IR@PKUHSC  > 北京大学基础医学院  > 细胞生物学系
学科主题基础医学
cAMP Responsive Element Binding Protein-1 Is a Transcription Factor of Lysosomal-Associated Protein Transmembrane-4 beta in Human Breast Cancer Cells
Zhang, Meng1; Xu, Jian-Jun1; Zhou, Rou-Li2; Zhang, Qing-Yun1
刊名PLOS ONE
2013-02-28
DOI10.1371/journal.pone.0057520
8期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; GALLBLADDER CARCINOMA ; DOWN-REGULATION ; CREB ; LAPTM4B-35 ; EXPRESSION ; OVEREXPRESSION ; GENE ; PHOSPHORYLATION ; RESISTANCE
英文摘要

Lysosomal-associated protein transmembrane-4 beta (LAPTM4B) is a potential proto-oncogene, whose overexpression is involved in cancer occurrence and progression. Its transcript is up-regulated in various types of solid tumors including breast cancer. However, its transcriptional regulation mechanism is still unclear. To investigate the mechanism of transcriptional regulation of LAPTM4B in human breast cancer cells, a series of luciferase reporter constructs and construct with mutated binding site for cAMP responsive element binding protein-1 (CREB1) were generated by PCR amplification and transiently transfected into breast cancer cells to determine the transcriptional activities of different promoter regions. The + 10 similar to+ 292 promoter region was possessed the highest transcriptional activity. The ability of CREB1 to bind the LAPMT4B promoter was confirmed by electrophoretic mobility shift assay, super-shift and RNA interference experiments. Our study identified the core promoter region responsible for constitutive expression of LAPTM4B and clarified that CREB1 played an important role in LAPTM4B transcriptional regulation in human breast cancer cells.

语种英语
所属项目编号81071422
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000315524900112
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55826
Collection北京大学基础医学院_细胞生物学系
作者单位1.Peking Univ, Canc Hosp & Inst, Key Lab Carcinogenesis & Translat Res, Minist Educ,Dept Clin Lab, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Cell Biol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Meng,Xu, Jian-Jun,Zhou, Rou-Li,et al. cAMP Responsive Element Binding Protein-1 Is a Transcription Factor of Lysosomal-Associated Protein Transmembrane-4 beta in Human Breast Cancer Cells[J]. PLOS ONE,2013,8(2).
APA Zhang, Meng,Xu, Jian-Jun,Zhou, Rou-Li,&Zhang, Qing-Yun.(2013).cAMP Responsive Element Binding Protein-1 Is a Transcription Factor of Lysosomal-Associated Protein Transmembrane-4 beta in Human Breast Cancer Cells.PLOS ONE,8(2).
MLA Zhang, Meng,et al."cAMP Responsive Element Binding Protein-1 Is a Transcription Factor of Lysosomal-Associated Protein Transmembrane-4 beta in Human Breast Cancer Cells".PLOS ONE 8.2(2013).
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