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学科主题: 基础医学
题名:
Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71
作者: Wu, Xing1,2,3; Mao, Qunying3; Yao, Xin3; Chen, Pan4; Chen, Xiangmei1,2; Shao, Jie3; Gao, Fan3; Yu, Xiang5; Zhu, Fengcai6; Li, Rongcheng7; Li, Wenhui4; Liang, Zhenglun3; Wang, Junzhi3; Lu, Fengmin1,2
刊名: PLOS ONE
发表日期: 2013-06-05
DOI: 10.1371/journal.pone.0064116
卷: 8, 期:6
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: COXSACKIEVIRUS A16 ; INFECTION ; VIRUS ; TAIWAN ; ANTIGENICITY ; STRAINS ; VACCINE ; MICE ; IMMUNIZATION ; VALIDATION
英文摘要:

The level of neutralizing antibodies (NtAb) induced by vaccine inoculation is an important endpoint to evaluate the efficacy of EV71 vaccine. In order to evaluate the efficacy of EV71 vaccine, here, we reported the development of a novel pseudovirus system expression firefly luciferase (PVLA) for the quantitative measurement of NtAb. We first evaluated and validated the sensitivity and specificity of the PVLA method. A total of 326 serum samples from an epidemiological survey and 144 serum specimens from 3 clinical trials of EV71 vaccines were used, and the level of each specimen′s neutralizing antibodies (NtAb) was measured in parallel using both the conventional CPE-based and PVLA-based assay. Against the standard neutralization assay based on the inhibition of the cytopathic effect (CPE), the sensitivity and specificity of the PVLA method are 98% and 96%, respectively. Then, we tested the potential interference of NtAb against hepatitis A virus, Polio-I, Polio-II, and Polio-III standard antisera (WHO) and goat anti-G10/CA16 serum, the PVLA based assay showed no cross-reactivity with NtAb against other specific sera. Importantly, unlike CPE based method, no live replication-competent EV71 is used during the measurement. Taken together, PVLA is a rapid and specific assay with higher sensitivity and accuracy. It could serve as a valuable tool in assessing the efficacy of EV71 vaccines in clinical trials and disease surveillance in epidemiology studies.

语种: 英语
所属项目编号: 2008BAI69B01 ; 2009ZX10004-804
项目资助者: National Science Project ; National 11th Five Major Special Projects Funding Program
WOS记录号: WOS:000320579400008
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55913
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Hualan Biol Engn Inc, Xinxiang, Henan, Peoples R China
2.Natl Inst Biol Sci, Beijing, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Ctr Infect Dis, Beijing 100871, Peoples R China
5.Minist Hlth Res Qual & Standardizat Biotech Prod, Natl Inst Food & Drug Control, Dept Hepatitis Vaccine, Key Lab, Beijing, Peoples R China
6.Jiangsu Prov Ctr Dis Prevent & Control, Nanjing, Jiangsu, Peoples R China
7.Guangxi Zhuang Automomous Reg, Ctr Dis Control & Prevent, Nanning, Peoples R China

Recommended Citation:
Wu, Xing,Mao, Qunying,Yao, Xin,et al. Development and Evaluation of a Pseudovirus-Luciferase Assay for Rapid and Quantitative Detection of Neutralizing Antibodies against Enterovirus 71[J]. PLOS ONE,2013,8(6).
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