IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
Developmental expression and function of DKKL1/Dkkl1 in humans and mice
Yan, Qiuxia2; Wu, Xiaoping4; Chen, Cairong2; Diao, Ruiying3; Lai, Yongqing3; Huang, Jun3; Chen, Jing3; Yu, Zhou3; Gui, Yaoting3; Tang, Aifa1; Cai, Zhiming1
关键词Dkkl1/dkkl1 Affymetrix Genechip Testis Spermatogenesis
刊名REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
2012-07-21
DOI10.1186/1477-7827-10-51
10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Endocrinology & Metabolism ; Reproductive Biology
资助者National Natural Science Foundation of China ; Shenzhen Important Foundation of Science Technology ; Shenzhen Basic Research Funds for Distinguished Young Scientists ; Qingyuan Foundation of Science Technology ; National Natural Science Foundation of China ; Shenzhen Important Foundation of Science Technology ; Shenzhen Basic Research Funds for Distinguished Young Scientists ; Qingyuan Foundation of Science Technology
研究领域[WOS]Endocrinology & Metabolism ; Reproductive Biology
关键词[WOS]TESTIS-SPECIFIC GENE ; DICKKOPF FAMILY ; SPERMATOGENESIS ; PROTEIN ; IDENTIFICATION ; SPERM ; SPERMIOGENESIS ; ROLES
英文摘要

Background: Experiments were designed to identify the developmental expression and function of the Dickkopf-Like1 (DKKL1/Dkkl1) gene in humans and mice.

Methods: Mouse testes cDNA samples were collected at multiple postnatal times (days 4, 9, 18, 35, and 54, as well as at 6 months) and hybridized to Affymetrix mouse whole genome Genechips. To further characterize the homologous gene DKKL1 in human beings, the expression profiles between human adult testis and foetal testis were compared using Affymetrix human Genechips. The characteristics of DKKL1/Dkkl1 were analysed using various cellular and molecular biotechnologies.

Results: The expression of Dkkl1 was not detected in mouse testes on days 4 or 9, but was present on days 18, 35, and 54, as well as at 6 months, which was confirmed by RT-PCR and Western blot results. Examination of the tissue distribution of Dkkl1 demonstrated that while Dkkl1 mRNA was abundantly expressed in testes, little to no expression of Dkkl1 was observed in the epididymis or other tissues. In an in vitro fertilization assay, a Dkkl1 antibody was found to significantly reduce fertilization. Human Genechips results showed that the hybridization signal intensity of DKKL1 was 405.56-fold higher in adult testis than in foetal testis. RT-PCR analysis of multiple human tissues indicated that DKKL1 mRNA was exclusively expressed in the testis. Western blot analysis also demonstrated that DKKL1 was mainly expressed in human testis with a molecular weight of approximately 34 kDa. Additionally, immunohistochemical staining showed that the DKKL1 protein was predominantly located in spermatocytes and round spermatids in human testes. An examination of the expression levels of DKKL1 in infertile male patients revealed that while no DKKL1 appeared in the testes of patients with Sertoli cell only syndrome (SCOS) or cryptorchidism, DKKL1 was observed with variable expression in patients with spermatogenic arrest.

Conclusions: These results, together with previous studies, suggest that DKKL1/Dkkl1 may play an important role in testicular development and spermatogenesis and may be an important factor in male infertility.

语种英语
所属项目编号30972992 ; 81170613 ; 81101922 ; 201001015 ; 2010B001 ; 2010B006
资助者National Natural Science Foundation of China ; Shenzhen Important Foundation of Science Technology ; Shenzhen Basic Research Funds for Distinguished Young Scientists ; Qingyuan Foundation of Science Technology ; National Natural Science Foundation of China ; Shenzhen Important Foundation of Science Technology ; Shenzhen Basic Research Funds for Distinguished Young Scientists ; Qingyuan Foundation of Science Technology
WOS记录号WOS:000308911000001
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55790
专题北京大学深圳医院
作者单位1.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen, Peoples R China
2.Jinan Univ, Dept Obstet & Gynecol, Affiliated Hosp 5, Peoples Hosp Qingyuan,Ctr Reprod Med,Coll Med, Qingyuan, Peoples R China
3.Peking Univ, Shenzhen Hosp, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen, Peoples R China
4.Jinan Univ, Inst Tissue Transplantat & Immunol, Guangzhou, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Yan, Qiuxia,Wu, Xiaoping,Chen, Cairong,et al. Developmental expression and function of DKKL1/Dkkl1 in humans and mice[J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,2012,10.
APA Yan, Qiuxia.,Wu, Xiaoping.,Chen, Cairong.,Diao, Ruiying.,Lai, Yongqing.,...&Cai, Zhiming.(2012).Developmental expression and function of DKKL1/Dkkl1 in humans and mice.REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY,10.
MLA Yan, Qiuxia,et al."Developmental expression and function of DKKL1/Dkkl1 in humans and mice".REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 10(2012).
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