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Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure
Zhang, Fang-Ting; Wan, Hui-Juan; Li, Ming-Hua; Ye, Jing; Yin, Mei-Jun; Huang, Chun-Qiao; Yu, Jie
关键词Microencapsulation Hepatic-like Cells Umbilical Cord Blood Cells Cd34 Antigen Alginate Acute Hepatic Failure
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2011-02-21
DOI10.3748/wjg.v17.i7.938
17期:7页:938-945
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]BONE-MARROW ; STEM-CELLS ; HEPATOCYTES ; MICE ; ISLETS ; LIVER ; RAT
英文摘要

AIM: To investigate intraperitoneal transplantation of microencapsulated hepatic-like cells from human umbilical cord blood for treatment of hepatic failure in rats.

METHODS: CD34(+) cells in umbilical cord blood cells were isolated by magnetic cell sorting. In the in vitro experiment, sorted CD34(+) cells were amplified and induced into hepatic-like cells by culturing with a combination of fibroblast growth factor 4 and hepatocyte growth factor. Cultures without growth factor addition served as controls. mRNA and protein levels for hepatic-like cells were analyzed by reverse transcriptionpolymerase chain reaction, immunohistochemistry and immunofluorescence. In the in vivo experiment, the hepatic-like cells were encapsulated and transplanted into the abdominal cavity of acute hepatic failure (AHF) rats at 48 h after D-galactosamine induction of acute hepatic failure. Transplantation with PBS and unencapsulated hepatic-like cells served as controls. The mortality rate, hepatic pathological changes and serum biochemical indexes were determined. The morphology and structure of microcapsules in the greater omentum were observed.

RESULTS: Human albumin, alpha-fetoprotein and GATA-4 mRNA and albumin protein positive cells were found among cultured cells after 16 d. Albumin level in culture medium was significantly increased after culturing with growth factors in comparison with culturing without growth factor addition (P < 0.01). Compared with the unencapsulated group, the mortality rate of the encapsulated hepatic-like cell-transplanted group was significantly lower (P < 0.05). Serum biochemical parameters, alanine aminotransferase, aspartate aminotransferase and total bilirubin in the encapsulated group were significantly improvement compared with the PBS control group (P < 0.01). Pathological staining further supported these findings. At 1-2 wk post-transplantation, free microcapsules with a round clear structure and a smooth surface were observed in peritoneal lavage fluid, surviving cells inside microcapsules were found by trypan blue staining, but some fibrous tissue around microcapsules was also detected in the greater omentum of encapsulated group by hematoxylin and eosin staining.

CONCLUSION: Transplantation of microencapsulated hepatic-like cells derived from umbilical cord blood cells could preliminarily alleviate the symptoms of AHF rats. (C) 2011 Baishideng. All rights reserved.

语种英语
WOS记录号WOS:000288058800014
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55905
专题北京大学深圳医院_中心实验室
北京大学第三临床医学院_胸外科
作者单位Peking Univ, Cent Lab, Shenzhen Hosp, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Fang-Ting,Wan, Hui-Juan,Li, Ming-Hua,et al. Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2011,17(7):938-945.
APA Zhang, Fang-Ting.,Wan, Hui-Juan.,Li, Ming-Hua.,Ye, Jing.,Yin, Mei-Jun.,...&Yu, Jie.(2011).Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure.WORLD JOURNAL OF GASTROENTEROLOGY,17(7),938-945.
MLA Zhang, Fang-Ting,et al."Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure".WORLD JOURNAL OF GASTROENTEROLOGY 17.7(2011):938-945.
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