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学科主题: 临床医学
题名:
Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease
作者: Qin, YaZhen; Zhu, HongHu; Jiang, Bin; Li, JinLan; Lu, XiJing; Li, LingDi; Ruan, GuoRui; Liu, YanRong; Chen, ShanShan; Huang, XiaoJun
关键词: WT1 ; PRAME ; Acute myeloid leukemia ; Minimal residual disease ; Real-time quantitative PCR
刊名: LEUKEMIA RESEARCH
发表日期: 2009-03-01
DOI: 10.1016/j.leukres.2008.08.026
卷: 33, 期:3, 页:384-390
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Hematology
研究领域[WOS]: Oncology ; Hematology
关键词[WOS]: POLYMERASE-CHAIN-REACTION ; TUMOR GENE WT1 ; TIME QUANTITATIVE PCR ; WILMS-TUMOR ; MESSENGER-RNA ; PROGNOSTIC-SIGNIFICANCE ; TRANSCRIPT LEVELS ; CANCER PROGRAM ; DIAGNOSIS ; ANTIGEN
英文摘要:

Both WT1 and PRAME are highly expressed in acute myeloid leukemia (AML) patients. To assess the efficacy of their simultaneous detection for the purpose of monitoring minimal residual disease (MRD), we used real-time quantitative RT-PCR to quantify both WT1 and PRAME transcript levels in the bone marrow of 204 newly diagnosed AML patients, and 21 patients were serially monitored for a median of I I months. The 22 normal bone marrow samples which served as controls collectively expressed low levels of WT1 and PRAME. An increase of >1-log over the upper limit of normal bone marrow was defined as positive. The positive rates of WT1 and PRAME for all patients were 79.2% and 55.4%, respectively. For the 108 patients lacking a specific fusion gene, the positive rate of WT1 was significantly higher than that of PRAME (76.9% vs. 35.2%, P<0.00 1). PRAME was positive in 9/25 WT1 (-) patients and the log increase of PRAME was higher than that of WT1 in 12/83 WT1 (+) patients. Dynamic patterns of WT1 and PRAME during follow-up showed that a consistent elevation or a rise over time to exceed the normal range predicted clinical relapse. The exception was that one patient′s WT1 significantly decreased at relapse compared to diagnosis. Therefore, a simultaneous detection of WT1 and PRAME might not only provide AML patients with either a positive or a more sensitive molecular marker for MRD monitoring. Moreover, it might also avoid false negativity in the case of expression alteration. (c) 2008 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2006AA02A405
项目资助者: People&prime ; s Hospital of Peking University of China ; National High Technology Research and Development Program of China (863Program)
WOS记录号: WOS:000263636700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55857
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Inst Hematol, Cellular & Mol Biol Lab, Beijing 100044, Peoples R China

Recommended Citation:
Qin, YaZhen,Zhu, HongHu,Jiang, Bin,et al. Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease[J]. LEUKEMIA RESEARCH,2009,33(3):384-390.
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