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学科主题: 基础医学
题名:
Role of berberine in anti-bacterial as a high-affinity LPS antagonist binding to TLR4/MD-2 receptor
作者: Chu, Ming1; Ding, Ran2; Chu, Zheng-yun2; Zhang, Ming-bo2; Liu, Xiao-yan1; Xie, Shao-hua1; Zhai, Yan-jun2; Wang, Yue-dan1
刊名: BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
发表日期: 2014-03-06
DOI: 10.1186/1472-6882-14-89
卷: 14, 期:6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Integrative & Complementary Medicine
研究领域[WOS]: Integrative & Complementary Medicine
关键词[WOS]: NEUTRAL SULFATE BERBERINE ; LIPOPOLYSACCHARIDE LPS ; INTESTINAL INJURY ; STRUCTURAL BASIS ; HIGH-THROUGHPUT ; TNF-ALPHA ; PROTEIN ; TLR4 ; SECRETION ; ENDOTOXIN
英文摘要:

Background: Berberine is an isoquinoline alkaloid mainly extracted from Rhizoma Coptidis and has been shown to possess a potent inhibitory activity against bacterial. However, the role of berberine in anti-bacterial action has not been extensively studied.

Methods: The animal model was established to investigate the effects of berberine on bacterial and LPS infection. Docking analysis, Molecular dynamics simulations and Real-time RT-PCR analysis was adopted to investigate the molecular mechanism.

Results: Treatment with 40 mg/kg berberine significantly increased the survival rate of mice challenged with Salmonella typhimurium (LT2), but berberine show no effects in bacteriostasis. Further study indicated that treatment with 0.20 g/kg berberine markedly increased the survival rate of mice challenged with 2 EU/ml bacterial endotoxin (LPS) and postpone the death time of the dead mice. Moreover, pretreatment with 0.05 g/kg berberine significantly lower the increasing temperature of rabbits challenged with LPS. The studies of molecular mechanism demonstrated that Berberine was able to bind to the TLR4/MD-2 receptor, and presented higher affinity in comparison with LPS. Furthermore, berberine could significantly suppressed the increasing expression of NF-kappa B, IL-6, TNF alpha, and IFN beta in the RAW264.7 challenged with LPS.

Conclusion: Berberine can act as a LPS antagonist and block the LPS/TLR4 signaling from the sourse, resulting in the anti-bacterial action.

语种: 英语
所属项目编号: 81172884 ; J1030831/J0108
项目资助者: National Natural Science Foundation of China ; Fund for Fostering Talents in Basic Science of the National Natural Science Foundation of China
WOS记录号: WOS:000332557700001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55709
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Immunol, Beijing 100191, Peoples R China
2.Liao Ning Univ Tradit Chinese Med, Dept Pharm, Liaoning 116600, Peoples R China

Recommended Citation:
Chu, Ming,Ding, Ran,Chu, Zheng-yun,et al. Role of berberine in anti-bacterial as a high-affinity LPS antagonist binding to TLR4/MD-2 receptor[J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE,2014,14(6).
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