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The cell cycle distribution should be given more consideration in cell-based in vitro toxicological studies
Zhao, Peng1,2; Fu, Juanling1; Yao, Biyun1,2; Song, Yanchao1,2; Yuan, Lan3; Jia, Yongrui3; Ma, Shiliang3; Chen, Wen4; Zhou, Zongcan1
关键词Cell Cycle Distribution Confounding Cell-based In Vitro Assay Diethyl Sulfate Hbe Cells
刊名ARCHIVES OF TOXICOLOGY
2014-02-01
DOI10.1007/s00204-013-1103-8
88期:2页:337-343
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
资助者National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Fundamental Research Funds for the Central Universities
研究领域[WOS]Toxicology
关键词[WOS]EXPRESSION PATTERNS ; PROTEOMIC ANALYSIS ; MECHANISMS ; ARREST ; G0/G1
英文摘要

In this study, to discuss the importance of the cell cycle distribution in cell-based in vitro toxicity mechanism studies, diethyl sulfate (DES) was selected as a model chemical that induced the alteration of the cell cycle distribution in human bronchial epithelial cell line 16HBE 14o- (HBE) cells. Cells were treated with various concentrations of DES, cell proliferation and apoptosis were then determined. The results showed that DES concentration-dependently inhibited HBE cells proliferation and induced apoptosis. When cells were treated with 2.0 mM of DES for 20 or 28 h, significant S and G2/M phase accumulation was observed. Then, the relative cellular levels of Cdk4, p-Cdk2 (Thr160), Cyclins A and B1 in DES-treated HBE cells at 20 and 28 h were determined by two ways. The differences of the cell cycle distribution between DES and control groups were ignored in one way and eliminated by using flow cytometric cell sorting in the other. The results obtained by the two ways were quite different, which indicated that the cell cycle distribution might result in confounding if it was significantly different between the treated and control groups. Therefore, we propose that the cell cycle distribution should be given more consideration in cell-based in vitro toxicological studies.

语种英语
所属项目编号81001253 ; 7132122 ; BMU20090460
资助者National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Fundamental Research Funds for the Central Universities
WOS记录号WOS:000330959400015
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55948
专题北京大学公共卫生学院_毒理学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Toxicol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Beijing Key Lab Toxicol Res & Risk Assessment Foo, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Med & Hlth Analyt Ctr, Beijing 100191, Peoples R China
4.Sun Yat Sen Univ, Sch Publ Hlth, Fac Prevent Med, Dept Toxicol, Guangzhou 510080, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Peng,Fu, Juanling,Yao, Biyun,et al. The cell cycle distribution should be given more consideration in cell-based in vitro toxicological studies[J]. ARCHIVES OF TOXICOLOGY,2014,88(2):337-343.
APA Zhao, Peng.,Fu, Juanling.,Yao, Biyun.,Song, Yanchao.,Yuan, Lan.,...&Zhou, Zongcan.(2014).The cell cycle distribution should be given more consideration in cell-based in vitro toxicological studies.ARCHIVES OF TOXICOLOGY,88(2),337-343.
MLA Zhao, Peng,et al."The cell cycle distribution should be given more consideration in cell-based in vitro toxicological studies".ARCHIVES OF TOXICOLOGY 88.2(2014):337-343.
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