IR@PKUHSC  > 北京大学药学院  > 药剂学系
学科主题药学
Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles
Zhao, Xia; Yu, Shi-Bing; Wu, Feng-Lan; Mao, Ze-Bin; Yu, Chang-Long
关键词Chitosan Nanoparticles Non-viral Gene Delivery Chondrocytes
刊名JOURNAL OF CONTROLLED RELEASE
2006-05-15
DOI10.1016/j.jconrel.2006.01.016
112期:2页:223-228
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]MEDIATED GENE-TRANSFER ; IN-VIVO ; ARTICULAR CHONDROCYTES ; DELIVERY-SYSTEM ; OSTEOCHONDRAL INJURIES ; TRANSGENE EXPRESSION ; DNA NANOPARTICLES ; MAMMALIAN-CELLS ; MOLECULAR-MASS ; THERAPY
英文摘要

Chitosan-pEGFP nanoparticles were synthesized through the complex coacervation of the cationic polymer with pEGFP, in order to examine the potential of chitosan as a non-viral gene delivery vector to transfer exogenous gene into primary chondrocytes for the treatment of joint diseases. The nanoparticles were prepared at an NIP ratio of 3.8 and showed a spherical or irregular shape. The mean particle size and zeta potential of the nanoparticles freshly prepared with chitosan of different molecular weight were in the range of 100-300 nm and varied from + 1 to + 23 mV respectively. Both the particle size and the zeta potential altered in DMEM of different pH. The transfection of primary chondrocytes was performed in different conditions by varying pH of transfection medium, molecular weight of chitosan and different plasmid dosage. Analysis of FACS demonstrated that the transfection efficiency could reach a much high level and the percentage of positive cells could exceed 50% in certain condition. These results suggest that chitosan-DNA nanoparticles have favorable characteristics for non-viral gene delivery to primary chondrocytes. and have the potential to deliver therapeutic genes directly into joint. (c) 2006 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000237908100009
引用统计
被引频次:59[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/55962
专题北京大学药学院_药剂学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Xia,Yu, Shi-Bing,Wu, Feng-Lan,et al. Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles[J]. JOURNAL OF CONTROLLED RELEASE,2006,112(2):223-228.
APA Zhao, Xia,Yu, Shi-Bing,Wu, Feng-Lan,Mao, Ze-Bin,&Yu, Chang-Long.(2006).Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles.JOURNAL OF CONTROLLED RELEASE,112(2),223-228.
MLA Zhao, Xia,et al."Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles".JOURNAL OF CONTROLLED RELEASE 112.2(2006):223-228.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Xia]的文章
[Yu, Shi-Bing]的文章
[Wu, Feng-Lan]的文章
百度学术
百度学术中相似的文章
[Zhao, Xia]的文章
[Yu, Shi-Bing]的文章
[Wu, Feng-Lan]的文章
必应学术
必应学术中相似的文章
[Zhao, Xia]的文章
[Yu, Shi-Bing]的文章
[Wu, Feng-Lan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。