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学科主题基础医学
Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway
Wang, Cheng1; Wen, Jianyan2; Zhou, Yun1; Li, Li1; Cui, Xiaobing3; Wang, Jinyu1; Pan, Lin4; Ye, Zhidong2; Liu, Peng2; Wu, Liling1
关键词Atherosclerosis Vascular Smooth Muscle Cells Apelin Foxo3a Mmp-2
刊名INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
2015-12-01
DOI10.1016/j.biocel.2015.10.015
69页:173-182
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Natural Science Foundation of China ; China-Japan Friendship Hospital Youth Science and Technology Excellence Project ; Beijing Natural Science Foundation ; National Basic Research Program of China (973 Program) ; International S&amp ; T cooperation program ; National Natural Science Foundation of China ; China-Japan Friendship Hospital Youth Science and Technology Excellence Project ; Beijing Natural Science Foundation ; National Basic Research Program of China (973 Program) ; International S&amp ; T cooperation program
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]HUMAN APJ RECEPTOR ; IN-VIVO ; PROLIFERATION ; ATHEROSCLEROSIS ; INHIBITION ; HEART ; METALLOPROTEINASES ; PHOSPHORYLATION ; MECHANISMS ; EXPRESSION
英文摘要

Apelin is an adipokine that has a critical role in the development of atherosclerosis, which may offer potential for therapy. Because migration of vascular smooth muscle cells (VSMCs) is a key event in the development of atherosclerosis, understanding its effect on the atherosclerotic vasculature is needed. Here we investigated the effect of apelin on VSMC migration and the possible signaling mechanism. In cultured rat VSMCs, apelin dose- and time-dependently promoted VSMC migration. Apelin increased the phosphorylation of Akt, whereas LY294002, an inhibitor of phosphatidylinositol 3-kinase (PI3K), and an Akt1/2 kinase inhibitor blocked the apelin-induced VSMC migration. Apelin dose-dependently induced phosphorylation of Forkhead box O3a (FoxO3a) and promoted its translocation from the nucleus to cytoplasm, which were blocked by LY294002 and Akt1/2 kinase inhibitor. Furthermore, apelin increased matrix metalloproteinase 2 (MMP-2) expression and gelatinolytic activity. Overexpression of a constitutively active, phosphorylation-resistant mutant, TM-FoxO3a, in VSMCs abrogated the effect of apelin on MMP-2 expression and VSMC migration. ARP101, an inhibitor of MMP-2, suppressed apelin-induced VSMC migration. Moreover, the levels of apelin, phosphorylated Akt, FoxO3a, and MMP-2 were higher in human carotid-artery atherosclerotic plaque than in adjacent normal vessels. We demonstrate that PI3K/Akt/FoxO3a signaling may be involved in apelin inducing VSMC migration. Phosphorylation of FoxO3a plays a central role in mediating the apelin-induced MMP-2 activation and VSMC migration. (C) 2015 Published by Elsevier Ltd.

语种英语
所属项目编号30900565 ; 81070230 ; 2015-QNYC-B-07 ; 7112084 ; 2007CB512004 ; 2013DFA31900
资助者National Natural Science Foundation of China ; China-Japan Friendship Hospital Youth Science and Technology Excellence Project ; Beijing Natural Science Foundation ; National Basic Research Program of China (973 Program) ; International S&amp ; T cooperation program ; National Natural Science Foundation of China ; China-Japan Friendship Hospital Youth Science and Technology Excellence Project ; Beijing Natural Science Foundation ; National Basic Research Program of China (973 Program) ; International S&amp ; T cooperation program
WOS记录号WOS:000367020800019
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55851
专题基础医学院_心血管所
作者单位1.Peking Univ, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci, Hlth Sci Ctr,Minist Educ, Beijing 100191, Peoples R China
2.China Japan Friendship Hosp, Dept Cardiovasc Surg, Beijing 100029, Peoples R China
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptides, Beijing 100191, Peoples R China
4.China Japan Friendship Hosp, Inst Clin Med Sci, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714
Wang, Cheng,Wen, Jianyan,Zhou, Yun,et al. Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2015,69:173-182.
APA Wang, Cheng.,Wen, Jianyan.,Zhou, Yun.,Li, Li.,Cui, Xiaobing.,...&Wu, Liling.(2015).Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,69,173-182.
MLA Wang, Cheng,et al."Apelin induces vascular smooth muscle cells migration via a PI3K/Akt/FoxO3a/MMP-2 pathway".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 69(2015):173-182.
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