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学科主题: 基础医学
题名:
RNF 122: A novel ubiquitin ligase associated with calcium-modulating cyclophilin ligand
作者: Peng, Zhi1,4; Shi, Taiping1,2,3; Ma, Dalong1,2,3
刊名: BMC CELL BIOLOGY
发表日期: 2010-06-17
DOI: 10.1186/1471-2121-11-41
卷: 11, 期:0
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: PROTEASOME SYSTEM ; FACTOR RECEPTOR ; CELL-GROWTH ; PROTEIN ; CAML ; ACTIVATION
英文摘要:

Background: RNF122 is a recently discovered RING finger protein that is associated with HEK293T cell viability and is overexpressed in anaplastic thyroid cancer cells. RNF122 owns a RING finger domain in C terminus and transmembrane domain in N terminus. However, the biological mechanism underlying RNF122 action remains unknown.

Results: In this study, we characterized RNF122 both biochemically and intracellularly in order to gain an understanding of its biological role. RNF122 was identified as a new ubiquitin ligase that can ubiquitinate itself and undergoes degradation in a RING finger-dependent manner. From a yeast two-hybrid screen, we identified calciummodulating cyclophilin ligand (CAML) as an RNF122-interacting protein. To examine the interaction between CAML and RNF122, we performed co-immunoprecipitation and colocalization experiments using intact cells. What is more, we found that CAML is not a substrate of ubiquitin ligase RNF122, but that, instead, it stabilizes RNF122.

Conclusions: RNF122 can be characterized as a C3H2C3-type RING finger-containing E3 ubiquitin ligase localized to the ER. RNF122 promotes its own degradation in a RING finger-and proteasome-dependent manner. RNF122 interacts with CAML, and its E3 ubiquitin ligase activity was noted to be dependent on the RING finger domain.

语种: 英语
所属项目编号: 2006AA02A305 ; 30600547 ; 2009ZX09503-004
项目资助者: National High-tech R& ; D Program (863 Program) of China ; National Natural Science Foundation of China ; key national S& ; T Program-"Major New Drug Development"
WOS记录号: WOS:000280364100001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55650
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
4.Yijishan Hosp, Wannan Med Coll, Dept Med Oncol, Wuhu 241001, Anhui, Peoples R China

Recommended Citation:
Peng, Zhi,Shi, Taiping,Ma, Dalong. RNF 122: A novel ubiquitin ligase associated with calcium-modulating cyclophilin ligand[J]. BMC CELL BIOLOGY,2010,11(0).
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