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Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing
Wang, Shuai1,2; Guo, Lihong2,4; Seneviratne, Chaminda Jayampath1; Huang, Bo2; Han, Jianmin3; Peng, Lei2; Liu, Xiaodi2; Zhang, Chengfei1
关键词Dental Restorative Materials Biofilm Denaturing Gradient Gel Electrophoresis Microbial Diversity
刊名DENTAL MATERIALS JOURNAL
2014-06-01
DOI10.4012/dmj.2013-152
33期:3页:325-331
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine ; Materials Science, Biomaterials
研究领域[WOS]Dentistry, Oral Surgery & Medicine ; Materials Science
关键词[WOS]GLASS-IONOMER CEMENTS ; STREPTOCOCCUS-MUTANS ; COMMUNITIES ; IDENTIFICATION ; FLUORIDE ; PLAQUE ; POPULATIONS ; COMPOSITE ; AMALGAM ; GENES
英文摘要

The microbial diversity of biofilms formed on the surfaces of amalgam, glass-ionomer cement, and resin composite was analyzed by denaturing gradient gel electrophoresis (DGGE). The V2-V3 region of salivary microbial 16S rDNA gene sequences of planktonic and biofilm bacteria, after 1 day and 1 week of incubation, was amplified by polymerase chain reaction (PCR) and analyzed by DGGE. The amounts of strongly adherent phylotypes after 1 day and 1 week on the three dental restorative materials were more than those on hydroxyapatite. Streptococcus salivarius was detected in both loosely adherent and strong adherent groups of all 1-day samples. At 1 week, the amounts of loosely adherent and strongly adherent phylotypes present on the three restorative materials ranked in this ascending order: glass-ionomer cement < resin composite < amalgam. Results of DGGE analysis suggested that glass-ionomer cement was the best material of choice in terms of suppressing bacterial phylotypes in biofilms.

语种英语
WOS记录号WOS:000340230900005
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55854
专题北京大学口腔医学院_口腔生物研究室
作者单位1.Univ Hong Kong, Fac Dent, Sai Ying Pun, Hong Kong, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Oral Biol, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dent Mat Lab, Beijing 100081, Peoples R China
4.Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA
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Wang, Shuai,Guo, Lihong,Seneviratne, Chaminda Jayampath,et al. Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing[J]. DENTAL MATERIALS JOURNAL,2014,33(3):325-331.
APA Wang, Shuai.,Guo, Lihong.,Seneviratne, Chaminda Jayampath.,Huang, Bo.,Han, Jianmin.,...&Zhang, Chengfei.(2014).Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing.DENTAL MATERIALS JOURNAL,33(3),325-331.
MLA Wang, Shuai,et al."Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing".DENTAL MATERIALS JOURNAL 33.3(2014):325-331.
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