IR@PKUHSC  > 北京大学深圳医院
Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer
Li, Yingrui1; Xu, Xun1; Song, Luting1,2,3,4; Hou, Yong1,5,6; Li, Zesong7,8,9; Tsang, Shirley10; Li, Fuqiang1; Im, Kate McGee11; Wu, Kui1; Wu, Hanjie1,12; Ye, Xiaofei1; Li, Guibo1; Wang, Linlin1; Zhang, Bo1; Liang, Jie1; Xie, Wei1,5,6; Wu, Renhua1; Jiang, Hui1; Liu, Xiao1; Yu, Chang1; Zheng, Hancheng1; Jian, Min1; Nie, Liping13; Wan, Lei14; Shi, Min13; Sun, Xiaojuan7,8,9; Tang, Aifa7,8,9; Guo, Guangwu1; Gui, Yaoting13; Cai, Zhiming8,9,13; Li, Jingxiang1; Wang, Wen2; Lu, Zuhong5,6; Zhang, Xiuqing1; Bolund, Lars1,15; Kristiansen, Karsten1,16; Wang, Jian1; Yang, Huanming1; Dean, Michael11; Wang, Jun1,16,17
关键词Single-cell Exome Sequencing Bladder Cancer Tumor Evolution Population Genetics
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics

Background: Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level.

Results: We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively.

Conclusions: This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level.

项目编号2011CB809202 ; 2011CB809203 ; 2009AA022707 ; ZYC201005250020A ; CX B200903 110066A ; CXB201108250096A
资助机构National Basic Research Program of China (973 program) ; Chinese 863 program ; Shenzhen Municipal Government of China ; Key Laboratory Project - Shenzhen City ; Shenzhen Key Laboratory of Gene Bank for National Life Science ; Innovative Research Team Project of GuangDong ; Guangdong Enterprise Key Laboratory of Human Disease Genomics ; Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research ; Danish Natural Science Research Council ; Shenzhen Municipal Government ; Local Government of Yantian District of Shenzhen
被引频次:44[WOS]   [WOS记录]     [WOS相关记录]
作者单位1.Univ Copenhagen, Dept Biol, DK-2200 Copenhagen, Denmark
2.BGI Shenzhen, Beishan Ind Zone, Shenzhen 518083, Peoples R China
3.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, CAS Max Planck Jr Res Grp, Kunming 650223, Yunnan, Peoples R China
4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
5.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
6.Southeast Univ, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China
7.BioMatrix LLC, Rockville, MD 20850 USA
8.Longgang Cent Hosp, Dept Urol, Shenzhen 518116, Peoples R China
9.Univ Aarhus, Inst Human Genet, DK-8100 Aarhus, Denmark
10.Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
11.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen Key Lab Genitourinary Tumor, Shenzhen 518035, Peoples R China
12.Shenzhen Second Peoples Hosp, Dept Urol, Shenzhen 518035, Peoples R China
13.Shenzhen Univ, Inst Urogenital Dis, Shenzhen 518060, Peoples R China
14.NCI, Canc & Inflammat Program, Frederick, MD 21702 USA
15.S China Univ Technol, Guangzhou Higher Educ Mega Ctr, Sch Biosci & Biotechnol, Guangzhou 510006, Guangdong, Peoples R China
16.Peking Univ, Guangdong & Shenzhen Key Lab Male Reprod Med & Ge, Shenzhen PKU HKUST Med Ctr, Inst Urol,Shenzhen Hosp, Shenzhen 518036, Peoples R China
17.Univ Copenhagen, Novo Nordisk Fdn Ctr Basic Metab Res, DK-2200 Copenhagen, Denmark
GB/T 7714
Li, Yingrui,Xu, Xun,Song, Luting,et al. Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer[J]. GIGASCIENCE,2012,1.
APA Li, Yingrui.,Xu, Xun.,Song, Luting.,Hou, Yong.,Li, Zesong.,...&Wang, Jun.(2012).Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer.GIGASCIENCE,1.
MLA Li, Yingrui,et al."Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer".GIGASCIENCE 1(2012).
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