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Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease
Qin, YaZhen; Zhu, HongHu; Jiang, Bin; Li, JinLan; Lu, XiJing; Li, LingDi; Ruan, GuoRui; Liu, YanRong; Chen, ShanShan; Huang, XiaoJun
关键词Wt1 Prame Acute Myeloid Leukemia Minimal Residual Disease Real-time Quantitative Pcr
刊名LEUKEMIA RESEARCH
2009-03-01
DOI10.1016/j.leukres.2008.08.026
33期:3页:384-390
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Hematology
研究领域[WOS]Oncology ; Hematology
关键词[WOS]POLYMERASE-CHAIN-REACTION ; TUMOR GENE WT1 ; TIME QUANTITATIVE PCR ; WILMS-TUMOR ; MESSENGER-RNA ; PROGNOSTIC-SIGNIFICANCE ; TRANSCRIPT LEVELS ; CANCER PROGRAM ; DIAGNOSIS ; ANTIGEN
英文摘要

Both WT1 and PRAME are highly expressed in acute myeloid leukemia (AML) patients. To assess the efficacy of their simultaneous detection for the purpose of monitoring minimal residual disease (MRD), we used real-time quantitative RT-PCR to quantify both WT1 and PRAME transcript levels in the bone marrow of 204 newly diagnosed AML patients, and 21 patients were serially monitored for a median of I I months. The 22 normal bone marrow samples which served as controls collectively expressed low levels of WT1 and PRAME. An increase of >1-log over the upper limit of normal bone marrow was defined as positive. The positive rates of WT1 and PRAME for all patients were 79.2% and 55.4%, respectively. For the 108 patients lacking a specific fusion gene, the positive rate of WT1 was significantly higher than that of PRAME (76.9% vs. 35.2%, P<0.00 1). PRAME was positive in 9/25 WT1 (-) patients and the log increase of PRAME was higher than that of WT1 in 12/83 WT1 (+) patients. Dynamic patterns of WT1 and PRAME during follow-up showed that a consistent elevation or a rise over time to exceed the normal range predicted clinical relapse. The exception was that one patient′s WT1 significantly decreased at relapse compared to diagnosis. Therefore, a simultaneous detection of WT1 and PRAME might not only provide AML patients with either a positive or a more sensitive molecular marker for MRD monitoring. Moreover, it might also avoid false negativity in the case of expression alteration. (c) 2008 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000263636700007
项目编号2006AA02A405
资助机构People&prime ; s Hospital of Peking University of China ; National High Technology Research and Development Program of China (863Program)
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55857
专题北京大学第二临床医学院_血液科
北京大学药学院_药事管理与临床药学系
北京大学临床肿瘤学院_移植与免疫治疗病区
作者单位Peking Univ, Peoples Hosp, Inst Hematol, Cellular & Mol Biol Lab, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Qin, YaZhen,Zhu, HongHu,Jiang, Bin,et al. Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease[J]. LEUKEMIA RESEARCH,2009,33(3):384-390.
APA Qin, YaZhen.,Zhu, HongHu.,Jiang, Bin.,Li, JinLan.,Lu, XiJing.,...&Huang, XiaoJun.(2009).Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease.LEUKEMIA RESEARCH,33(3),384-390.
MLA Qin, YaZhen,et al."Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease".LEUKEMIA RESEARCH 33.3(2009):384-390.
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