北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Altered serum N-glycomics in chronic hepatitis B patients
作者: Gui, Hong-lian2; Gao, Chun-fang3; Wang, Hui2; Liu, Xue-en1,4,5; Xie, Qing2; Dewaele, Sylviane1,4; Wang, Ling5; Zhuang, Hui5; Contreras, Roland1,4; Libert, Claude1,4; Chen, Cuiying1,4
关键词: cirrhosis ; glycomics ; HBV ; liver fibrosis ; N-glycan ; non-invasive
刊名: LIVER INTERNATIONAL
发表日期: 2010-02-01
DOI: 10.1111/j.1478-3231.2009.02170.x
卷: 30, 期:2, 页:259-267
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: LIVER FIBROSIS ; HEPATOCELLULAR-CARCINOMA ; TRANSIENT ELASTOGRAPHY ; PROTEIN GLYCOMICS ; PREDICTIVE MODEL ; AGALACTOSYL IGG ; CIRRHOSIS ; GLYCOSYLATION ; DIAGNOSIS ; BIOPSY
英文摘要:

Background

We previously reported on serum N-glycans as markers for the diagnosis of cirrhosis in patients with chronic hepatitis C infection. Our present study aimed to evaluate the use of serum glycan markers for the diagnosis of liver fibrosis in patients with chronic hepatitis B infection.

Methods

Patients with hepatitis B virus (HBV) infection (n=173) were diagnosed by clinical laboratory analysis and histological examination. Liver fibrosis was staged using Ishak score. N-glycan profiles of serum proteins were determined by DNA sequencer-based carbohydrate analytical profiling.

Results

We found that in HBV patients, like in hepatitis C virus patients, several serum N-glycans were altered during the development of liver fibrosis. We found higher levels of total agalactosylated biantennary glycans in fibrosis patients with HBV infection than in healthy controls. The biantennary (NA2) and the triantennary (NA3) N-glycans decreased significantly (P < 0.001) with increased severity of fibrosis. The diagnostic power of serum glycan marker (GlycoFibroTest) [area under the curve (AUC)=0.735) was similar to that of FibroTest (AUC=0.740) for discriminating between moderate and advanced fibrosis (F3-F6) from non- or mild fibrosis (F0-F2). However, GlycoFibroTest (AUC=0.740) was slightly better than FibroTest (AUC=0.696) for distinguishing fibrotic patients (F1 or more) from non-fibrotic patients (F0).

Conclusions

The assay for serum glycan profiling showed satisfactory reproducibility and is a non-invasive blood test for the diagnosis of liver fibrosis. The changes of N-glycan level in serum can be used to monitor or follow-up the progress of fibrosis using specific N-glycan markers.

语种: 英语
所属项目编号: 011S605 ; BL/02/C42 ; 30971345 ; 30571774 ; 09XD1405800 ; 2008ZX10002-005 ; 2008ZX10002-007 ; 2008ZX09312-007 ; 064119521
项目资助者: Flanders-China Bilateral projects ; Natural Science Foundation of China (NSFC) ; Science and Technology Commission of Shanghai ; National Eleven-five project of China ; Science and Technology of Shanghai Municipal Government
WOS记录号: WOS:000273021300014
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55950
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Altered serum N-glycomics in chronic hepatitis B patients.pdf(314KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.VIB & Ghent Univ, Dept Mol Biomed Res, B-9052 Ghent, Belgium
2.Jiao Tong Univ, Dept Infect Dis, Sch Med, Ruijin Hosp, Shanghai 200030, Peoples R China
3.Second Mil Med Univ, Eastern Hepatobiliary Hosp, Dept Lab Med, Shanghai, Peoples R China
4.Univ Ghent, Dept Biomed Mol Biol, B-9000 Ghent, Belgium
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China

Recommended Citation:
Gui, Hong-lian,Gao, Chun-fang,Wang, Hui,et al. Altered serum N-glycomics in chronic hepatitis B patients[J]. LIVER INTERNATIONAL,2010,30(2):259-267.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Gui, Hong-lian]'s Articles
[Gao, Chun-fang]'s Articles
[Wang, Hui]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Gui, Hong-lian]‘s Articles
[Gao, Chun-fang]‘s Articles
[Wang, Hui]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace