IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71
Wu, Xing1,2,3; Mao, Qunying3; Yao, Xin3; Chen, Pan4; Chen, Xiangmei1,2; Shao, Jie3; Gao, Fan3; Yu, Xiang5; Zhu, Fengcai6; Li, Rongcheng7; Li, Wenhui4; Liang, Zhenglun3; Wang, Junzhi3; Lu, Fengmin1,2
刊名PLOS ONE
2013-06-05
DOI10.1371/journal.pone.0064116
8期:6
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]COXSACKIEVIRUS A16 ; INFECTION ; VIRUS ; TAIWAN ; ANTIGENICITY ; STRAINS ; VACCINE ; MICE ; IMMUNIZATION ; VALIDATION
英文摘要

The level of neutralizing antibodies (NtAb) induced by vaccine inoculation is an important endpoint to evaluate the efficacy of EV71 vaccine. In order to evaluate the efficacy of EV71 vaccine, here, we reported the development of a novel pseudovirus system expression firefly luciferase (PVLA) for the quantitative measurement of NtAb. We first evaluated and validated the sensitivity and specificity of the PVLA method. A total of 326 serum samples from an epidemiological survey and 144 serum specimens from 3 clinical trials of EV71 vaccines were used, and the level of each specimen′s neutralizing antibodies (NtAb) was measured in parallel using both the conventional CPE-based and PVLA-based assay. Against the standard neutralization assay based on the inhibition of the cytopathic effect (CPE), the sensitivity and specificity of the PVLA method are 98% and 96%, respectively. Then, we tested the potential interference of NtAb against hepatitis A virus, Polio-I, Polio-II, and Polio-III standard antisera (WHO) and goat anti-G10/CA16 serum, the PVLA based assay showed no cross-reactivity with NtAb against other specific sera. Importantly, unlike CPE based method, no live replication-competent EV71 is used during the measurement. Taken together, PVLA is a rapid and specific assay with higher sensitivity and accuracy. It could serve as a valuable tool in assessing the efficacy of EV71 vaccines in clinical trials and disease surveillance in epidemiology studies.

语种英语
WOS记录号WOS:000320579400008
项目编号2008BAI69B01 ; 2009ZX10004-804
资助机构National Science Project ; National 11th Five Major Special Projects Funding Program
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55913
专题北京大学基础医学院_病原生物学系
作者单位1.Hualan Biol Engn Inc, Xinxiang, Henan, Peoples R China
2.Natl Inst Biol Sci, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Beijing 100871, Peoples R China
5.Minist Hlth Res Qual & Standardizat Biotech Prod, Natl Inst Food & Drug Control, Dept Hepatitis Vaccine, Key Lab, Beijing, Peoples R China
6.Jiangsu Prov Ctr Dis Prevent & Control, Nanjing, Jiangsu, Peoples R China
7.Guangxi Zhuang Automomous Reg, Ctr Dis Control & Prevent, Nanning, Peoples R China
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GB/T 7714
Wu, Xing,Mao, Qunying,Yao, Xin,et al. Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71[J]. PLOS ONE,2013,8(6).
APA Wu, Xing.,Mao, Qunying.,Yao, Xin.,Chen, Pan.,Chen, Xiangmei.,...&Lu, Fengmin.(2013).Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71.PLOS ONE,8(6).
MLA Wu, Xing,et al."Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71".PLOS ONE 8.6(2013).
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