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学科主题: 药学
题名:
Loss of silencing activity caused by 5 ′-terminal modification with D/L-isonucleotide (isoNA) or locked nucleic acid (LNA) could not be restored by 5 ′-terminal phosphorylation
作者: Huang Ye; Chen Zhuo; Wang Zhuo; Li YaTing; Chen Yue; Yang ZhenJun1; Zhang LiHe
关键词: isonucleotide ; locked nucleic acid (LNA) ; 5 &prime ; -terminal phosphorylation
刊名: SCIENCE CHINA-CHEMISTRY
发表日期: 2014-02-01
DOI: 10.1007/s11426-013-5032-4
卷: 57, 期:2, 页:329-334
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: CHEMICAL-MODIFICATION ; MODIFIED SIRNAS ; IN-VITRO ; RNA ; STABILITY ; OLIGONUCLEOTIDES ; INTERFERENCE ; COMPLEX ; VIVO
英文摘要:

In this study, a series of 5′-phosphorylated siRNAs with D/L-isonucleotide (isoNA) or locked nucleic acid (LNA) incorporated at the 5′-terminus were synthesized. It was found that after incorporating either isoNA or LNA at the 5′-terminus of the antisense strand or sense strand, the silencing activity of modified strands has been inhibited, which cannot be recovered by phosphorylation at the 5′-terminus; however, the silencing activity of unmodified strand to its own target was increased. This work indicates that the isoNA and LNA modification at 5′-terminus can interfere with the strand selection during the RISC assembling process, and the disturbance of the 5′-phosporylation should not be the only viable mechanism.

语种: 英语
所属项目编号: 20932001 ; 2009ZX09503 ; 2012AA022501 ; 2012CB720604
项目资助者: National Natural Sciences Foundation of China ; Program of the Ministry of Science and Technology of China
WOS记录号: WOS:000330681700016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55791
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Huang Ye,Chen Zhuo,Wang Zhuo,et al. Loss of silencing activity caused by 5 ′-terminal modification with D/L-isonucleotide (isoNA) or locked nucleic acid (LNA) could not be restored by 5 ′-terminal phosphorylation[J]. SCIENCE CHINA-CHEMISTRY,2014,57(2):329-334.
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