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学科主题基础医学
Hypoxia-induced Up-regulation of Aquaporin-1 Protein in Prostate Cancer Cells in a p38-dependent Manner
Tie, Lu1,2; Lu, Ning1,2; Pan, Xue-Yang1,2; Pan, Yan1,2; An, Yu1,2; Gao, Jun-Wei1,2; Lin, Yan-Hua1,2; Yu, He-Ming3; Li, Xue-Jun1,2
关键词Aquaporin-1 (Aqp1) Hypoxia P38 Mapk Pkc Calcium Cancer Cells
刊名CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
2012
DOI10.1159/000337608
29期:1-2页:269-280
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Physiology
研究领域[WOS]Cell Biology ; Physiology
关键词[WOS]WATER CHANNELS ; INDUCIBLE FACTOR-1 ; KINASE-C ; PHOSPHATIDYLINOSITOL 3&prime ; -KINASE ; INDUCED PROLIFERATION ; SIGNALING PATHWAYS ; AQP1 EXPRESSION ; BRAIN-TUMORS ; GENE ; ACTIVATION
英文摘要

Background/Aims: Aquaporin-1 (AQP1) is a glycoprotein that mediates osmotic water transport, its expression has been found to correlate with tumour stage in some tumours. However, the mechanism by which AQP1 protein expression is regulated in tumor cells remains to be fully elucidated. We hypothesized that hypoxia might play an important role in AQP1 induction during tumorigenesis and at the late stages of tumor development. Methods: Isotonic and serum-free hypoxic models were used to investigate AQP1 expression in PC-3M human prostate cancer cells. Results: AQP1 expression was up-regulated by density-induced pericellular hypoxia and cobalt(II) chloride (CoCl 2)-induced hypoxia at the transcriptional level. Moreover, phosphorylation of p38 mitogen-activated protein kinase (MAPK) was induced by density-induced pericellular hypoxia and CoCl 2 induced hypoxia, specific inhibitors of p38 MAPK could concentration-dependently block those effects of hypoxia on AQP1 expression. Intracellular calcium ion (Ca2+) and protein kinase C (PKC) were shown to be responsible for the activation of p38 MAPK pathway. In addition, AQP1 induction in dense cultures was dependent on lowered oxygen (O-2) tension. In high cell density culture, certain secretory proteins might induce AQP1 expression indirectly. Conclusion: These findings suggest that AQP1 could be induced by hypoxia at transcription level, and the regulation of AQP1 in PC-3M cells is dependent on calcium, PKC and p38 MAPK, as well as low oxygen tension. Copyright (C) 2012 S. Karger AG, Basel

语种英语
WOS记录号WOS:000301141400028
项目编号30901803 ; 91129727 ; 81020108031 ; 30973558 ; 30572202 ; 30901815 ; 20090001120046 ; 2009ZX09103-144 ; B07001
资助机构National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Ministry of Science and Technology in China ; Ministry of Education of China
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55753
专题北京大学基础医学院_药理学系
北京大学医学部管理机构_医学部
北京大学基础医学院
作者单位1.Peking Univ, Inst Syst Biomed, Beijing 100191, Peoples R China
2.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
3.Natl Res Inst Family Planning, Beijing, Peoples R China
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GB/T 7714
Tie, Lu,Lu, Ning,Pan, Xue-Yang,et al. Hypoxia-induced Up-regulation of Aquaporin-1 Protein in Prostate Cancer Cells in a p38-dependent Manner[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2012,29(1-2):269-280.
APA Tie, Lu.,Lu, Ning.,Pan, Xue-Yang.,Pan, Yan.,An, Yu.,...&Li, Xue-Jun.(2012).Hypoxia-induced Up-regulation of Aquaporin-1 Protein in Prostate Cancer Cells in a p38-dependent Manner.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,29(1-2),269-280.
MLA Tie, Lu,et al."Hypoxia-induced Up-regulation of Aquaporin-1 Protein in Prostate Cancer Cells in a p38-dependent Manner".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 29.1-2(2012):269-280.
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