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学科主题临床医学
Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells
Li, Qi1; Wang, Jianliu1; Liu, Haifeng2; Xie, Bing1; Wei, Lihui1
关键词Adipose-derived Mesenchymal Stem Cells Pelvic Floor Reconstruction Seed Cells Silk Fibroin Scaffold Tissue Engineering Rat
刊名CELL AND TISSUE RESEARCH
2013-11-01
DOI10.1007/s00441-013-1719-2
354期:2页:471-480
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]ORGAN PROLAPSE ; IN-VIVO ; URINARY-INCONTINENCE ; STROMAL CELLS ; BONE-MARROW ; BIOMATERIALS ; TENDON ; FIBROBLASTS ; SUPPORT ; SURGERY
英文摘要

A tissue-engineered mesh fabricated with adipose-derived mesenchymal stem cells (AD-MSCs) cultured on a silk fibroin scaffold is evaluated for use in female pelvic reconstruction. Thirty-five female Sprague Dawley rats were divided into four groups. Group A (n = 10) were implanted with polypropylene meshes, Group B (n = 10) with silk fibroin scaffolds and Group C (n = 10) with tissue-engineered meshes. Group D (n = 5) acted as the tissue control. The tissue-engineered mesh was produced as follows. AD-MSCs were obtained from adipose tissue of rats designated to Group C. The cells were seeded onto a silk fibroin scaffold, cultured and then observed by scanning electron microscopy (SEM). Histological studies of these meshes were performed at 4 and 12 weeks after implantation and mechanical testing was carried out on all groups before implantation and at 12 weeks after implantation. AD-MSCs displayed fibroblast-like shapes and were able to differentiate into adipocytes or fibroblasts. SEM observation showed that AD-MSCs proliferated and secreted a matrix onto the silk fibroin scaffolds. After implantation of the scaffolds into rats, histological analysis revealed better organized newly formed tissue in Group C than in controls. Group C also had a similar failure force (2.67 +/- 0.15 vs 2.33 +/- 0.38 N) and a higher Young′s modulus (2.99 +/- 0.19 vs 1.68 +/- 0.20 MPa) than a normal vaginal wall, indicating the potential of this tissue-engineered approach. AD-MSCs were validated as seed cells for tissue engineering. The silk fibroin scaffold thus shows promise for application with AD-MSCs in the fabrication of tissue-engineered mesh with good biocompatibility and appropriate mechanical properties for pelvic floor reconstruction.

语种英语
WOS记录号WOS:000326116000012
项目编号7122194 ; 51917715021801203
资助机构Beijing Natural Science Foundation ; Peking University ("985 Project")
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55872
专题北京大学第二临床医学院
北京大学第二临床医学院_心血管内科
北京大学第二临床医学院_妇科
作者单位1.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
2.Beihang Univ, Sch Biol Sci & Med Engn, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Qi,Wang, Jianliu,Liu, Haifeng,et al. Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells[J]. CELL AND TISSUE RESEARCH,2013,354(2):471-480.
APA Li, Qi,Wang, Jianliu,Liu, Haifeng,Xie, Bing,&Wei, Lihui.(2013).Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells.CELL AND TISSUE RESEARCH,354(2),471-480.
MLA Li, Qi,et al."Tissue-engineered mesh for pelvic floor reconstruction fabricated from silk fibroin scaffold with adipose-derived mesenchymal stem cells".CELL AND TISSUE RESEARCH 354.2(2013):471-480.
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