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Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes
Chen, Xue1,2; Takai, Toshiro1; Xie, Yang1,3; Niyonsaba, Francois1; Okumura, Ko1; Ogawa, Hideoki1
关键词Keratinocyte Proinflammatory Response Ll-37 Th17 Cytokines Tnf-alpha Ifn-gamma Double-stranded Rna
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2013-04-19
DOI10.1016/j.bbrc.2013.03.024
433期:4页:532-537
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]HOST-DEFENSE PEPTIDES ; HUMAN DENDRITIC CELLS ; CATHELICIDIN PEPTIDES ; ATOPIC-DERMATITIS ; EPITHELIAL-CELLS ; BETA-DEFENSINS ; TSLP ; SKIN ; INFLAMMATION ; EXPRESSION
英文摘要

Epidermal keratinocytes produce proinflammatory cytokines/chemokines upon stimulation with cytokine milieus and Toll-like receptor ligands, which are considered to reflect epidermal environments in inflamed skin. The human antimicrobial peptide LL-37, besides having microbicidal functions, plays multiple roles as a "host defense peptide" in the immune system. Here, we examined the effect of LL-37 on proinflammatory responses induced by double-stranded RNA (dsRNA) and cytokines in primary human keratinocytes. LL-37 inhibited dsRNA-induced production of thymic stromal lymphopoietin (TSLP), CCL5/RANTES, CXCL10/IP-10, and CXCL8/1-8, which was attributable to interaction between LL-37 and dsRNA, although LL-37 upregulated CXCL8 expression at an earlier time point (8 h). LL-37 inhibited the increase of CXCL10 and CCL5 induced by TNF-alpha- and/or IFN-gamma but enhanced that of CXCL8. LL-37 and Th17 cytokines (IL-17 and IL-22) synergistically upregulated the expression of CXCL8 and IL-6. LL-37 showed the effects above at a high concentration (25 mu g/ml, 5.6 mu M). We also examined effects of a peptide with a scrambled LL-37 sequence, which has been frequently used as a negative control, and those of another peptide with the reversed LL-37 sequence, activities of which have not been well investigated. Interestingly, the reversed LL-37 had effects similar to LL-37 but the scrambled LL-37 did not. The modulation by LL-37 of the keratinocyte proinflammatory responses induced by cytokine milieus and dsRNA suggests novel roles for LL-37 in skin inflammation such as the promotion of IL17/IL-22/IL-6-associated psoriasis and suppression of TSLP-associated atopic dermatitis. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000318259100030
资助机构Ministry of Education, Culture, Sports, Science and Technology (MEXT) ; MEXT
引用统计
被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55631
专题北京大学第二临床医学院_皮科
作者单位1.Juntendo Univ, Grad Sch Med, Atopy Allergy Res Ctr, Tokyo 1138421, Japan
2.Peking Univ, Peoples Hosp, Dept Dermatol, Beijing 100044, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Dermatol, Guangzhou 510630, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Chen, Xue,Takai, Toshiro,Xie, Yang,et al. Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2013,433(4):532-537.
APA Chen, Xue,Takai, Toshiro,Xie, Yang,Niyonsaba, Francois,Okumura, Ko,&Ogawa, Hideoki.(2013).Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,433(4),532-537.
MLA Chen, Xue,et al."Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 433.4(2013):532-537.
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