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学科主题: 口腔医学
题名:
Magnetic bead-based salivary peptidome profiling analysis during orthodontic treatment durations
作者: Zhang, Jieni2; Zhou, Shaonan2; Zheng, Hui2; Zhou, Yanheng2; Chen, Feng1; Lin, Jiuxiang2
关键词: Orthodontic treatment duration ; Peptidome ; Saliva ; MALDI-TOF MS
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2012-05-18
DOI: 10.1016/j.bbrc.2012.04.100
卷: 421, 期:4, 页:844-849
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: BREAST-CANCER PATIENTS ; BIOMARKER DISCOVERY ; TOOTH MOVEMENT ; MASS-SPECTROMETRY ; PROTEOMICS ; OBESITY ; STAGE
英文摘要:

Orthodontic treatment induces various biological responses, including tooth movement and remodeling of alveolar bone. Although some studies have investigated the contribution of orthodontic procedures to changes in saliva conditions, little is known about the effects of different treatment durations on the saliva proteome. To identify the discriminating protein profiles in unstimulated whole saliva of orthodontic patients with different treatment durations, we used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic bead, and peptide mass fingerprints were created by scanning MS signals. Saliva samples from 40 patients (10 in each of four groups: the group without an appliance and groups under treatment for 2, 7, and 12 months) were analyzed. The results showed eight mass peaks with significant differences. Furthermore, mass peak intensities at proteins 1817.7, 2010.7, 2744 and 2710.2 Da represented a steady time-dependent increasing trend, whereas protein 4134 Da exhibited a decreasing tendency. Differential expression of the peptidome profile also occurred in the multiple comparisons, and we established a fitting model. Thus, the potential discriminating biomarkers investigated in this study reflected the complicated changes in periodontal tissues during orthodontic treatment and indicated dynamic interactions between orthodontic treatment and the saliva proteome. The results provide novel insights into alterations in salivary proteins due to different orthodontic treatment durations and may lead to the development of a therapeutic monitoring strategy for orthodontics. (C) 2012 Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: PKUSS20110301
项目资助者: Peking University School of Stomatology
WOS记录号: WOS:000305168600036
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55734
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Cent Lab, Beijing 100081, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Orthodont, Beijing 100081, Peoples R China

Recommended Citation:
Zhang, Jieni,Zhou, Shaonan,Zheng, Hui,et al. Magnetic bead-based salivary peptidome profiling analysis during orthodontic treatment durations[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2012,421(4):844-849.
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