北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
RNF 122: A novel ubiquitin ligase associated with calcium-modulating cyclophilin ligand
作者: Peng, Zhi1,4; Shi, Taiping1,2,3; Ma, Dalong1,2,3
刊名: BMC CELL BIOLOGY
发表日期: 2010-06-17
DOI: 10.1186/1471-2121-11-41
卷: 11, 期:0
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: PROTEASOME SYSTEM ; FACTOR RECEPTOR ; CELL-GROWTH ; PROTEIN ; CAML ; ACTIVATION
英文摘要:

Background: RNF122 is a recently discovered RING finger protein that is associated with HEK293T cell viability and is overexpressed in anaplastic thyroid cancer cells. RNF122 owns a RING finger domain in C terminus and transmembrane domain in N terminus. However, the biological mechanism underlying RNF122 action remains unknown.

Results: In this study, we characterized RNF122 both biochemically and intracellularly in order to gain an understanding of its biological role. RNF122 was identified as a new ubiquitin ligase that can ubiquitinate itself and undergoes degradation in a RING finger-dependent manner. From a yeast two-hybrid screen, we identified calciummodulating cyclophilin ligand (CAML) as an RNF122-interacting protein. To examine the interaction between CAML and RNF122, we performed co-immunoprecipitation and colocalization experiments using intact cells. What is more, we found that CAML is not a substrate of ubiquitin ligase RNF122, but that, instead, it stabilizes RNF122.

Conclusions: RNF122 can be characterized as a C3H2C3-type RING finger-containing E3 ubiquitin ligase localized to the ER. RNF122 promotes its own degradation in a RING finger-and proteasome-dependent manner. RNF122 interacts with CAML, and its E3 ubiquitin ligase activity was noted to be dependent on the RING finger domain.

语种: 英语
所属项目编号: 2006AA02A305 ; 30600547 ; 2009ZX09503-004
项目资助者: National High-tech R& ; D Program (863 Program) of China ; National Natural Science Foundation of China ; key national S& ; T Program-"Major New Drug Development"
WOS记录号: WOS:000280364100001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55650
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1471-2121-11-41.pdf(680KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100191, Peoples R China
4.Yijishan Hosp, Wannan Med Coll, Dept Med Oncol, Wuhu 241001, Anhui, Peoples R China

Recommended Citation:
Peng, Zhi,Shi, Taiping,Ma, Dalong. RNF 122: A novel ubiquitin ligase associated with calcium-modulating cyclophilin ligand[J]. BMC CELL BIOLOGY,2010,11(0).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Peng, Zhi]'s Articles
[Shi, Taiping]'s Articles
[Ma, Dalong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Peng, Zhi]‘s Articles
[Shi, Taiping]‘s Articles
[Ma, Dalong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace