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学科主题: 基础医学
题名:
Enhanced MHC class I and costimulatory molecules on B16F10 cells by Ganoderma lucidum polysaccharides
作者: Sun, Li-Xin1,2; Lin, Zhi-Bin1; Duan, Xin-Suo2; Lu, Jie2; Ge, Zhi-Hua2; Li, Xue-Fei2; Li, Xue-Jun1; Li, Min1; Xing, En-Hong2; Song, You-Xin2; Jia, Jing2; Li, Wei-Dong1
关键词: Ganoderma lucidum polysaccharides ; tumor ; MHC class I ; costimulatory molecule
刊名: JOURNAL OF DRUG TARGETING
发表日期: 2012-08-01
DOI: 10.3109/1061186X.2012.697167
卷: 20, 期:7, 页:582-592
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: MAJOR HISTOCOMPATIBILITY COMPLEX ; DENDRITIC CELLS ; IMMUNE-RESPONSE ; MELANOMA-CELLS ; T-LYMPHOCYTES ; CANCER CELL ; MECHANISMS ; PROLIFERATION ; INHIBITION ; ACTIVATION
英文摘要:

Purpose: It is obvious that malignant cells evade from immune system in patients with manifest malignancy. Deficient major histocompatibility complex (MHC) class I and costimulatory molecules on malignant cells partially consist of evasion strategy since antigen bond MHC and costimulatory molecules provide two signals necessary for T cell activation. Therefore, enhancement of MHC-I and costimulatory molecules may favor restraint of the evasion. For this purpose, Ganoderma lucidum Polysaccharides (Gl-PS) was used on B16F10 melanoma cells in this study. Methods: Immunocytochemistry and flowcytometry were used to determine the H-2Kb and H-2Db (two prominent MHC class I molecules in C57BL mouse) as well as B7-1 and B7-2 (two prominent costimulatory molecules) expression on B16F10 cells after incubation with Gl-PS, while messenger ribonucleic acid (mRNA) of these molecules was detected by reverse transcription polymerase chain reaction (RT-PCR). Results: The H-2Kb and H-2Db, and B7-1 and B7-2 on B16F10 cells and mRNAs of these molecules were enhanced by Gl-PS, and more efficient antitumor cytotoxicity was induced by the Gl-PS treated cells. Conclusions: The MHC class I molecules and costimulatory molecules may be enhanced by Gl-PS, and more efficient immune cell mediated cytotoxicity against these B16F10 cells may be induced, which may favor cancer therapy.

语种: 英语
WOS记录号: WOS:000306001100004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55780
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Pharmacol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Chengde Med Coll, Affiliated Hosp, Chengde, Hebei Province, Peoples R China

Recommended Citation:
Sun, Li-Xin,Lin, Zhi-Bin,Duan, Xin-Suo,et al. Enhanced MHC class I and costimulatory molecules on B16F10 cells by Ganoderma lucidum polysaccharides[J]. JOURNAL OF DRUG TARGETING,2012,20(7):582-592.
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