IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科
学科主题临床医学
Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma
Zhang, Q; Zhang, ZW; Gong, K; Xin, DQ; Na, YQ; Guo, YL
关键词Kidney Neoplasms Carcinoma Suppression Subtractive Hybridization Gene Library
刊名CHINESE SCIENCE BULLETIN
2001-02-01
46期:3页:226-229
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]C-MYC
英文摘要

Using suppression subtractive hybridization, a renal cell carcinoma (RCC) cDNA subtractive library which only contains differently expressed cDNAs between human RCC and normal kidney has been constructed, 200 clones were picked out randomly to perform enzyme digest analysis, a part Of them underwent sequence analysis and Northern blot to:identify RCC specially expressed genes. Results showed that 190 clones contain 50-400 bp inserts respectively, Sequence analysis was performed in 10 clones. All the 10 sequences were unknown before and derived from 6 unique novel genes among which the cDNA insert RCC18 has five copies. Northern blot analysis showed that RCC18 cDNA expressed highly in RCC, but there was no signal detected in normal kidney, and the full length of RCC18 was about 2.5 kb. The constructed cDNA subtractive library of human RCC is a highly efficient one and lays the solid foundation for large-scale screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel specially expressed genes provided an important clue for researching the mechanism of the occurrence and development of RCC.

语种英语
WOS记录号WOS:000167179800015
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55934
专题北京大学第一临床医学院_泌尿外科
作者单位1.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Med Univ, First Affiliated Hosp, Dept Urol, Beijing 100034, Peoples R China
3.Beijing Med Univ, First Affiliated Hosp, Inst Urol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Q,Zhang, ZW,Gong, K,et al. Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma[J]. CHINESE SCIENCE BULLETIN,2001,46(3):226-229.
APA Zhang, Q,Zhang, ZW,Gong, K,Xin, DQ,Na, YQ,&Guo, YL.(2001).Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma.CHINESE SCIENCE BULLETIN,46(3),226-229.
MLA Zhang, Q,et al."Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma".CHINESE SCIENCE BULLETIN 46.3(2001):226-229.
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