北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Autophagy activation attenuates renal ischemia-reperfusion injury in rats
作者: Zhang, Ya-Li; Zhang, Jie; Cui, Li-Yan; Yang, Shuo
关键词: Autophagy ; ischemia reperfusion ; apoptosis ; acute kidney injury
刊名: EXPERIMENTAL BIOLOGY AND MEDICINE
发表日期: 2015-12-01
DOI: 10.1177/1535370215581306
卷: 240, 期:12, 页:1590-1598
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: ACUTE KIDNEY INJURY ; TUBULAR APOPTOSIS ; CELL-DEATH ; FAILURE ; ISCHEMIA/REPERFUSION ; MECHANISMS ; PROTECTS ; INHIBITION ; CASPASE-3 ; RAPAMYCIN
英文摘要:

Ischemia-reperfusion (I/R) injury is a leading cause of acute kidney injury (AKI), which is a common clinical complication but lacks effective therapies. This study investigated the role of autophagy in renal I/R injury and explored potential mechanisms in an established rat renal I/R injury model. Forty male Wistar rats were randomly divided into four groups: Sham, I/R, I/R pretreated with 3-methyladenine (3-MA, autophagy inhibitor), or I/R pretreated with rapamycin (autophagy activator). All rats were subjected to clamping of the left renal pedicle for 45min after right nephrectomy, followed by 24h of reperfusion. The Sham group underwent the surgical procedure without ischemia. 3-MA and rapamycin were injected 15min before ischemia. Renal function was indicated by blood urea nitrogen and serum creatinine. Tissue samples from the kidneys were scored histopathologically. Autophagy was indicated by light chain 3 (LC3), Beclin-1, and p62 levels and the number of autophagic vacuoles. Apoptosis was evaluated by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and expression of caspase-3. Autophagy was activated after renal I/R injury. Inhibition of autophagy by 3-MA before I/R aggravated renal injury, with worsened renal function, higher renal tissue injury scores, and more tubular apoptosis. In contrast, rapamycin pretreatment ameliorated renal injury, with improved renal function, lower renal tissue injury scores, and inhibited apoptosis based on fewer TUNEL-positive cells and lower caspase-3 expression. Our results demonstrate that autophagy could be activated during I/R injury and play a protective role in renal I/R injury. The mechanisms were involved in the regulation of several autophagy and apoptosis-related genes. Furthermore, autophagy activator may be a promising therapy for I/R injury and AKI in the future.

语种: 英语
所属项目编号: 81271903 ; 2012BA137B01
项目资助者: Natural Science Foundation of China ; National Science and Technology Ministry
WOS记录号: WOS:000366000500005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55627
Appears in Collections:北京大学第三临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Dept Lab Med, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Ya-Li,Zhang, Jie,Cui, Li-Yan,et al. Autophagy activation attenuates renal ischemia-reperfusion injury in rats[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2015,240(12):1590-1598.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Ya-Li]'s Articles
[Zhang, Jie]'s Articles
[Cui, Li-Yan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Ya-Li]‘s Articles
[Zhang, Jie]‘s Articles
[Cui, Li-Yan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace