IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Altered serum N-glycomics in chronic hepatitis B patients
Gui, Hong-lian2; Gao, Chun-fang3; Wang, Hui2; Liu, Xue-en1,4,5; Xie, Qing2; Dewaele, Sylviane1,4; Wang, Ling5; Zhuang, Hui5; Contreras, Roland1,4; Libert, Claude1,4; Chen, Cuiying1,4
关键词cirrhosis glycomics HBV liver fibrosis N-glycan non-invasive
刊名LIVER INTERNATIONAL
2010-02-01
DOI10.1111/j.1478-3231.2009.02170.x
30期:2页:259-267
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]LIVER FIBROSIS ; HEPATOCELLULAR-CARCINOMA ; TRANSIENT ELASTOGRAPHY ; PROTEIN GLYCOMICS ; PREDICTIVE MODEL ; AGALACTOSYL IGG ; CIRRHOSIS ; GLYCOSYLATION ; DIAGNOSIS ; BIOPSY
英文摘要

Background

We previously reported on serum N-glycans as markers for the diagnosis of cirrhosis in patients with chronic hepatitis C infection. Our present study aimed to evaluate the use of serum glycan markers for the diagnosis of liver fibrosis in patients with chronic hepatitis B infection.

Methods

Patients with hepatitis B virus (HBV) infection (n=173) were diagnosed by clinical laboratory analysis and histological examination. Liver fibrosis was staged using Ishak score. N-glycan profiles of serum proteins were determined by DNA sequencer-based carbohydrate analytical profiling.

Results

We found that in HBV patients, like in hepatitis C virus patients, several serum N-glycans were altered during the development of liver fibrosis. We found higher levels of total agalactosylated biantennary glycans in fibrosis patients with HBV infection than in healthy controls. The biantennary (NA2) and the triantennary (NA3) N-glycans decreased significantly (P < 0.001) with increased severity of fibrosis. The diagnostic power of serum glycan marker (GlycoFibroTest) [area under the curve (AUC)=0.735) was similar to that of FibroTest (AUC=0.740) for discriminating between moderate and advanced fibrosis (F3-F6) from non- or mild fibrosis (F0-F2). However, GlycoFibroTest (AUC=0.740) was slightly better than FibroTest (AUC=0.696) for distinguishing fibrotic patients (F1 or more) from non-fibrotic patients (F0).

Conclusions

The assay for serum glycan profiling showed satisfactory reproducibility and is a non-invasive blood test for the diagnosis of liver fibrosis. The changes of N-glycan level in serum can be used to monitor or follow-up the progress of fibrosis using specific N-glycan markers.

语种英语
WOS记录号WOS:000273021300014
项目编号011S605 ; BL/02/C42 ; 30971345 ; 30571774 ; 09XD1405800 ; 2008ZX10002-005 ; 2008ZX10002-007 ; 2008ZX09312-007 ; 064119521
资助机构Flanders-China Bilateral projects ; Natural Science Foundation of China (NSFC) ; Science and Technology Commission of Shanghai ; National Eleven-five project of China ; Science and Technology of Shanghai Municipal Government
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55950
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
作者单位1.VIB & Ghent Univ, Dept Mol Biomed Res, B-9052 Ghent, Belgium
2.Jiao Tong Univ, Dept Infect Dis, Sch Med, Ruijin Hosp, Shanghai 200030, Peoples R China
3.Second Mil Med Univ, Eastern Hepatobiliary Hosp, Dept Lab Med, Shanghai, Peoples R China
4.Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Gui, Hong-lian,Gao, Chun-fang,Wang, Hui,et al. Altered serum N-glycomics in chronic hepatitis B patients[J]. LIVER INTERNATIONAL,2010,30(2):259-267.
APA Gui, Hong-lian.,Gao, Chun-fang.,Wang, Hui.,Liu, Xue-en.,Xie, Qing.,...&Chen, Cuiying.(2010).Altered serum N-glycomics in chronic hepatitis B patients.LIVER INTERNATIONAL,30(2),259-267.
MLA Gui, Hong-lian,et al."Altered serum N-glycomics in chronic hepatitis B patients".LIVER INTERNATIONAL 30.2(2010):259-267.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Altered serum N-glyc(314KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Gui, Hong-lian]的文章
[Gao, Chun-fang]的文章
[Wang, Hui]的文章
百度学术
百度学术中相似的文章
[Gui, Hong-lian]的文章
[Gao, Chun-fang]的文章
[Wang, Hui]的文章
必应学术
必应学术中相似的文章
[Gui, Hong-lian]的文章
[Gao, Chun-fang]的文章
[Wang, Hui]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Altered serum N-glycomics in chronic hepatitis B patients.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。