北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 期刊论文
学科主题: 临床医学
题名:
Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells
作者: Li, Qi1; Wang, Jianliu1; Liu, Haifeng2; Xie, Bing1; Wei, Lihui1
关键词: Adipose-derived mesenchymal stem cells ; Pelvic floor reconstruction ; Seed cells ; Silk fibroin scaffold ; Tissue engineering ; Rat
刊名: CELL AND TISSUE RESEARCH
发表日期: 2013-11-01
DOI: 10.1007/s00441-013-1719-2
卷: 354, 期:2, 页:471-480
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: ORGAN PROLAPSE ; IN-VIVO ; URINARY-INCONTINENCE ; STROMAL CELLS ; BONE-MARROW ; BIOMATERIALS ; TENDON ; FIBROBLASTS ; SUPPORT ; SURGERY
英文摘要:

A tissue-engineered mesh fabricated with adipose-derived mesenchymal stem cells (AD-MSCs) cultured on a silk fibroin scaffold is evaluated for use in female pelvic reconstruction. Thirty-five female Sprague Dawley rats were divided into four groups. Group A (n = 10) were implanted with polypropylene meshes, Group B (n = 10) with silk fibroin scaffolds and Group C (n = 10) with tissue-engineered meshes. Group D (n = 5) acted as the tissue control. The tissue-engineered mesh was produced as follows. AD-MSCs were obtained from adipose tissue of rats designated to Group C. The cells were seeded onto a silk fibroin scaffold, cultured and then observed by scanning electron microscopy (SEM). Histological studies of these meshes were performed at 4 and 12 weeks after implantation and mechanical testing was carried out on all groups before implantation and at 12 weeks after implantation. AD-MSCs displayed fibroblast-like shapes and were able to differentiate into adipocytes or fibroblasts. SEM observation showed that AD-MSCs proliferated and secreted a matrix onto the silk fibroin scaffolds. After implantation of the scaffolds into rats, histological analysis revealed better organized newly formed tissue in Group C than in controls. Group C also had a similar failure force (2.67 +/- 0.15 vs 2.33 +/- 0.38 N) and a higher Young′s modulus (2.99 +/- 0.19 vs 1.68 +/- 0.20 MPa) than a normal vaginal wall, indicating the potential of this tissue-engineered approach. AD-MSCs were validated as seed cells for tissue engineering. The silk fibroin scaffold thus shows promise for application with AD-MSCs in the fabrication of tissue-engineered mesh with good biocompatibility and appropriate mechanical properties for pelvic floor reconstruction.

语种: 英语
所属项目编号: 7122194 ; 51917715021801203
项目资助者: Beijing Natural Science Foundation ; Peking University ("985 Project")
WOS记录号: WOS:000326116000012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55872
Appears in Collections:北京大学第二临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Beihang Univ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China

Recommended Citation:
Li, Qi,Wang, Jianliu,Liu, Haifeng,et al. Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells[J]. CELL AND TISSUE RESEARCH,2013,354(2):471-480.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li, Qi]'s Articles
[Wang, Jianliu]'s Articles
[Liu, Haifeng]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li, Qi]‘s Articles
[Wang, Jianliu]‘s Articles
[Liu, Haifeng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2018  北京大学医学部 - Feedback
Powered by CSpace