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Use of PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection
Zhang, ZH; Ohkohchi, N; Okazaki, H; Guo, YL
关键词Acute Rejection Pcr Renal Transplantation Urine Dna
刊名CHINESE MEDICAL JOURNAL
2003-02-01
116期:2页:191-194
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]PERIPHERAL-BLOOD CHIMERISM ; LUNG TRANSPLANTATION ; CELL-MIGRATION ; AMPLIFICATION ; LYMPHOCYTES ; PRIMERS
英文摘要

Objective To analyze the urine of renal recipients for the presence of donor DNA in an attempt to establish an alternative diagnostic means of acute rejection.

Methods Sixty-four renal transplant recipients were examined. Thirty-seven were normal after transplantation, while 22 others developed acute rejection, based on serum creatinine levels and/or needle biopsy findings of the graft. Five developed drug-induced renal dysfunction. In female recipients with a male graft, we examined urine for the presence of Y chromosome (SRY and DYZ(-1)) and in recipients receiving an HLA mismatched graft, we looked for HLA-DR gene (DRB1) using PCR.

Results Among the 14 female recipients with male grafts demonstrating stable renal function, only one was positive for SRY and DYZ-1 on the Y chromosome. However, SRY and DYZ-1 were found in the urine of four female patients with acute rejection, but these DNA fragments were not detected in 3 of the 4 after anti-rejection therapy. The last patient was referred to hemodialysis. Of 23 recipients of a graft from HLA mismatch donors with stable renal function, DRB1 was negative in 21 (91%). Of 18 patients with acute rejection, DRB1 was positive in 16 (89%) and negative in 2. These DNA fragments were no longer found in 13 patients after anti-rejection therapy. In all patients with drug induced renal dysfunction, donor-derived DNA was negative.

Conclusions Presence of door specific DNA in the urine of the recipient is strongly associated with acute rejection. Analysis of DNA derived from donor cells in urine was an effective and accurate method for the diagnosis of acute rejection of a renal transplant.

语种英语
WOS记录号WOS:000181816500007
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55817
专题北京大学第一临床医学院_泌尿外科
作者单位1.Sendai Shakaihoken Hosp, Dept Surg, Sendai, Miyagi 9810912, Japan
2.Peking Univ, Inst Urol, Mol Biol Unit, Hosp 1, Beijing 100034, Peoples R China
3.Tohoku Univ, Grad Sch Med, Div Adv Surg Sci & Technol, Sendai, Miyagi 9808574, Japan
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GB/T 7714
Zhang, ZH,Ohkohchi, N,Okazaki, H,et al. Use of PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection[J]. CHINESE MEDICAL JOURNAL,2003,116(2):191-194.
APA Zhang, ZH,Ohkohchi, N,Okazaki, H,&Guo, YL.(2003).Use of PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection.CHINESE MEDICAL JOURNAL,116(2),191-194.
MLA Zhang, ZH,et al."Use of PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection".CHINESE MEDICAL JOURNAL 116.2(2003):191-194.
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