolic stress. We have previously shown that DR rats have elevated levels of advanced glycation end products (AGEs) in their blood. We have also suggested that AGEs might be involved in microglial activation and production of tumor necrosis factor alpha (TNF alpha). In this study, we attempted to confirm that AGEs induce the release of TNF alpha from rat retinal microglia using an in vitro microglia culture system, and concurrently to explore the mediating mechanisms. AGEs increased the protein secretion and mRNA expression of TNF alpha in cultured rat retinal microglia. These effects of AGEs were primarily mediated by reactive oxygen species (ROS). Furthermore, the inhibitors for mitogen-activated protein kinases (MAPK; p38, JNK and ERK 1/2) and nuclear factor-kB (NF-kB) could significantly decrease AGEs-induced TNF alpha release. AGEs-activated microglia showed an increase of NFkB p65 nuclear translocation. These observations indicated that pathophysiological levels of AGEs may alter rat retinal microglia function by upregulating TNF a expression and release via enhanced formation of intracellular ROS. AGEs-induced ROS subsequently activates MAPK (P38, JNK and ERK1/2) and NF-kB. (C) 2007 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000246534000013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55593
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Eye Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Key Lab Neurosci, PKU, Neurosci Res Inst, Beijing 100083, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Minist Educ, Beijing 100083, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Beijing 100083, Peoples R China
6.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21218 USA

Recommended Citation:
Wang, Ai Ling,Yu, Albert C. H.,He, Qi Hua,et al. AGEs mediated expression and secretion of TNF alpha in rail retinal microglia[J]. EXPERIMENTAL EYE RESEARCH,2007,84(5):905-913.
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