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学科主题: 临床医学
题名:
Differentially expressed proteins of MCF-7 human breast cancer cells affected by Zilongjin, a complementary Chinese herbal medicine
作者: Tian, Zhi-Hua1; Li, Zheng-Fu1; Zhou, Shao-Bo2; Liang, Yun-Yan1; He, Da-Cheng3,4; Wang, Dai-Shu1
关键词: Differentially expressed proteins ; MCF-7 cells ; Zilongjin
刊名: PROTEOMICS CLINICAL APPLICATIONS
发表日期: 2010-05-01
DOI: 10.1002/prca.200900092
卷: 4, 期:5, 页:550-559
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BINDING PROTEIN ; IN-VITRO ; GENE ; OVEREXPRESSION ; TRANSFORMATION ; TRANSITION ; CARCINOMA ; FAMILY ; D53 ; RAN
英文摘要:

Purpose: Zilongjin, a complementary Chinese herbal medicine, has been used to alleviate the adverse effects of chemotherapeutic drugs in cancer therapy. However, the mechanisms of anti-cancer activity of Zilongjin are still largely unkonwn.

Experimental design: First, the proteomic approach of combined 2-DE and ESI-MS/MS was used to investigate the effect of Zilongjin on the protein expression in MCF-7 cells. Then, the differential expression of some proteins was confirmed by Western blot, cytoimmunofluoresecnce, and quantitative real-time RT-PCR analysis.

Results: The identified proteins with differential expression, involved in such events as protein translation, cellular signal transduction, cytoskeleton formation and transportation, include seven downregulating proteins, such as Eukaryotic translation initiation factor 3 subunit I, Eukaryotic translation initiation factor 1A Y-chromosomal, Ran-specific GTPase-activating protein, Ubiquitin-conjugating enzyme E2 N, Tropomodulin-3, Macrophage-capping protein, and Tumor protein D52, as well as two upregulating proteins, HSP beta-1 and keratin18. Moreover, the differential expression of three proteins was confirmed.

Conclusions and clinical relevance: (i) These results provide a new insight into the molecular mechanisms of Zilongjin on therapy for breast cancer. (ii) The application of the proteomic approaches will result in the more extended appreciation of Chinese medicine than those known at present.

语种: 英语
所属项目编号: 39480026 ; 200603910100
项目资助者: National Sciences Foundation, P. R. China ; special funds for the major state basic research of China
WOS记录号: WOS:000278432700008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55883
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Key Lab Carcinogenesis & Translat Res, Minist Educ,Beijing Canc Hosp & Inst,Cent Lab, Beijing 100142, Peoples R China
2.Univ Bedfordshire, LIRANS Inst Res Appl Nat Sci, Luton, Beds, England
3.Beijing Normal Univ, Minist Educ, Inst Cell Biol, Beijing 100875, Peoples R China
4.Beijing Normal Univ, Minist Educ, Key Lab Cell Proliferat & Regulat Biol, Beijing 100875, Peoples R China

Recommended Citation:
Tian, Zhi-Hua,Li, Zheng-Fu,Zhou, Shao-Bo,et al. Differentially expressed proteins of MCF-7 human breast cancer cells affected by Zilongjin, a complementary Chinese herbal medicine[J]. PROTEOMICS CLINICAL APPLICATIONS,2010,4(5):550-559.
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