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学科主题: 基础医学
题名:
Caffeic Acid Inhibits Acute Hyperhomocysteinemia-Induced Leukocyte Rolling and Adhesion in Mouse Cerebral Venules
作者: Zhao, Hai-Ping1,3; Feng, Juan1; Sun, Kai3; Liu, Yu-Ying3; Wei, Xiao-Hong3; Fan, Jing-Yu3; Huang, Ping3; Mao, Xiao-Wei3; Zhou, Zhou1; Wang, Chuan-She2; Wang, Xian1; Han, Jing-Yan2,3
关键词: leukocyte adhesion molecule ; chemokine ; Src ; PI3K ; Akt
刊名: MICROCIRCULATION
发表日期: 2012-04-01
DOI: 10.1111/j.1549-8719.2011.00151.x
卷: 19, 期:3, 页:233-244
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: MONOCYTE CHEMOATTRACTANT PROTEIN-1 ; ENDOTHELIAL-CELLS ; THREONINE PHOSPHORYLATION ; INFLAMMATORY MARKERS ; LYMPHOCYTE FUNCTION ; CYTOPLASMIC DOMAIN ; HUMAN NEUTROPHILS ; OXIDATIVE STRESS ; KINASE-C ; HOMOCYSTEINE
英文摘要:

Objective: To investigate the effects and possible mechanisms of CA on acute HHcy-induced leukocyte rolling and adhesion in mouse cerebral venules. Methods: Male C57 BL/6J mice were injected with DL-Hcy (50 mg/kg) and CA (10 mg/kg). The effect of CA on HHcy-induced leukocyte rolling and adhesion in cerebral vessels was assessed using intravital microscopy. Plasma cytokines and chemokines were evaluated by cytometric bead array. ROS production in HUVECs and adhesion molecule expression on leukocytes were determined by flow cytometry. E-selectin and ICAM-1 expression in cerebrovascular endothelium was detected by immunohistochemistry. CD18 phosphorylation and the Src/PI3K/Akt pathway in leukocytes were determined by confocal microscopy and Western blot. Results: CA inhibited HHcy-elicited leukocyte rolling and adhesion, decreased ROS production in HUVECs, and reduced plasma KC, MIP-2, and MCP-1 levels. CA reduced the E-selectin and ICAM-1 expression on cerebrovascular endothelium and CD11b/CD18 on leukocytes caused by HHcy. Of notice, CA depressed CD18 phosphorylation and the Src/PI3K/Akt pathway in leukocytes. Conclusions: CA inhibited HHcy-provoked leukocyte rolling and adhesion in cerebral venules, ameliorating adhesion molecule expression and activation, which is related to the suppression of the Src/PI3K/Akt pathway in leukocytes.

语种: 英语
所属项目编号: 2008ZX09401 ; 30730042 ; 30821001 ; 2011CB503904
项目资助者: Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Basic Research Program of China
WOS记录号: WOS:000302074600006
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55835
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Peking Univ, Dept Physiol & Pathophysiol, Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Dept Integrat Tradit Chinese & Western Med, Sch Basic Med Sci, Beijing 100191, Peoples R China
3.Peking Univ, Tasly Microcirculat Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zhao, Hai-Ping,Feng, Juan,Sun, Kai,et al. Caffeic Acid Inhibits Acute Hyperhomocysteinemia-Induced Leukocyte Rolling and Adhesion in Mouse Cerebral Venules[J]. MICROCIRCULATION,2012,19(3):233-244.
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