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学科主题口腔医学
Enhanced Biological Behavior of In Vitro Human Gingival Fibroblasts on Cold Plasma-Treated Zirconia
Zheng, Miao1; Yang, Yang1; Liu, Xiao-Qiang1; Liu, Ming-Yue1; Zhang, Xiao-Fei2; Wang, Xin3; Li, He-Ping2; Tan, Jian-Guo1
刊名PLOS ONE
2015-10-13
DOI10.1371/journal.pone.0140278
10期:10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; Foundation of Peking University School of Stomatology ; Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Foundation of Peking University School of Stomatology ; Research Fund for the Doctoral Program of Higher Education
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]SOFT-TISSUE REACTIONS ; INITIAL ATTACHMENT ; SURFACE-TOPOGRAPHY ; TITANIUM SURFACES ; DENTAL IMPLANT ; FLEXURAL STRENGTH ; FUNCTIONAL-GROUPS ; GENE-EXPRESSION ; CELL-ADHESION ; WETTABILITY
英文摘要

Objective

To evaluate whether atmospheric-pressure dielectric-barrier-discharge plasma treatment of zirconia enhances its biocompatibility with human gingival fibroblasts.

Materials and Methods

The zirconia disks were divided into four groups and treated using helium atmospheric-pressure dielectric-barrier-discharge plasmas for 30, 60 or 90 s or left untreated. The surface morphology, wettability and chemical elements were analyzed. Fibroblasts density, morphology, morphometry and attachment-related genes expression were measured at different time points from 3 to 72 h.

Results

After plasma treatment, the surface morphology and roughness remained the same, while the contact angle decreased from 78.31 degrees to 43.71 degrees, and the surface C/O ratio decreased from 3.17 to 0.89. The surficial areas and perimeters of HGFs were increased two-fold in the treated groups at 3 h. Fibroblasts density increased on treated disks at all time points, especially the ones treated for 60 s. Attachment-related genes in the groups treated for 30 and 60 s were significantly higher at 3 and 24 h.

Conclusion

The helium atmospheric-pressure dielectric-barrier-discharge plasma treatment enhances the biological behavior of fibroblasts on zirconia by increasing the expression of attachment-related genes within 24 h and promoting the cell density during longer culture times. Wettability of zirconia, an important physicochemical property, has a vital influence on the cell behaviors.

语种英语
所属项目编号81200807 ; 11475103 ; YS020212 ; 20120001110044
资助者National Natural Science Foundation of China ; Foundation of Peking University School of Stomatology ; Research Fund for the Doctoral Program of Higher Education ; National Natural Science Foundation of China ; Foundation of Peking University School of Stomatology ; Research Fund for the Doctoral Program of Higher Education
WOS记录号WOS:000362962300077
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55828
Collection北京大学口腔医学院_口腔修复科
作者单位1.Tsinghua Univ, Dept Engn Phys, Beijing 100084, Peoples R China
2.Peking Univ, Dept Prosthodont, Sch Stomatol, Beijing 100081, Peoples R China
3.North China Univ Sci & Technol, Coll Mech Engn, Tangshan 063009, Peoples R China
Recommended Citation
GB/T 7714
Zheng, Miao,Yang, Yang,Liu, Xiao-Qiang,et al. Enhanced Biological Behavior of In Vitro Human Gingival Fibroblasts on Cold Plasma-Treated Zirconia[J]. PLOS ONE,2015,10(10).
APA Zheng, Miao.,Yang, Yang.,Liu, Xiao-Qiang.,Liu, Ming-Yue.,Zhang, Xiao-Fei.,...&Tan, Jian-Guo.(2015).Enhanced Biological Behavior of In Vitro Human Gingival Fibroblasts on Cold Plasma-Treated Zirconia.PLOS ONE,10(10).
MLA Zheng, Miao,et al."Enhanced Biological Behavior of In Vitro Human Gingival Fibroblasts on Cold Plasma-Treated Zirconia".PLOS ONE 10.10(2015).
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