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学科主题: 临床医学
题名:
Inhibition of T-cell activition with HLA-DR1/DR4 restricted non-T-cell stimulating peptides
作者: Zhou, QA; Cheng, YJ; Lu, HS; Zhou, WH; Li, ZG
关键词: activation ; HLA-DR ; altered peptides ; collagen II ; T cell
刊名: HUMAN IMMUNOLOGY
发表日期: 2003-09-01
DOI: 10.1016/S0198-8859(03)00143-5
卷: 64, 期:9, 页:857-865
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: COLLAGEN-INDUCED ARTHRITIS ; MYELIN BASIC-PROTEIN ; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS ; HISTOCOMPATIBILITY COMPLEX-MOLECULES ; SHARED EPITOPE HYPOTHESIS ; ANTIGEN-PRESENTING CELLS ; HLA-DR MOLECULES ; MHC CLASS-II ; RHEUMATOID-ARTHRITIS ; SYNTHETIC COPOLYMER-1
英文摘要:

It has been reported that collagen II (CII) derived peptide CII263-272 induced T-cell activation via its amino acids responsible for T-cell receptor (TCR) recognition. The impact of substitution of the TCR contacting amino acids of CII263-272 on T-cell activation was evaluated in this study using a panel of altered CII263-272 peptides. Computer modeling revealed that the side chains of 263F and 266E in CII263-272 were coupled with amino acids on alpha1 and beta1 chains of HLA-DR1 or -DR4, mainly via hydrogen bonds, whereas the side chains of 267Q and 270K protrude out of the cleft and might be recognized by TCR. Intracellular delivery of the altered peptides, and their binding to HLA-DR1 and -DR4 molecules on cell surface, were demonstrated by confocal microscopy and flow cytometry. The results also revealed that the substitution of 267Q, 268G, 269P, and 270K individually or consecutively by alanine (A) or glycine (G) led to weak or non-T-cell responses. Furthermore, the altered peptides with 270K substitution (270A) or with consecutive substitution of 268G, 269P, and 270K (sub268-270) dramatically inhibited T-cell activation. It is suggested that the altered peptides derived from CII263-272 with substitution of amino acids responsible for TCR contact might be of inhibitory effect on T-cell responses. (C) American Society for Histocompatibility and Immunogenetics, 2003. Published by Elsevier Inc.

语种: 英语
WOS记录号: WOS:000185197900004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55764
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Med, Dept Rheumatol & Immunol, Peoples Hosp, Beijing 100871, Peoples R China
2.Peking Univ, Sch Med, Arthritis Res Inst, Peoples Hosp, Beijing 100871, Peoples R China
3.Tsing Hua Univ, Inst Biophys & Struct Biol, Beijing 100084, Peoples R China

Recommended Citation:
Zhou, QA,Cheng, YJ,Lu, HS,et al. Inhibition of T-cell activition with HLA-DR1/DR4 restricted non-T-cell stimulating peptides[J]. HUMAN IMMUNOLOGY,2003,64(9):857-865.
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