IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1
Xie, Wei; Jiang, Peng; Miao, Lin; Zhao, Ying; Zhimin, Zhai; Qing, Li; Zhu, Wei-guo; Wu, Mian
刊名NUCLEIC ACIDS RESEARCH
2006
DOI10.1093/nar/gkl150
34期:7页:2046-2055
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]COLON-CANCER CELLS ; E2F-1-INDUCED APOPTOSIS ; P53-DEPENDENT APOPTOSIS ; E2F1-INDUCED APOPTOSIS ; MITOCHONDRIAL PROTEIN ; C-MYC ; TARGET ; EXPRESSION ; P53 ; ARF
英文摘要

Deregulated expression of E2F1 not only promotes S-phase entry but also induces apoptosis. Although it has been well documented that E2F1 is able to induce p53-dependent apoptosis via raising ARF activity, the mechanism by which E2F induces p53-independent apoptosis remains unclear. Here we report that E2F1 can directly bind to and activate the promoter of Smac/DIABLO, a mitochondrial proapoptotic gene, through the E2F1-binding sites BS2 (-542 similar to -535 bp) and BS3 (-200 similar to -193 bp). BS2 and BS3 appear to be utilized in combination rather than singly by E2F1 in activation of Smac/DIABLO. Activation of BS2 and BS3 are E2F1-specific, since neither E2F2 nor E2F3 is able to activate BS2 or BS3. Using the H1299 ER-E2F1 cell line where E2F1 activity can be conditionally induced, E2F1 has been shown to upregulate the Smac/DIABLO expression at both mRNA and protein levels upon 4-hydroxytamoxifen treatment, resulting in an enhanced mitochondria-mediated apoptosis. Reversely, reducing the Smac/DIABLO expression by RNA interference significantly diminishes apoptosis induced by E2F1. These results may suggest a novel mechanism by which E2F1 promotes p53-independent apoptosis through directly regulating its downstream mitochondrial apoptosis-inducing factors, such as Smac/DIABLO.

语种英语
WOS记录号WOS:000237522900015
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被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55615
专题北京大学临床肿瘤学院
作者单位1.Anhui Prov Hosp, Cent Lab, Hefei 230001, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
3.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Canc Res Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Xie, Wei,Jiang, Peng,Miao, Lin,et al. Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1[J]. NUCLEIC ACIDS RESEARCH,2006,34(7):2046-2055.
APA Xie, Wei.,Jiang, Peng.,Miao, Lin.,Zhao, Ying.,Zhimin, Zhai.,...&Wu, Mian.(2006).Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1.NUCLEIC ACIDS RESEARCH,34(7),2046-2055.
MLA Xie, Wei,et al."Novel link between E2F1 and Smac/DIABLO: proapoptotic Smac/DIABLO is transcriptionally upregulated by E2F1".NUCLEIC ACIDS RESEARCH 34.7(2006):2046-2055.
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