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IR@PKUHSC  > 北京大学临床肿瘤学院  > 流行病学研究室  > 期刊论文
学科主题: 临床医学
题名:
Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer
作者: Song, Ming-yang1; Pan, Kai-feng1; Su, Hui-juan1; Zhang, Lian1; Ma, Jun-ling1; Li, Ji-you2; Yuasa, Yasuhito3; Kang, Daehee4; Kim, Yong Sung5; You, Wei-cheng1
刊名: PLOS ONE
发表日期: 2012-03-14
DOI: 10.1371/journal.pone.0033608
卷: 7, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: HIGH-RISK ; CLINICOPATHOLOGICAL FEATURES ; CIRCULATING MICRORNAS ; EXPRESSION ; LESIONS ; POPULATION ; MIR-221 ; PLASMA ; ADENOCARCINOMA ; SURVIVAL
英文摘要:

Background: To investigate the potential of serum miRNAs as biomarkers for early detection of gastric cancer (GC), a population-based study was conducted in Linqu, a high-risk area of GC in China.

Methodology/Principal Findings: All subjects were selected from two large cohort studies. Differential miRNAs were identified in serum pools of GC and control using TaqMan low density array, and validated in individual from 82 pairs of GC and control, and 46 pairs of dysplasia and control by real-time quantitative reverse transcription-polymerase chain reaction. The temporal trends of identified serum miRNA expression were further explored in a retrospective study on 58 GC patients who had at least one pre-GC diagnosis serum sample based on the long-term follow-up population. The miRNA profiling results demonstrated that 16 miRNAs were markedly upregulated in GC patients compared to controls. Further validation identified a panel of three serum miRNAs (miR-221, miR-744, and miR-376c) as potential biomarkers for GC detection, and receiver operating characteristic (ROC) curve-based risk assessment analysis revealed that this panel could distinguish GCs from controls with 82.4% sensitivity and 58.8% specificity. MiR-221 and miR-376c demonstrated significantly positive correlation with poor differentiation of GC, and miR-221 displayed higher level in dysplasia than in control. Furthermore, the retrospective study revealed an increasing trend of these three miRNA levels during GC development (P for trend, 0.05), and this panel could classify serum samples collected up to 5 years ahead of clinical GC diagnosis with 79.3% overall accuracy.

Conclusions/Significance: These data suggest that serum miR-221, miR-376c and miR-744 have strong potential as novel non-invasive biomarkers for early detection of GC.

语种: 英语
所属项目编号: 2010CB529303 ; 30921140311 ; 81041012 ; 81171989
项目资助者: National Basic Research Program of China (973 Program) ; Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号: WOS:000303198600079
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55706
Appears in Collections:北京大学临床肿瘤学院_流行病学研究室_期刊论文

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作者单位: 1.Peking Univ, Canc Hosp & Inst, Dept Canc Epidemiol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Canc Hosp & Inst, Dept Pathol, Beijing 100871, Peoples R China
3.Tokyo Med & Dent Univ, Dept Mol Oncol, Tokyo, Japan
4.Seoul Natl Univ, Coll Med, Dept Prevent Med, Seoul, South Korea
5.Med Genom Res Ctr, Korea Res Inst Biosci & Biotechnol, Taejon, South Korea

Recommended Citation:
Song, Ming-yang,Pan, Kai-feng,Su, Hui-juan,et al. Identification of Serum MicroRNAs as Novel Non-Invasive Biomarkers for Early Detection of Gastric Cancer[J]. PLOS ONE,2012,7(3).
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