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学科主题: 临床医学
题名:
Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure
作者: Zhang, Fang-Ting; Wan, Hui-Juan; Li, Ming-Hua; Ye, Jing; Yin, Mei-Jun; Huang, Chun-Qiao; Yu, Jie
关键词: Microencapsulation ; Hepatic-like cells ; Umbilical cord blood cells ; CD34 antigen ; Alginate ; Acute hepatic failure
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2011-02-21
DOI: 10.3748/wjg.v17.i7.938
卷: 17, 期:7, 页:938-945
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: BONE-MARROW ; STEM-CELLS ; HEPATOCYTES ; MICE ; ISLETS ; LIVER ; RAT
英文摘要:

AIM: To investigate intraperitoneal transplantation of microencapsulated hepatic-like cells from human umbilical cord blood for treatment of hepatic failure in rats.

METHODS: CD34(+) cells in umbilical cord blood cells were isolated by magnetic cell sorting. In the in vitro experiment, sorted CD34(+) cells were amplified and induced into hepatic-like cells by culturing with a combination of fibroblast growth factor 4 and hepatocyte growth factor. Cultures without growth factor addition served as controls. mRNA and protein levels for hepatic-like cells were analyzed by reverse transcriptionpolymerase chain reaction, immunohistochemistry and immunofluorescence. In the in vivo experiment, the hepatic-like cells were encapsulated and transplanted into the abdominal cavity of acute hepatic failure (AHF) rats at 48 h after D-galactosamine induction of acute hepatic failure. Transplantation with PBS and unencapsulated hepatic-like cells served as controls. The mortality rate, hepatic pathological changes and serum biochemical indexes were determined. The morphology and structure of microcapsules in the greater omentum were observed.

RESULTS: Human albumin, alpha-fetoprotein and GATA-4 mRNA and albumin protein positive cells were found among cultured cells after 16 d. Albumin level in culture medium was significantly increased after culturing with growth factors in comparison with culturing without growth factor addition (P < 0.01). Compared with the unencapsulated group, the mortality rate of the encapsulated hepatic-like cell-transplanted group was significantly lower (P < 0.05). Serum biochemical parameters, alanine aminotransferase, aspartate aminotransferase and total bilirubin in the encapsulated group were significantly improvement compared with the PBS control group (P < 0.01). Pathological staining further supported these findings. At 1-2 wk post-transplantation, free microcapsules with a round clear structure and a smooth surface were observed in peritoneal lavage fluid, surviving cells inside microcapsules were found by trypan blue staining, but some fibrous tissue around microcapsules was also detected in the greater omentum of encapsulated group by hematoxylin and eosin staining.

CONCLUSION: Transplantation of microencapsulated hepatic-like cells derived from umbilical cord blood cells could preliminarily alleviate the symptoms of AHF rats. (C) 2011 Baishideng. All rights reserved.

语种: 英语
WOS记录号: WOS:000288058800014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55905
Appears in Collections:北京大学深圳医院_中心实验室_期刊论文

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作者单位: Peking Univ, Cent Lab, Shenzhen Hosp, Shenzhen 518036, Peoples R China

Recommended Citation:
Zhang, Fang-Ting,Wan, Hui-Juan,Li, Ming-Hua,et al. Transplantation of microencapsulated umbilical-cord-blood-derived hepatic-like cells for treatment of hepatic failure[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2011,17(7):938-945.
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