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学科主题: 临床医学
题名:
Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma
作者: Zhang, Q; Zhang, ZW; Gong, K; Xin, DQ; Na, YQ; Guo, YL
关键词: kidney neoplasms ; carcinoma ; suppression ; subtractive hybridization ; gene ; library
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2001-02-01
卷: 46, 期:3, 页:226-229
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: C-MYC
英文摘要:

Using suppression subtractive hybridization, a renal cell carcinoma (RCC) cDNA subtractive library which only contains differently expressed cDNAs between human RCC and normal kidney has been constructed, 200 clones were picked out randomly to perform enzyme digest analysis, a part Of them underwent sequence analysis and Northern blot to:identify RCC specially expressed genes. Results showed that 190 clones contain 50-400 bp inserts respectively, Sequence analysis was performed in 10 clones. All the 10 sequences were unknown before and derived from 6 unique novel genes among which the cDNA insert RCC18 has five copies. Northern blot analysis showed that RCC18 cDNA expressed highly in RCC, but there was no signal detected in normal kidney, and the full length of RCC18 was about 2.5 kb. The constructed cDNA subtractive library of human RCC is a highly efficient one and lays the solid foundation for large-scale screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel specially expressed genes provided an important clue for researching the mechanism of the occurrence and development of RCC.

语种: 英语
WOS记录号: WOS:000167179800015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55934
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Beijing Med Univ, Dept Physiol, Beijing 100083, Peoples R China
2.Beijing Med Univ, First Affiliated Hosp, Dept Urol, Beijing 100034, Peoples R China
3.Beijing Med Univ, First Affiliated Hosp, Inst Urol, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, Q,Zhang, ZW,Gong, K,et al. Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma[J]. CHINESE SCIENCE BULLETIN,2001,46(3):226-229.
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