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IR@PKUHSC  > 北京大学第一临床医学院  > 呼吸和危重症医学科  > 期刊论文
学科主题: 临床医学
题名:
Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis
作者: Tan, YX; Han, WL; Chen, YY; Ouyang, NT; Tang, Y; Li, F; Ding, PG; Ren, XL; Zeng, GQ; Ding, J; Zhu, T; Ma, DL; Zhong, NS
关键词: chemokine-like factor 1 ; electroporation ; pathology ; airway remodeling ; asthma ; severe acute respiratory syndrome
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2004-08-01
卷: 117, 期:8, 页:1123-1129
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: ACUTE RESPIRATORY SYNDROME ; MEDIATED GENE-TRANSFER ; SKELETAL-MUSCLE ; LUNG PATHOLOGY ; GROWTH-FACTORS ; SYNDROME SARS ; IN-VIVO ; ASTHMA ; ELECTROPORATION ; PROLIFERATION
英文摘要:

Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine. The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines. Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells. We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung. Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung.

Methods CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation. Lung tissues were obtained at the end of week 1 2, 3 and 4 respectively after injection. The pathological changes in the lungs were observed by light microscope.

Results A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice. These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung.

Conclusions The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome. These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electro-transfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals.

语种: 英语
WOS记录号: WOS:000223711500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55908
Appears in Collections:北京大学第一临床医学院_呼吸和危重症医学科_期刊论文

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作者单位: 1.First Affiliated Hosp, Guangzhou Inst Resp Dis, Guangzhou Med Coll, Guangzhou 510120, Peoples R China
2.Peking Univ, Affiliated Hosp 1, Dept Resp Dis, Beijing 100034, Peoples R China
3.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China

Recommended Citation:
Tan, YX,Han, WL,Chen, YY,et al. Chemokine-like factor 1, a novel cytokine, contributes to airway damage, remodeling and pulmonary fibrosis[J]. CHINESE MEDICAL JOURNAL,2004,117(8):1123-1129.
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