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学科主题: 临床医学
题名:
Characterization of anti-mesangial cell antibodies and their target antigens in patients with lupus nephritis
作者: Du, H; Chen, M; Zhang, Y; Zhao, MH
关键词: lupus nephritis ; anti-mesangial cell antibody ; human mesangial cell
刊名: JOURNAL OF CLINICAL IMMUNOLOGY
发表日期: 2005-05-01
DOI: 10.1007/s10875-005-4082-6
卷: 25, 期:3, 页:281-287
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: GLOMERULAR MESANGIAL CELLS ; ALPHA-ENOLASE ; DNA ANTIBODIES ; BINDING ANTIBODIES ; REVISED CRITERIA ; IGA NEPHROPATHY ; ERYTHEMATOSUS ; AUTOANTIBODIES ; CLASSIFICATION ; DISORDERS
英文摘要:

The pathogenetic mechanisms of lupus nephritis (LN) remain to be elucidated. In our previous study, autoantibodies against human glomerular mesangial cells (HMC) were identified in sera of most patients with lupus nephritis. The cur-rent study is to investigate the binding characteristics of anti-mesangial cell antibodies to human mesangial cell membrane. Serum samples were collected from 54 patients with renal biopsy proven lupus nephritis, 12 patients with systemic lupus erythematosus without clinical renal involvement, and 15 healthy Subjects. Membrane proteins were obtained from in vitro cultured HNIC by sonication and sequential centrifugation. DNase I were employed to remove DNA fragments in sera and membrane protein preperation and IgG F(ab′)(2) was obtained by pepsin digestion. Western Blot analysis was used to characterize the antibody and antigen interaction. In results, 25 of 54 (46.3%) sera from patients with lupus nephritis had anti-mesangial cell antibodies targeted at 74 kDa, 63 kDa, 52 kDa and 42 kDa protein bands of HNIC membrane. Only four of 12 (33.3%) sera from patients without renal involovement recognized the protein bands at 74 kDa and 63 kDa, but not 52 kDa and 42 kDa. DNase treatment of the HMC membrane and the sera did not affect the binding. IgG F(ab′)2 from sera of 10 patients with positive anti-mesangial cell antibodies could still bind the 63 kDa protein. In conclusion, anti-mesangial cell antibodies from sera of patients with lupus nephritis could bind membrane proteins of HMC directly without a DNA bridge and the binding was through antigen-anti body interation. Anti-mesangial cell antibodies might play some role in the pathogenesis of lupus nephritis(LN).

语种: 英语
WOS记录号: WOS:000230310600013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55732
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Inst Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Du, H,Chen, M,Zhang, Y,et al. Characterization of anti-mesangial cell antibodies and their target antigens in patients with lupus nephritis[J]. JOURNAL OF CLINICAL IMMUNOLOGY,2005,25(3):281-287.
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