|Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer|
|Song, Ming-yang1; Pan, Kai-feng1; Su, Hui-juan1; Zhang, Lian1; Ma, Jun-ling1; Li, Ji-you2; Yuasa, Yasuhito3; Kang, Daehee4; Kim, Yong Sung5; You, Wei-cheng1|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Science & Technology - Other Topics|
|关键词[WOS]||HIGH-RISK ; CLINICOPATHOLOGICAL FEATURES ; CIRCULATING MICRORNAS ; EXPRESSION ; LESIONS ; POPULATION ; MIR-221 ; PLASMA ; ADENOCARCINOMA ; SURVIVAL|
Background: To investigate the potential of serum miRNAs as biomarkers for early detection of gastric cancer (GC), a population-based study was conducted in Linqu, a high-risk area of GC in China.
Methodology/Principal Findings: All subjects were selected from two large cohort studies. Differential miRNAs were identified in serum pools of GC and control using TaqMan low density array, and validated in individual from 82 pairs of GC and control, and 46 pairs of dysplasia and control by real-time quantitative reverse transcription-polymerase chain reaction. The temporal trends of identified serum miRNA expression were further explored in a retrospective study on 58 GC patients who had at least one pre-GC diagnosis serum sample based on the long-term follow-up population. The miRNA profiling results demonstrated that 16 miRNAs were markedly upregulated in GC patients compared to controls. Further validation identified a panel of three serum miRNAs (miR-221, miR-744, and miR-376c) as potential biomarkers for GC detection, and receiver operating characteristic (ROC) curve-based risk assessment analysis revealed that this panel could distinguish GCs from controls with 82.4% sensitivity and 58.8% specificity. MiR-221 and miR-376c demonstrated significantly positive correlation with poor differentiation of GC, and miR-221 displayed higher level in dysplasia than in control. Furthermore, the retrospective study revealed an increasing trend of these three miRNA levels during GC development (P for trend, 0.05), and this panel could classify serum samples collected up to 5 years ahead of clinical GC diagnosis with 79.3% overall accuracy.
Conclusions/Significance: These data suggest that serum miR-221, miR-376c and miR-744 have strong potential as novel non-invasive biomarkers for early detection of GC.
|项目编号||2010CB529303 ; 30921140311 ; 81041012 ; 81171989|
|资助机构||National Basic Research Program of China (973 Program) ; Natural Science Foundation of China ; National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Canc Hosp & Inst, Dept Canc Epidemiol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China|
2.Peking Univ, Canc Hosp & Inst, Dept Pathol, Beijing 100871, Peoples R China
3.Tokyo Med & Dent Univ, Dept Mol Oncol, Tokyo, Japan
4.Seoul Natl Univ, Coll Med, Dept Prevent Med, Seoul, South Korea
5.Med Genom Res Ctr, Korea Res Inst Biosci & Biotechnol, Taejon, South Korea
|Song, Ming-yang,Pan, Kai-feng,Su, Hui-juan,et al. Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer[J]. PLOS ONE,2012,7(3).|
|APA||Song, Ming-yang.,Pan, Kai-feng.,Su, Hui-juan.,Zhang, Lian.,Ma, Jun-ling.,...&You, Wei-cheng.(2012).Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer.PLOS ONE,7(3).|
|MLA||Song, Ming-yang,et al."Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer".PLOS ONE 7.3(2012).|