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Chromatography studies on bio-affinity of nine ligands of alpha(1)-adrenoceptor to alpha(1D) subtypes overexpressed in cell membrane
Zhang, DA; Yuan, BX; Deng, XL; Yang, GD; He, LC; Zhang, YY; Han, QD
关键词Cell Membrane Chromatography Alpha(1)-adrenergic Receptor Alpha(1d)-adrenoceptor Subtype
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2004-08-01
DOI10.1360/03yc0109
47期:4页:376-381
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]ALPHA-1-ADRENERGIC RECEPTOR ; SELECTIVE ANTAGONISTS ; MOLECULAR-CLONING ; SILICA SURFACE ; EXPRESSION ; CDNA
英文摘要

To improve selectivity and specificity of cell membrane chromatography (CMC), the chromatography affinities of nine ligands of alpha(1)-adrenergic receptor(AR)to alpha(1D)-AR subtype were investigated. The human embryonic kidney (HEK) 293 cells expressed by cDNA of a1D-AR subtypes were cultured and cell membrane stationary phase (CMSP) was prepared. Then the interactions between ligands and a1D-AR in CMSP were investigated using CMC. The affinity rank order to alpha(1D)-AR subtype obtained from CMC for the nine alpha(1)-adrenoceceptor ligands is: prazosin, BMY7378, phentolamine, oxymetazoline, 5-methylurapidil, norepinephrine, phenylephrine, methoxamine, RS-17053. The affinity rank order is similar and correlates well with that obtained from others′ radioligand binding assays (RBA). CMSP prepared by transfected HEK293 cells with alpha(1D)-adrenoceptor cDNA and CMC method could be used to evaluate affinities of drug-receptor and drug-receptor subtypes and to screen drugs selective to a1D-AR.

语种英语
WOS记录号WOS:000224138900010
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55604
专题北京大学第三临床医学院
作者单位1.Beijing Univ, Hosp 3, Beijing 100083, Peoples R China
2.Xian Jiaotong Univ, Sch Med, Xian 710061, Peoples R China
3.Xian Jiaotong Univ, Coll Pharm, Xian 710061, Peoples R China
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GB/T 7714
Zhang, DA,Yuan, BX,Deng, XL,et al. Chromatography studies on bio-affinity of nine ligands of alpha(1)-adrenoceptor to alpha(1D) subtypes overexpressed in cell membrane[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2004,47(4):376-381.
APA Zhang, DA.,Yuan, BX.,Deng, XL.,Yang, GD.,He, LC.,...&Han, QD.(2004).Chromatography studies on bio-affinity of nine ligands of alpha(1)-adrenoceptor to alpha(1D) subtypes overexpressed in cell membrane.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,47(4),376-381.
MLA Zhang, DA,et al."Chromatography studies on bio-affinity of nine ligands of alpha(1)-adrenoceptor to alpha(1D) subtypes overexpressed in cell membrane".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 47.4(2004):376-381.
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