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学科主题: 口腔医学
题名:
Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing
作者: Wang, Shuai1,2; Guo, Lihong2,4; Seneviratne, Chaminda Jayampath1; Huang, Bo2; Han, Jianmin3; Peng, Lei2; Liu, Xiaodi2; Zhang, Chengfei1
关键词: Dental restorative materials ; Biofilm ; Denaturing gradient gel electrophoresis ; Microbial diversity
刊名: DENTAL MATERIALS JOURNAL
发表日期: 2014-06-01
DOI: 10.4012/dmj.2013-152
卷: 33, 期:3, 页:325-331
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine ; Materials Science, Biomaterials
研究领域[WOS]: Dentistry, Oral Surgery & Medicine ; Materials Science
关键词[WOS]: GLASS-IONOMER CEMENTS ; STREPTOCOCCUS-MUTANS ; COMMUNITIES ; IDENTIFICATION ; FLUORIDE ; PLAQUE ; POPULATIONS ; COMPOSITE ; AMALGAM ; GENES
英文摘要:

The microbial diversity of biofilms formed on the surfaces of amalgam, glass-ionomer cement, and resin composite was analyzed by denaturing gradient gel electrophoresis (DGGE). The V2-V3 region of salivary microbial 16S rDNA gene sequences of planktonic and biofilm bacteria, after 1 day and 1 week of incubation, was amplified by polymerase chain reaction (PCR) and analyzed by DGGE. The amounts of strongly adherent phylotypes after 1 day and 1 week on the three dental restorative materials were more than those on hydroxyapatite. Streptococcus salivarius was detected in both loosely adherent and strong adherent groups of all 1-day samples. At 1 week, the amounts of loosely adherent and strongly adherent phylotypes present on the three restorative materials ranked in this ascending order: glass-ionomer cement < resin composite < amalgam. Results of DGGE analysis suggested that glass-ionomer cement was the best material of choice in terms of suppressing bacterial phylotypes in biofilms.

语种: 英语
WOS记录号: WOS:000340230900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55854
Appears in Collections:北京大学口腔医学院_口腔生物研究室_期刊论文

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作者单位: 1.Univ Hong Kong, Fac Dent, Sai Ying Pun, Hong Kong, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Oral Biol, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Natl Engn Lab Digital & Mat Technol Stomatol, Dent Mat Lab, Beijing 100081, Peoples R China
4.Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA

Recommended Citation:
Wang, Shuai,Guo, Lihong,Seneviratne, Chaminda Jayampath,et al. Biofilm formation of salivary microbiota on dental restorative materials analyzed by denaturing gradient gel electrophoresis and sequencing[J]. DENTAL MATERIALS JOURNAL,2014,33(3):325-331.
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