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学科主题基础医学
Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus
Zhao, JC; Wang, W; Wang, GF; Li, YH; Hui, Z; Xu, XY; Ren, F; Zhao, ZD; Gao, XM
关键词SARS-coronavirus spike glycoprotein ELISA antibodies
刊名JOURNAL OF CLINICAL VIROLOGY
2005-05-01
DOI10.1016/j.jcv.2004.09.024
33期:1页:12-18
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Virology
研究领域[WOS]Virology
关键词[WOS]ACUTE-RESPIRATORY-SYNDROME ; ANGIOTENSIN-CONVERTING ENZYME-2 ; FUNCTIONAL RECEPTOR ; STRUCTURAL PROTEINS ; GLYCOPROTEIN ; EXPRESSION ; VIRUS ; S1
英文摘要

Back-ground: Severe acute respiratory syndrome (SARS) is caused by infection with SARS-associated coronavirus (CoV). Amino acid residues 450-650 of the spike (S) glycoprotein of SARS-CoV (S450-650) contains dominant epitopes for anti-viral antibodies (Abs) in patient sera.

Objectives: To develop and evaluate an ELISA system for detection of anti-S Abs in patient sera. Study design: Express recombinant S450-650 in E. Coli and evaluate the sensitivity and specificity of an ELISA system based oil the S450-650 polypeptide.

Results: The S450-650-based ELISA detected IgG Abs in 41 out of 51 serum samples from 22 hospitalized patients with probable SARS, a result closely correlated with that obtained with a virus-based ELISA (r = 0.75, k = 0.8). Differential anti-S IgG responses were observed amongst SARS patients. Some of them produced anti-S Abs early during their infection, while others failed to make IgG Abs against the S450-650 polypeptide. None of the serum samples from 100 healthy blood donors was positive in the S450-650-based assay.

Conclusion: The S450-650-based ELISA can detect anti-S IgG Abs with high sensitivity and specificity. © 2004 Published by Elsevier B.V.

语种英语
WOS记录号WOS:000229030500003
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55774
Collection北京大学基础医学院_免疫学系
作者单位1.Beijing Red Cross Blood Ctr, Beijing 100088, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Affilated Hosp 1, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Zhao, JC,Wang, W,Wang, GF,et al. Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus[J]. JOURNAL OF CLINICAL VIROLOGY,2005,33(1):12-18.
APA Zhao, JC.,Wang, W.,Wang, GF.,Li, YH.,Hui, Z.,...&Gao, XM.(2005).Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus.JOURNAL OF CLINICAL VIROLOGY,33(1),12-18.
MLA Zhao, JC,et al."Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus".JOURNAL OF CLINICAL VIROLOGY 33.1(2005):12-18.
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