北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 心血管所  > 期刊论文
学科主题: 基础医学
题名:
Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway
作者: Wang, Cheng1; Wen, Jianyan2; Zhou, Yun1; Li, Li1; Cui, Xiaobing3; Wang, Jinyu1; Pan, Lin4; Ye, Zhidong2; Liu, Peng2; Wu, Liling1
关键词: Atherosclerosis ; Vascular smooth muscle cells ; Apelin ; FoxO3a ; MMP-2
刊名: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
发表日期: 2015-12-01
DOI: 10.1016/j.biocel.2015.10.015
卷: 69, 页:173-182
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: HUMAN APJ RECEPTOR ; IN-VIVO ; PROLIFERATION ; ATHEROSCLEROSIS ; INHIBITION ; HEART ; METALLOPROTEINASES ; PHOSPHORYLATION ; MECHANISMS ; EXPRESSION
英文摘要:

Apelin is an adipokine that has a critical role in the development of atherosclerosis, which may offer potential for therapy. Because migration of vascular smooth muscle cells (VSMCs) is a key event in the development of atherosclerosis, understanding its effect on the atherosclerotic vasculature is needed. Here we investigated the effect of apelin on VSMC migration and the possible signaling mechanism. In cultured rat VSMCs, apelin dose- and time-dependently promoted VSMC migration. Apelin increased the phosphorylation of Akt, whereas LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3K), and an Akt1/2 kinase inhibitor blocked the apelin-induced VSMC migration. Apelin dose-dependently induced phosphorylation of Forkhead box O3a (FoxO3a) and promoted its translocation from the nucleus to cytoplasm, which were blocked by LY294002 and Akt1/2 kinase inhibitor. Furthermore, apelin increased matrix metalloproteinase 2 (MMP-2) expression and gelatinolytic activity. Overexpression of a constitutively active, phosphorylation-resistant mutant, TM-FoxO3a, in VSMCs abrogated the effect of apelin on MMP-2 expression and VSMC migration. ARP101, an inhibitor of MMP-2, suppressed apelin-induced VSMC migration. Moreover, the levels of apelin, phosphorylated Akt, FoxO3a, and MMP-2 were higher in human carotid-artery atherosclerotic plaque than in adjacent normal vessels. We demonstrate that PI3K/Akt/FoxO3a signaling may be involved in apelin inducing VSMC migration. Phosphorylation of FoxO3a plays a central role in mediating the apelin-induced MMP-2 activation and VSMC migration. (C) 2015 Published by Elsevier Ltd.

语种: 英语
所属项目编号: 30900565 ; 81070230 ; 2015-QNYC-B-07 ; 7112084 ; 2007CB512004 ; 2013DFA31900
项目资助者: National Natural Science Foundation of China ; China-Japan Friendship Hospital Youth Science and Technology Excellence Project ; Beijing Natural Science Foundation ; National Basic Research Program of China (973 Program) ; International S&amp ; T cooperation program
WOS记录号: WOS:000367020800019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55851
Appears in Collections:基础医学院_心血管所_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1-s2.0-S1357272515300406-main.pdf(4424KB)期刊论文作者接受稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci, Hlth Sci Ctr,Minist Educ, Beijing 100191, Peoples R China
2.China Japan Friendship Hosp, Dept Cardiovasc Surg, Beijing 100029, Peoples R China
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
4.China Japan Friendship Hosp, Inst Clin Med Sci, Beijing 100029, Peoples R China

Recommended Citation:
Wang, Cheng,Wen, Jianyan,Zhou, Yun,et al. Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2015,69:173-182.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Cheng]'s Articles
[Wen, Jianyan]'s Articles
[Zhou, Yun]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Cheng]‘s Articles
[Wen, Jianyan]‘s Articles
[Zhou, Yun]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace