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学科主题口腔医学
Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering
Gao, Xiang1,2; Zhang, Xiaohong3; Song, Jinlin1,2; Xu, Xiao4; Xu, Anxiu1; Wang, Mengke4; Xie, Bingwu1; Huang, Enyi2; Deng, Feng1,2; Wei, Shicheng2,3,4
关键词Biomimetic Nanofiber Peptide Bone Tissue Engineering
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
2015
DOI10.2147/IJN.S94045
10页:7109-7128
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]MESENCHYMAL STEM-CELLS ; OSTEOGENIC DIFFERENTIATION ; ELECTROSPUN FIBERS ; STROMAL CELLS ; NANOTOPOGRAPHY ; IMMOBILIZATION ; REGENERATION ; COMMITMENT ; BMP-2
英文摘要

The construction of functional biomimetic scaffolds that recapitulate the topographical and biochemical features of bone tissue extracellular matrix is now of topical interest in bone tissue engineering. In this study, a novel surface-functionalized electrospun polycaprolactone (PCL) nanofiber scaffold with highly ordered structure was developed to simulate the critical features of native bone tissue via a single step of catechol chemistry. Specially, under slightly alkaline aqueous solution, polydopamine (pDA) was coated on the surface of aligned PCL nanofibers after electrospinning, followed by covalent immobilization of bone morphogenetic protein-7-derived peptides onto the pDA-coated nanofiber surface. Contact angle measurement, Raman spectroscopy, and X-ray photoelectron spectroscopy confirmed the presence of pDA and peptides on PCL nanofiber surface. Our results demonstrated that surface modification with osteoinductive peptides could improve cytocompatibility of nanofibers in terms of cell adhesion, spreading, and proliferation. Most importantly, Alizarin Red S staining, quantitative real-time polymerase chain reaction, immunostaining, and Western blot revealed that human mesenchymal stem cells cultured on aligned nanofibers with osteoinductive peptides exhibited enhanced osteogenic differentiation potential than cells on randomly oriented nanofibers. Furthermore, the aligned nanofibers with osteoinductive peptides could direct osteogenic differentiation of human mesenchymal stem cells even in the absence of osteoinducting factors, suggesting superior osteogenic efficacy of biomimetic design that combines the advantages of osteoinductive peptide signal and highly ordered nanofibers on cell fate decision. The presented peptide-decorated bone-mimic nanofiber scaffolds hold a promising potential in the context of bone tissue engineering.

语种英语
WOS记录号WOS:000365196100001
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55720
专题北京大学口腔医学院_口腔颌面外科
北京大学口腔医学院_牙周科
作者单位1.Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China
2.Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401147, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Lab Interdisciplinary Studies, Dept Oral & Maxillofacial Surg, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Gao, Xiang,Zhang, Xiaohong,Song, Jinlin,et al. Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2015,10:7109-7128.
APA Gao, Xiang.,Zhang, Xiaohong.,Song, Jinlin.,Xu, Xiao.,Xu, Anxiu.,...&Wei, Shicheng.(2015).Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering.INTERNATIONAL JOURNAL OF NANOMEDICINE,10,7109-7128.
MLA Gao, Xiang,et al."Osteoinductive peptide-functionalized nanofibers with highly ordered structure as biomimetic scaffolds for bone tissue engineering".INTERNATIONAL JOURNAL OF NANOMEDICINE 10(2015):7109-7128.
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