北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 药剂学系  > 期刊论文
学科主题: 药学
题名:
Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles
作者: Zhao, Xia; Yu, Shi-Bing; Wu, Feng-Lan; Mao, Ze-Bin; Yu, Chang-Long
关键词: chitosan ; nanoparticles ; non-viral gene delivery ; chondrocytes
刊名: JOURNAL OF CONTROLLED RELEASE
发表日期: 2006-05-15
DOI: 10.1016/j.jconrel.2006.01.016
卷: 112, 期:2, 页:223-228
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: MEDIATED GENE-TRANSFER ; IN-VIVO ; ARTICULAR CHONDROCYTES ; DELIVERY-SYSTEM ; OSTEOCHONDRAL INJURIES ; TRANSGENE EXPRESSION ; DNA NANOPARTICLES ; MAMMALIAN-CELLS ; MOLECULAR-MASS ; THERAPY
英文摘要:

Chitosan-pEGFP nanoparticles were synthesized through the complex coacervation of the cationic polymer with pEGFP, in order to examine the potential of chitosan as a non-viral gene delivery vector to transfer exogenous gene into primary chondrocytes for the treatment of joint diseases. The nanoparticles were prepared at an NIP ratio of 3.8 and showed a spherical or irregular shape. The mean particle size and zeta potential of the nanoparticles freshly prepared with chitosan of different molecular weight were in the range of 100-300 nm and varied from + 1 to + 23 mV respectively. Both the particle size and the zeta potential altered in DMEM of different pH. The transfection of primary chondrocytes was performed in different conditions by varying pH of transfection medium, molecular weight of chitosan and different plasmid dosage. Analysis of FACS demonstrated that the transfection efficiency could reach a much high level and the percentage of positive cells could exceed 50% in certain condition. These results suggest that chitosan-DNA nanoparticles have favorable characteristics for non-viral gene delivery to primary chondrocytes. and have the potential to deliver therapeutic genes directly into joint. (c) 2006 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000237908100009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/55962
Appears in Collections:北京大学药学院_药剂学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100083, Peoples R China
3.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100083, Peoples R China

Recommended Citation:
Zhao, Xia,Yu, Shi-Bing,Wu, Feng-Lan,et al. Transfection of primary chondrocytes using chitosan-pEGFP nanoparticles[J]. JOURNAL OF CONTROLLED RELEASE,2006,112(2):223-228.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Xia]'s Articles
[Yu, Shi-Bing]'s Articles
[Wu, Feng-Lan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Xia]‘s Articles
[Yu, Shi-Bing]‘s Articles
[Wu, Feng-Lan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace