北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病理学系  > 期刊论文
学科主题: 基础医学
题名:
Screening for EGFR and KRAS mutations in non-small cell lung carcinomas using DNA extraction by hydrothermal pressure coupled with PCR-based direct sequencing
作者: Liu, Yan1; Wu, Bing-Quan1; Zhong, Hao-Hao1; Hui, Pei2; Fang, Wei-Gang1
关键词: EGFR ; KRAS ; FFPE ; hydrothermal pressure ; lung cancer ; mutation analysis
刊名: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
发表日期: 2013
卷: 6, 期:9, 页:1880-1889
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Pathology
研究领域[WOS]: Oncology ; Pathology
关键词[WOS]: GROWTH-FACTOR-RECEPTOR ; TYROSINE KINASE INHIBITORS ; TRANSBRONCHIAL NEEDLE ASPIRATION ; CANCER ; BIOPSY ; ADENOCARCINOMAS ; RESISTANCE ; GEFITINIB ; FREQUENT ; SAMPLES
英文摘要:

EGFR and KRAS mutations correlate with response to tyrosine kinase inhibitors in patients with non-small cell lung carcinoma (NSCLC). We reported a hydrothermal pressure method of simultaneous deparaffinization and lysis of formalin-fixed paraffin embedded (FFPE) tissue followed by conventional chaotropic salt column purification to obtain high quality DNA for mutation analysis using PCR-base direct sequencing. This study assessed the feasibility of using this method to screen for exons 18-21 of EGFR and exon 2 of KRAS gene mutations in surgical resection and core needle biopsy specimens from 251 NSCLC patients. EGFR mutations were identified in 140 (55.8%) NSCLC patients (118 in adenocarcinoma, 11 in squamous cell carcinoma, 7 in adenocarcinoma and 4 in NSCLC-not otherwise specified), including four novel substitutions (L718M, A743V, L815P, V819E). EGFR mutations were frequently present in female patients (72 of 113, 63.7%) and NSCLC with adenocarcinoma component (125/204, 61.3%) with statistical significance. Twenty-one patients had multiple mutations at different exons of EGFR, in which seventeen patients had deletions in exon 19. KRAS mutations were found in 18 (7.2%) patients (15 in adenocarcinoma, 2 in squamous cell carcinoma and one in NSCLC-not otherwise specified), including an uncommon substitution G13C. Deparaffinization and lysis by hydrothermal pressure, coupled with purification and PCR-based sequencing, provides a robust screening approach for EGFR and KRAS mutation analysis of FFPE tissues from either surgical resection or core needle biopsy in clinical personalized management of lung cancer.

语种: 英语
WOS记录号: WOS:000324316600020
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55634
Appears in Collections:基础医学院_病理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Screening for EGFR and KRAS mutations in non-small cell lung carcinomas using DNA extraction by hydrothermal pressure coupled with PCR-based direct sequencing.pdf(444KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Dept Pathol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06510 USA

Recommended Citation:
Liu, Yan,Wu, Bing-Quan,Zhong, Hao-Hao,et al. Screening for EGFR and KRAS mutations in non-small cell lung carcinomas using DNA extraction by hydrothermal pressure coupled with PCR-based direct sequencing[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2013,6(9):1880-1889.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Yan]'s Articles
[Wu, Bing-Quan]'s Articles
[Zhong, Hao-Hao]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Yan]‘s Articles
[Wu, Bing-Quan]‘s Articles
[Zhong, Hao-Hao]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace