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IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
Blockade of transforming growth factor-beta signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle
作者: Zhang, Qiang; Yang, Ximing J.; Kundu, Shilajit D.; Pins, Michael; Javonovic, Borko; Meyer, Robert; Kim, Seong-Jin; Greenberg, Norman M.; Kuzel, Timothy; Meagher, Richard; Guo, Yinglu; Lee, Chung
刊名: MOLECULAR CANCER THERAPEUTICS
发表日期: 2006-07-01
DOI: 10.1158/1535-7163.MCT-06-0109
卷: 5, 期:7, 页:1733-1743
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: PROSTATE-CANCER CELLS ; GENE-THERAPY ; INFLAMMATORY DISEASE ; ADOPTIVE TRANSFER ; INTERFERON-BETA ; BCL-2 FAMILY ; MICE CAUSES ; SUPPRESSION ; METASTASIS ; ANGIOGENESIS
英文摘要:

Transforming growth factor-beta (TGF-beta) is a potent immunosuppressant. Overproduction of TGF-beta by tumor cells leads to evasion of host immune surveillance and tumor progression. Results of our early studies showed that adoptive transfer of tumor-reactive, TGF-beta-insensitive CD8(+) T cells into immunocompetent mice was able to eradicate lung metastasis of mouse prostate cancer. The present study was conducted with three objectives. (a) We tested if this technology could be applied to the treatment of solid xenograft tumors in allogeneic immunodeficient hosts. (b) We determined relevant variables in the tumor microenvironment with the treatment. (c) We tested if immune cells other than CD8(+) T cells were required for the antitumor effect. Mouse prostate cancer cells, TRAMP-C2 of the C57BL/6 strain, grown in immunodeficient allogeneic hosts of BALB/c strain, were used as a xenograft model. Tumor-reactive CD8+ T cells from C57BL/6 mice were isolated, expanded ex vivo, and rendered insensitive to TGF-beta by introducing a dominant-negative TGF-beta type 11 receptor vector. Seven days following s.c. injection of TRAMP-C2 cells (5 x 10(5)) into the flank of male BALB/c-Rag1(-/-) mice, tumor-reactive, TGF-beta-insensitive CD8(+) T cells (1.5 x 10(7)) were transferred with and without the cotransfer of an equal number of CD8-depleted splenocytes from C57BL/6 donors. Naive CD8(+) T cells or green fluorescent protein-empty vectortransfected tumor-reactive CD8(+) T cells were transferred as controls. Forty days following the transfer, the average tumor weight in animals that received cotransfer of tumor-reactive, TGF-beta-insensitive CD8(+) T cells and CDEI-depleted splenocytes was at least 50% less than that in animals of all other groups (P < 0.05). Tumors in animals of the former group showed a massive infiltration of CD8+ T cells. This was associated with secretion of relevant cytokines, decreased tumor proliferation, reduced angiogenesis, and increased tumor apoptosis. Based on these results, we postulated a concept of antitumor immune response cycle in tumor immunology.

语种: 英语
WOS记录号: WOS:000239682900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55858
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA
2.Peking Univ, Inst Urol, Hosp 1, Beijing 100871, Peoples R China
3.Northwestern Univ, Feinberg Sch Med, Dept Urol, Chicago, IL 60611 USA
4.Northwestern Univ, Feinberg Sch Med, Dept Pathol, Chicago, IL 60611 USA
5.Northwestern Univ, Feinberg Sch Med, Dept Prevent Med, Chicago, IL 60611 USA
6.Northwestern Univ, Feinberg Sch Med, Dept Med, Chicago, IL 60611 USA
7.Northwestern Univ, Feinberg Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA
8.Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA
9.NCI, Lab Cell Regulat & Carcinogenesis, Bethesda, MD 20892 USA

Recommended Citation:
Zhang, Qiang,Yang, Ximing J.,Kundu, Shilajit D.,et al. Blockade of transforming growth factor-beta signaling in tumor-reactive CD8(+) T cells activates the antitumor immune response cycle[J]. MOLECULAR CANCER THERAPEUTICS,2006,5(7):1733-1743.
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