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学科主题: 临床医学
题名:
Chromatography studies on bio-affinity of nine ligands of alpha(1)-adrenoceptor to alpha(1D) subtypes overexpressed in cell membrane
作者: Zhang, DA; Yuan, BX; Deng, XL; Yang, GD; He, LC; Zhang, YY; Han, QD
关键词: cell membrane ; chromatography ; alpha(1)-adrenergic receptor ; alpha(1D)-adrenoceptor subtype
刊名: SCIENCE IN CHINA SERIES C-LIFE SCIENCES
发表日期: 2004-08-01
DOI: 10.1360/03yc0109
卷: 47, 期:4, 页:376-381
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: ALPHA-1-ADRENERGIC RECEPTOR ; SELECTIVE ANTAGONISTS ; MOLECULAR-CLONING ; SILICA SURFACE ; EXPRESSION ; CDNA
英文摘要:

To improve selectivity and specificity of cell membrane chromatography (CMC), the chromatography affinities of nine ligands of alpha(1)-adrenergic receptor(AR)to alpha(1D)-AR subtype were investigated. The human embryonic kidney (HEK) 293 cells expressed by cDNA of a1D-AR subtypes were cultured and cell membrane stationary phase (CMSP) was prepared. Then the interactions between ligands and a1D-AR in CMSP were investigated using CMC. The affinity rank order to alpha(1D)-AR subtype obtained from CMC for the nine alpha(1)-adrenoceceptor ligands is: prazosin, BMY7378, phentolamine, oxymetazoline, 5-methylurapidil, norepinephrine, phenylephrine, methoxamine, RS-17053. The affinity rank order is similar and correlates well with that obtained from others′ radioligand binding assays (RBA). CMSP prepared by transfected HEK293 cells with alpha(1D)-adrenoceptor cDNA and CMC method could be used to evaluate affinities of drug-receptor and drug-receptor subtypes and to screen drugs selective to a1D-AR.

语种: 英语
WOS记录号: WOS:000224138900010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55604
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Beijing Univ, Hosp 3, Beijing 100083, Peoples R China
2.Xian Jiaotong Univ, Sch Med, Xian 710061, Peoples R China
3.Xian Jiaotong Univ, Coll Pharm, Xian 710061, Peoples R China

Recommended Citation:
Zhang, DA,Yuan, BX,Deng, XL,et al. Chromatography studies on bio-affinity of nine ligands of alpha(1)-adrenoceptor to alpha(1D) subtypes overexpressed in cell membrane[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2004,47(4):376-381.
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