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学科主题: 临床医学
题名:
The Alternative Pathway of Complement Activation May Be Involved in the Renal Damage of Human Anti-Glomerular Basement Membrane Disease
作者: Ma, Rui1,2; Cui, Zhao1; Hu, Shui-Yi1; Jia, Xiao-Yu1; Yang, Rui1; Zheng, Xin1; Ao, Jie1; Liu, Gang1; Liao, Yun-Hua2; Zhao, Ming-Hui1,3
刊名: PLOS ONE
发表日期: 2014-03-21
DOI: 10.1371/journal.pone.0091250
卷: 9, 期:3
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PROPERDIN BINDS ; MICE ; GLOMERULONEPHRITIS ; NEPHRITIS ; C3 ; CELLS ; DEFICIENCY ; ARTHRITIS ; ANTIBODY ; DEPOSIT
英文摘要:

Linear deposition of IgG and complement 3 (C3) along glomerular basement membrane (GBM) is generally revealed in the kidneys of human anti-GBM disease. Our recent studies demonstrated the pathogenic role of complement activation in renal damage of this disease. However, the pathways of complement activation were still paradoxical. In this study, renal biopsy tissues from 10 patients with anti-GBM disease were used to investigate the pathways of complement activation by detecting the deposition of various complement components, including C1q, factor B, factor P (properdin), mannose-binding lectin (MBL), C3d, C4d and C5b-9, using immunohistochemistry and immunofluorescence. We found that C1q, factor B, properdin, C3d, C4d and C5b-9 were detected in all the glomeruli of our patients, along GBM with a linear and/or granular staining pattern. Furthermore, C1q, factor B and properdin co-localized well with C5b-9. The properdin also co-localized well with C3d. However, the deposition of MBL was diffusive in mesangium, GBM, Bowman′s capsule and within crescents and was not co-localized with C5b-9 but partially co-localized with C4d. The intensity of factor B deposition (3.3 vs. 1.2, P<0.001) and C5b-9 deposition (3.2 vs. 1.6, P<0.001) was significantly stronger in the glomeruli with crescent formation, compared with the glomeruli without crescents. The complement system is overall activated via both the alternative pathway and classical pathway in the kidneys of human anti-GBM disease. The alternative pathway might play an important role in complement activation induced renal damage.

语种: 英语
所属项目编号: 2012CB517702 ; 81321064 ; 81370801
项目资助者: Chinese 973 project ; Natural Science Fund of China ; National Natural Science Fund of China
WOS记录号: WOS:000333355300029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55879
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Inst Nephrol,Key Lab Renal Dis, Dept Med,Renal Div,Minist Educ China,Key Lab Chro, Beijing 100871, Peoples R China
2.Guangxi Med Univ, Affiliated Hosp 1, Dept Med, Div Renal, Nanning, Guangxi Zhuang, Peoples R China
3.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China

Recommended Citation:
Ma, Rui,Cui, Zhao,Hu, Shui-Yi,et al. The Alternative Pathway of Complement Activation May Be Involved in the Renal Damage of Human Anti-Glomerular Basement Membrane Disease[J]. PLOS ONE,2014,9(3).
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