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学科主题: 临床医学
题名:
Electron paramagnetic resonance in monitoring of nitric oxide production after kidney transplantation in rats
作者: Xu, T; Chen, X; Wang, XF; Huang, XB; Qu, XK; Ye, HY; Zhang, XD; Hou, SK; Zhu, JC
关键词: nitric oxide ; kidney transplantation ; ischemia/reperfusion injury ; electron paramagnetic resonance
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2004-10-01
卷: 117, 期:10, 页:1552-1557
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: ISCHEMIA-REPERFUSION INJURY ; RENAL ISCHEMIA/REPERFUSION ; FREE-RADICALS ; L-ARGININE ; ENDOTHELIUM ; FAILURE ; PREVENTION ; MECHANISM ; ADHESION ; TISSUES
英文摘要:

Background Much research has been focused on ischemia/reperfusion injury ( IRI) to the transplanted organs. As a free radical, nitric oxide (NO) plays an important role in IRI. In this study, the production of NO and its functions during IRI were monitored in rat models after allotransplantation of kidney grafts.

Methods Of 75 male LEW rats, 30 served as donors, and the remaining 45 rats were divided into three groups ( 15 rats in each group) : controls ( group 1), kidney allotransplantation followed by bilateral nephrectomy during reperfusion (group 2), 2 hours before operation, donors and recipients were treated with N-G-nitro L-arginine methyl ester ( L-NAME), a NO synthase inhibitor, at a dose of 30 mg/kg (group 3). Bilateral nephrectomies were performed while kidney grafts were reperfused. The kidney grafts were hypothemically stored for 24 hours. The production of NO before and after reperfusion was measured by electron paramagnetic resonance ( EPR). The creatinine level, the glomerular filtration rate (GFR) and the protein carbonyl content in tissue samples were recorded on the first and the fifth day after operation. The data were evaluated by one-way analysis of variance. Differences were considered to be statistically significant when a P value was less than 0.05.

Results After reperfusion for 15 minutes, the production of NO increased remarkably and kept increasing till 120 minutes, after which the level returned to normal. In group 3, which was pretreated with L-NAME, creatinine levels were higher than those in group 2 at the 24th hour (4.10 +/- 0.50 mg/dl vs. 3.77 +/- 0.42 mg/dl, P < 0.05) and the 120th hour (3.19 +/- 0.79 mg/dl vs. 2.22 +/- 0.53 mg/dl, P < 0.05). GFR levels in group 3 were lower than those in group 2 at the 24th hour (0.50 +/- 0.12 ml/min vs. 0.71 +/- 0.19 ml/min, P < 0.05) and the 120th hour (0.59 +/- 0.38 ml/min vs. 1.27 +/- 0.23 ml/min, P < 0.01). The content of protein carbonyl in tissue samples of group 3 was lower than that in group 2 at the 24th hour (29.01 +/- 7.02 nmol/mg protein vs. 49.39 +/- 13.13 nmol/mg protein, P < 0.05), but was higher than that at the 120th hour (75.71 +/- 16.74 nmol/mg protein vs. 57.93 +/- 15.32 nmol/mg protein, P < 0.05).

Conclusions After transplantation of hypothemically stored kidney grafts, the increased NO production in the early stage has protective effects on the transplanted kidney. Application of L-NAME to inhibit NO production is harmful to the recovery of the renal functions of kidney grafts.

语种: 英语
WOS记录号: WOS:000224787500021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55819
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Urol, Beijing 100044, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Cell Biol & Genet, Beijing 100083, Peoples R China

Recommended Citation:
Xu, T,Chen, X,Wang, XF,et al. Electron paramagnetic resonance in monitoring of nitric oxide production after kidney transplantation in rats[J]. CHINESE MEDICAL JOURNAL,2004,117(10):1552-1557.
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