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学科主题: 口腔医学
题名:
Synergistic Effects of Nanosecond Pulsed Electric Fields Combined with Low Concentration of Gemcitabine on Human Oral Squamous Cell Carcinoma In Vitro
作者: Wang, Jing1; Guo, Jinsong1,2; Wu, Shan2; Feng, Hongqing2; Sun, Shujun3; Pan, Jie4; Zhang, Jue2,3; Beebe, Stephen J.5
刊名: PLOS ONE
发表日期: 2012-08-23
DOI: 10.1371/journal.pone.0043213
卷: 7, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: ELECTROPORATION THERAPY ; NECK-CANCER ; INDUCE APOPTOSIS ; HEAD ; MECHANISMS ; CALCIUM ; DEATH ; DRUG ; TRANSLOCATION ; INHIBITION
英文摘要:

Treatment of cancer often involves uses of multiple therapeutic strategies with different mechanisms of action. In this study we investigated combinations of nanosecond pulsed electric fields (nsPEF) with low concentrations of gemcitabine on human oral cancer cells. Cells (Cal-27) were treated with pulse parameters (20 pulses, 100 ns in duration, intensities of 10, 30 and 60 kV/cm) and then cultured in medium with 0.01 mu g/ml gemcitabine. Proliferation, apoptosis/necrosis, invasion and morphology of those cells were examined using MTT, flow cytometry, clonogenics, transwell migration and TEM assay. Results show that combination treatments of gemcitabine and nsPEFs exhibited significant synergistic activities versus individual treatments for inhibiting oral cancer cell proliferation and inducing apoptosis and necrosis. However, there was no apparent synergism for cell invasion. By this we demonstrated synergistic inhibition of Cal-27 cells in vitro by nsPEFs and gemcitabine. Synergistic behavior indicates that these two treatments have different sites of action and combination treatment allows reduced doses of gemcitabine and lower nsPEF conditions, which may provide better treatment for patients than either treatment alone while reducing systemic toxicities.

语种: 英语
所属项目编号: GZK-2011-78 ; lzujbky-2012-159
项目资助者: Gansu Chinese Medicine Funds ; Fundamental Research Funds for the Central Universities ; Mr. Fran Reidy, Bioelectrics Inc.
WOS记录号: WOS:000308224700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55915
Appears in Collections:北京大学口腔医学院_综合治疗科_期刊论文

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作者单位: 1.Peking Univ, Coll Engn, Beijing 100871, Peoples R China
2.Lanzhou Univ, Dept Oral Med, Sch Stomatol, Lanzhou, Gansu, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
4.Peking Univ, Sch Stomatol, Dept Gen Dent, Beijing 100871, Peoples R China
5.Old Dominion Univ, Frank Reidy Res Ctr Bioelect, Norfolk, VA USA

Recommended Citation:
Wang, Jing,Guo, Jinsong,Wu, Shan,et al. Synergistic Effects of Nanosecond Pulsed Electric Fields Combined with Low Concentration of Gemcitabine on Human Oral Squamous Cell Carcinoma In Vitro[J]. PLOS ONE,2012,7(8).
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