北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
Use of PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection
作者: Zhang, ZH; Ohkohchi, N; Okazaki, H; Guo, YL
关键词: acute rejection ; PCR ; renal transplantation ; urine ; DNA
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2003-02-01
卷: 116, 期:2, 页:191-194
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: PERIPHERAL-BLOOD CHIMERISM ; LUNG TRANSPLANTATION ; CELL-MIGRATION ; AMPLIFICATION ; LYMPHOCYTES ; PRIMERS
英文摘要:

Objective To analyze the urine of renal recipients for the presence of donor DNA in an attempt to establish an alternative diagnostic means of acute rejection.

Methods Sixty-four renal transplant recipients were examined. Thirty-seven were normal after transplantation, while 22 others developed acute rejection, based on serum creatinine levels and/or needle biopsy findings of the graft. Five developed drug-induced renal dysfunction. In female recipients with a male graft, we examined urine for the presence of Y chromosome (SRY and DYZ(-1)) and in recipients receiving an HLA mismatched graft, we looked for HLA-DR gene (DRB1) using PCR.

Results Among the 14 female recipients with male grafts demonstrating stable renal function, only one was positive for SRY and DYZ-1 on the Y chromosome. However, SRY and DYZ-1 were found in the urine of four female patients with acute rejection, but these DNA fragments were not detected in 3 of the 4 after anti-rejection therapy. The last patient was referred to hemodialysis. Of 23 recipients of a graft from HLA mismatch donors with stable renal function, DRB1 was negative in 21 (91%). Of 18 patients with acute rejection, DRB1 was positive in 16 (89%) and negative in 2. These DNA fragments were no longer found in 13 patients after anti-rejection therapy. In all patients with drug induced renal dysfunction, donor-derived DNA was negative.

Conclusions Presence of door specific DNA in the urine of the recipient is strongly associated with acute rejection. Analysis of DNA derived from donor cells in urine was an effective and accurate method for the diagnosis of acute rejection of a renal transplant.

语种: 英语
WOS记录号: WOS:000181816500007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55817
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Sendai Shakaihoken Hosp, Dept Surg, Sendai, Miyagi 9810912, Japan
2.Peking Univ, Inst Urol, Mol Biol Unit, Hosp 1, Beijing 100034, Peoples R China
3.Tohoku Univ, Grad Sch Med, Div Adv Surg Sci & Technol, Sendai, Miyagi 9808574, Japan

Recommended Citation:
Zhang, ZH,Ohkohchi, N,Okazaki, H,et al. Use of PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection[J]. CHINESE MEDICAL JOURNAL,2003,116(2):191-194.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, ZH]'s Articles
[Ohkohchi, N]'s Articles
[Okazaki, H]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, ZH]‘s Articles
[Ohkohchi, N]‘s Articles
[Okazaki, H]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace