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学科主题: 临床医学
题名:
Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1
作者: Xie, Wei; Jiang, Peng; Miao, Lin; Zhao, Ying; Zhimin, Zhai; Qing, Li; Zhu, Wei-guo; Wu, Mian
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2006
DOI: 10.1093/nar/gkl150
卷: 34, 期:7, 页:2046-2055
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: COLON-CANCER CELLS ; E2F-1-INDUCED APOPTOSIS ; P53-DEPENDENT APOPTOSIS ; E2F1-INDUCED APOPTOSIS ; MITOCHONDRIAL PROTEIN ; C-MYC ; TARGET ; EXPRESSION ; P53 ; ARF
英文摘要:

Deregulated expression of E2F1 not only promotes S-phase entry but also induces apoptosis. Although it has been well documented that E2F1 is able to induce p53-dependent apoptosis via raising ARF activity, the mechanism by which E2F induces p53-independent apoptosis remains unclear. Here we report that E2F1 can directly bind to and activate the promoter of Smac/DIABLO, a mitochondrial proapoptotic gene, through the E2F1-binding sites BS2 (-542 similar to -535 bp) and BS3 (-200 similar to -193 bp). BS2 and BS3 appear to be utilized in combination rather than singly by E2F1 in activation of Smac/DIABLO. Activation of BS2 and BS3 are E2F1-specific, since neither E2F2 nor E2F3 is able to activate BS2 or BS3. Using the H1299 ER-E2F1 cell line where E2F1 activity can be conditionally induced, E2F1 has been shown to upregulate the Smac/DIABLO expression at both mRNA and protein levels upon 4-hydroxytamoxifen treatment, resulting in an enhanced mitochondria-mediated apoptosis. Reversely, reducing the Smac/DIABLO expression by RNA interference significantly diminishes apoptosis induced by E2F1. These results may suggest a novel mechanism by which E2F1 promotes p53-independent apoptosis through directly regulating its downstream mitochondrial apoptosis-inducing factors, such as Smac/DIABLO.

语种: 英语
WOS记录号: WOS:000237522900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55615
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Anhui Prov Hosp, Cent Lab, Hefei 230001, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Canc Res Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Xie, Wei,Jiang, Peng,Miao, Lin,et al. Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1[J]. NUCLEIC ACIDS RESEARCH,2006,34(7):2046-2055.
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